Anti-HCV subtype 1b NS5B抗体[10D6] (ab100895)
Key features and details
- Mouse monoclonal [10D6] to HCV subtype 1b NS5B
- Suitable for: WB, IP, ELISA
- Reacts with: Hepatitis C virus
- Isotype: IgG1
概述
-
产品名称
Anti-HCV subtype 1b NS5B抗体[10D6]
参阅全部 HCV subtype 1b NS5B 一抗 -
描述
小鼠单克隆抗体[10D6] to HCV subtype 1b NS5B -
宿主
Mouse -
经测试应用
适用于: WB, IP, ELISAmore details -
种属反应性
与反应: Hepatitis C virus -
免疫原
Recombinant fragment:
LSSKAVNHIRSVWKDLLEDT
, corresponding to amino acids 111-130 of HCV subtype 1b NS5B -
阳性对照
- Huh7 cells harboring selectable subgenomic HCV RNA replicon
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
-
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
10D6 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab100895于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/1000. Predicted molecular weight: 65 kDa.
|
IP |
Use at an assay dependent dilution.
|
|
ELISA |
1/1000.
|
说明 |
---|
WB
1/1000. Predicted molecular weight: 65 kDa. |
IP
Use at an assay dependent dilution. |
ELISA
1/1000. |
靶标
-
细胞定位
Host cytoplasm, nucleus, mitochondrion, endoplasmatic reticulum; secreted. -
数据库链接
- SwissProt: Q9WMX2 Hepatitis C virus
-
别名
- Hepacivirin antibody
- NS3P antibody
- POLG_HCVBK antibody
- Serine protease NS3 antibody
图片
-
IP was carried out with ab100895 using the lysates of Huh7 cells harboring selectable subgenomic HCV RNA replicon (upper panel) or plain Huh7 cells (lower panel). NS5B polyclonal antibodies (-NS5B) and -Bcl2 mAb, directed against cellular protein, were used as positive and negative controls respectively. Asterisk indicates immunoglobulin heavy chain; protein blots were probed with ab100895.
-
All lanes : Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
Lane 1 : Huh-7
transfected with HCV replicon
Lane 2 : Huh-7 nontransfected
cells
Predicted band size: 65 kDa
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab100895 被引用在 2 文献中.
- Chen SR et al. In Vitro Study on Anti-Hepatitis C Virus Activity of Spatholobus suberectus Dunn. Molecules 21:N/A (2016). PubMed: 27754461
- Vogt DA et al. Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. PLoS Pathog 9:e1003302 (2013). WB . PubMed: 23593007