Thank you for contacting us.

Please find the assay protocol for this kit attached. The lot you specified expired in December 2011, and would no longer be covered under our Abpromise guarantee. It is possible that the reagents may still be working, but this would need to be tested. I hope this helps, please let me know if you need any additional information.

1.) The total number of test wells which can be performed with this kit is 100 (some of those test wells will then be occupied for using the positive and negative controls as well as the standards and blanks) 2.) There is enough positive and negative control to run 5 separate experiments (e.g. 5 times 20 wells in total then)

Thank you for your inquiry. This assay kit can be used with mammalian species, so yes it can be used with the indicated samples. I hope this information helps. Please contact us with any other questions.

Voici la reponse du laboratoire: "It depends on the user's purpose. There may be some enzymes in the cytosol that may interfere with the reaction results if the cell extracts were used. That is why we suggest the use of the nuclear extract." J'espere que cette reponse vous aidera.

Thank you for your enquiry. The buffer contains Tris, NaCl, KCl, and MgCl2. I hope this information helps. Please do not hesitate to contact us again if you need anything further.

Here is a protocol for getting a nuclear extract that will work for the HDAC activity assay: https://www.abcam.com/assets/pdf/protocols/Nuclear%20fractionation%20protocol.pdf I do not know what kind of plate reader you have, but if it cannot measure fluorescence, you should get a colorimetric kit for the HDAC assay, ab1432: https://www.abcam.com/HDAC-Activity-Assay-Kit-colorimetric-ab1432.html I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your email. You want to read and record the fluorescence as measured at an excitation (Ex.) of 350-380 nm and emission (Em.) of 440-460 nm. You can simply express the results as Relative fluorescence units/ug protein sample. The originator of this kit has said that "These values seem to be really low for fluorometric assay. Please check the machine setting to make sure it's correct." If you have any additional questions, please let us know.

Thank you for your email. I'm afraid that we are unable to provide discounts. If you have any additional questions, please let us know.

Thank you for your phone call. The originator of these two kits was able to provide me with the following information. Ab1438 (the fluorometric assay) is ~5 times more sensitive than ab1432 (the colorimetric assay). The HDAC activity assay detects all HDACs (including HDAC-3) that can deacetylate histone. A direct comparison with radioactive detection has not been done. Both ab1432 and ab1438 are currently out of stock and would take approximately 1-2 weeks to receive upon ordering. If you have any additional questions, please contact us again.