Anti-HCN1抗体- C-terminal (ab229340)
Key features and details
- Rabbit polyclonal to HCN1 - C-terminal
- Suitable for: IHC-Fr, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-HCN1抗体- C-terminal
参阅全部 HCN1 一抗 -
描述
兔多克隆抗体to HCN1 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human HCN1 (C terminal). The exact sequence is proprietary.
Database link: O60741 -
阳性对照
- WB: Mouse and rat brain tissue extracts; SK-N-AS whole cell extract. IHC-P: Mouse retina and brain tissues; rat brain tissue. ICC/IF: Rat DIV9 E18 primary cortical neurons. IHC-Fr: Frozen mouse cerebellum and hippocampus tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab229340于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 99 kDa.
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说明 |
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IHC-Fr
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 99 kDa. |
靶标
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功能
Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli. -
组织特异性
Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas. -
序列相似性
Belongs to the potassium channel HCN family.
Contains 1 cyclic nucleotide-binding domain. -
结构域
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 348980 Human
- Entrez Gene: 15165 Mouse
- Entrez Gene: 84390 Rat
- Omim: 602780 Human
- SwissProt: O60741 Human
- SwissProt: O88704 Mouse
- SwissProt: Q9JKB0 Rat
- Unigene: 650434 Human
see all -
别名
- BCNG-1 antibody
- BCNG1 antibody
- Brain cyclic nucleotide gated channel 1 antibody
see all
图片
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Anti-HCN1 antibody - C-terminal (ab229340) at 1/1000 dilution + SK-N-AS (human neuroblastoma cell line) whole cell extract at 30 µg
Predicted band size: 99 kDa7.5% SDS-PAGE gel.
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4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons cells stained for HCN1 (green) using ab229340 at 1/500 dilution in ICC/IF.
Beta Tubulin 3/ Tuj1 (red).
Nuclear counterstain: Fluoroshield with DAPI (blue).
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Paraffin-embedded rat brain tissue stained for HCN1 using ab229340 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse brain tissue stained for HCN1 using ab229340 at 1/500 dilution in immunohistochemical analysis.
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Frozen-sectioned adult mouse hippocampus tissue stained for HCN1 (green) using ab229340 at 1/250 dilution in immunohistochemical analysis.
Nuclear counterstain: Fluoroshield with DAPI (blue)
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All lanes : Anti-HCN1 antibody - C-terminal (ab229340) at 1/1000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 99 kDa7.5% SDS-PAGE gel.
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Frozen-sectioned adult mouse cerebellum tissue stained for HCN1 (green) using ab229340 at 1/250 dilution in immunohistochemical analysis.
beta Tubulin 3/ TUJ1 (red).
Nuclear counterstain: Fluoroshield with DAPI (blue).
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Paraffin-embedded mouse retina tissue stained for HCN1 using ab229340 at 1/800 dilution in immunohistochemical abalysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab229340 被引用在 3 文献中.
- Zhong W & Darmani NA The HCN Channel Blocker ZD7288 Induces Emesis in the Least Shrew (Cryptotis parva). Front Pharmacol 12:647021 (2021). PubMed: 33995059
- Hughes BA et al. Chronic ethanol exposure alters prelimbic prefrontal cortical Fast-Spiking and Martinotti interneuron function with differential sex specificity in rat brain. Neuropharmacology 162:107805 (2020). PubMed: 31589884
- Kashyap M et al. Constitutively active HCN channels constrain detrusor excitability and modulate evoked contractions of human bladder. Am J Clin Exp Urol 8:163-176 (2020). PubMed: 33235894