Anti-hCG抗体[HCG1] (ab400)
Key features and details
- Mouse monoclonal [HCG1] to hCG
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-hCG抗体[HCG1]
参阅全部 hCG 一抗 -
描述
小鼠单克隆抗体[HCG1] to hCG -
宿主
Mouse -
特异性
hCG specifically recognises human chorionic gonadotropin. Its affinity for the free beta-chain is slightly greater than its affinity for the whole molecule. hCG - 100% hCG (beta) - 125% hCG (alpha) - <1% hLH - <1% hTSH - <0.05% hFSH - <<0.1% Affinity constant: 5x109 l/mol HCG. -
种属反应性
与反应: Human -
常规说明
Association Constant: hCG: 4.0 x 106 and hCGbeta: 1.6 x 106
Dissociation Constant: hCG: 1.4 x 10-4 and hCGbeta: 3.6 x 10-4
Affinity Constant hCG: KA = 2.9 x 1010 1/M; KD = 3.4 x 10-11 M (= 0.03 nM)
Affinity Constant hCGbeta: KA = 4.4 x 109 1/M; KD = 2.3 x 10-10 M (= 0.23 nM)
Determination method: SPR analysis
Antigen: hCG; hCG-betaThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: 0.9% Sodium chloride -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
HCG1 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
靶标
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相关性
Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma. -
细胞定位
Secreted -
数据库链接
- Entrez Gene: 1081 Human
- Entrez Gene: 1082 Human
- Omim: 118850 Human
- Omim: 118860 Human
- SwissProt: P01215 Human
- SwissProt: P01233 Human
- Unigene: 119689 Human
- Unigene: 446683 Human
see all -
别名
- anterior pituitary glycoprotein hormones common subunit alpha antibody
- CG alpha antibody
- CG-ALPHA antibody
see all
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab400 被引用在 5 文献中.
- Haslam C et al. Label-Free Sensors Based on Graphene Field-Effect Transistors for the Detection of Human Chorionic Gonadotropin Cancer Risk Biomarker. Diagnostics (Basel) 8:N/A (2018). PubMed: 29316718
- Xu RH et al. BMP4 initiates human embryonic stem cell differentiation to trophoblast. Nat Biotechnol 20:1261-4 (2002). PubMed: 12426580
- Birnbaum S et al. Latex-based thin-layer immunoaffinity chromatography for quantitation of protein analytes. Anal Biochem 206:168-71 (1992). PubMed: 1456429
- Pettersson K et al. Time-resolved fluoroimmunoassay of human choriogonadotropin. Clin Chem 29:60-4 (1983). PubMed: 6848283
- Wahlstrom T et al. The use of monoclonal antibodies against human chorionic gonadotropin for immunoperoxidase staining of normal placenta, pituitary gland, and pituitary adenomas. J Histochem Cytochem 29:864-5 (1981). PubMed: 6167612