Anti-hCG抗体[HCG1] (ab400)

Reviews (1)Q&A (10)References (5)

Key features and details

  • Mouse monoclonal [HCG1] to hCG
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-hCG抗体[HCG1]
    参阅全部 hCG 一抗

  • 描述

    小鼠单克隆抗体[HCG1] to hCG

  • 宿主

    Mouse

  • 特异性

    hCG specifically recognises human chorionic gonadotropin. Its affinity for the free beta-chain is slightly greater than its affinity for the whole molecule. hCG - 100% hCG (beta) - 125% hCG (alpha) - <1% hLH - <1% hTSH - <0.05% hFSH - <<0.1% Affinity constant: 5x109 l/mol HCG.

  • 种属反应性

    与反应: Human

  • 常规说明

    Association Constant: hCG: 4.0 x 106 and hCGbeta: 1.6 x 106
    Dissociation Constant: hCG: 1.4 x 10-4 and hCGbeta: 3.6 x 10-4
    Affinity Constant hCG: KA = 2.9 x 1010 1/M; KD = 3.4 x 10-11 M (= 0.03 nM)
    Affinity Constant hCGbeta: KA = 4.4 x 109 1/M; KD = 2.3 x 10-10 M (= 0.23 nM)
    Determination method: SPR analysis
    Antigen: hCG; hCG-beta

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

靶标

  • 相关性

    Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma.

  • 细胞定位

    Secreted

  • 数据库链接

    see all

  • 别名

    • anterior pituitary glycoprotein hormones common subunit alpha antibody
    • CG alpha antibody
    • CG-ALPHA antibody
    • CGB antibody
    • CGB3 antibody
    • CGB5 antibody
    • CGB7 antibody
    • choriogonadotropin alpha chain antibody
    • Chorionic gonadotrophin chain beta antibody
    • chorionic gonadotrophin subunit alpha antibody
    • chorionic gonadotropin beta 3 subunit antibody
    • Chorionic gonadotropin beta chain antibody
    • Chorionic gonadotropin beta subunit antibody
    • chorionic gonadotropin, alpha polypeptide antibody
    • chorionic gonadotropin, beta polypeptide 3 antibody
    • chorionic gonadotropin, beta polypeptide antibody
    • Follicle stimulating hormone alpha chain antibody
    • follicle-stimulating hormone alpha chain antibody
    • follicle-stimulating hormone alpha subunit antibody
    • follitropin alpha chain antibody
    • FSH Alpha antibody
    • FSH-alpha antibody
    • FSHA antibody
    • glycoprotein hormones alpha chain antibody
    • glycoprotein hormones, alpha polypeptide antibody
    • GPHa antibody
    • GPHA1 antibody
    • HCG antibody
    • hCGB antibody
    • Human Chorionic Gonadotropin antibody
    • LHA antibody
    • LSH alpha antibody
    • LSH-alpha antibody
    • luteinizing hormone alpha chain antibody
    • lutropin alpha chain antibody
    • Thyroid stimulating hormone alpha chain antibody
    • thyroid-stimulating hormone alpha chain antibody
    • thyrotropin alpha chain antibody
    • TSH Alpha antibody
    • TSH-alpha antibody
    • TSHA antibody
    see all

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (5)

发表研究结果有使用 ab400？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab400 被引用在 5 文献中.

  • Haslam C  et al. Label-Free Sensors Based on Graphene Field-Effect Transistors for the Detection of Human Chorionic Gonadotropin Cancer Risk Biomarker. Diagnostics (Basel) 8:N/A (2018). PubMed: 29316718
  • Xu RH  et al. BMP4 initiates human embryonic stem cell differentiation to trophoblast. Nat Biotechnol 20:1261-4 (2002). PubMed: 12426580
  • Birnbaum S  et al. Latex-based thin-layer immunoaffinity chromatography for quantitation of protein analytes. Anal Biochem 206:168-71 (1992). PubMed: 1456429
  • Pettersson K  et al. Time-resolved fluoroimmunoassay of human choriogonadotropin. Clin Chem 29:60-4 (1983). PubMed: 6848283
  • Wahlstrom T  et al. The use of monoclonal antibodies against human chorionic gonadotropin for immunoperoxidase staining of normal placenta, pituitary gland, and pituitary adenomas. J Histochem Cytochem 29:864-5 (1981). PubMed: 6167612

客户评价及客户问答

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1-10 of 11 Abreviews or Q&A

Question

What hCG-related molecules(hCG consisted of Beta and alfa subunit, hCG-Beta, Nicked hCG, Nicked hCG-Beta, Nicked hCG missing Beta subunit C-terminal peptide, Hyperglycosylated hCG) can this product specificly bind with?

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Answer

This clone has been reported to detect intact hCG, hyperglycosylated hCG, nicked hCG, hCGb and nicked hCGb.

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Question

Could you send the reference for specific binding between ab400 and ab126653 in general notes of antibody datasheet for me?
I am worried that it cannot bind together.

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Answer

The antibody ab400 was tested for specific binding to hCG, but not the hCG we supply as ab126653. There is no published journal reference for the testing of the antibody ab400, and I am fairly certain there is no commercial source of the hCG protein that was used for the testing. However, I am confident that ab400 will bind to ab126653.

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Question

I would like to know whether ab400 can bind ab126653, yes or not ?

In the case of ab126653 cannot bind to ab400, please recommend other BhCG protein that can definitely bind to ab400 antibody.

The application used is SPR/ELISA.

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Answer

We have not tested the two products together, ab400 antibody and ab126653 protein, but we assume that ab126653 is almost identical, if not exactly identical, to the hCG beta that was used to determine the binding constants listed in the general notes section of the antibody datasheet.

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Question

Many thanks for your email.

I need a monoclonal antibody anti-hCG, for direct ELISA. Preferably mouse and hCG-beta.

I've been using your antibody ab400 for indirect ELISA, and quite I'm happy with the results.
But at the moment, I need an antibody HRP labeled.

Just let me know whether do you have a product that may fit on my request.

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Answer

Thank you for your reply.

Just one more thing for clarification:

With "mouse", do you mean it should be mouse monoclonal or against the species mouse?

Sorry for the delay.

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Question

I would like to know whether do you have available or can produce ab400 HRP conjugated.

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Answer

Thank you for contacting us.

Unfortunately, we do not sell this antibody in the conjugated form nor do we offer a conjugation service. However, we do have a conjugation kit available.

ab102891 EasyLink HRP Conjugation Kit (3 x 100µg HRP):
https://www.abcam.com/EasyLink-HRP-Conjugation-Kit-3-x-100g-HRP-ab102891.html https://www.abcam.com/EasyLink-HRP-Conjugation-Kit-3-x-100g-HRP-ab102891.html.

In addition, as the antibody contains sodium azide which interferes with this process and inhibits the HRP a purification step is necessary:

ab102783 Antibody Purification kit (1 purification)
https://www.abcam.com/Antibody-Purification-kit-1-purification-ab102783.html https://www.abcam.com/Antibody-Purification-kit-1-purification-ab102783.html.

Otherwise, if you let me know the target species and the application you want to move I can check our other antibodies for their suitability.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



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Question

To whom it may concern

I would like to know the pI (isoelectric point) value of the anti-hCG antibody (ab400).
I would also like to know the pH of the storage buffer.

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Answer

Thank you for your patience.

Sorry for the late response. The specific pI range of ab400 is 6.4-7.9. However, we are sorry that we have not determined the pH of the storage buffer.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

To whom it may concern,   We have recently purchased Ab400 and ab9071  which come in liquid form. Could you please tell  us the buffer composition?

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Answer

Thank you for contacting us.





AB400 is shipped in a liquid form in which the antibody is suspended in a solution containing 0.9% Sodium Chloride and0.1% Sodium Azide (as a preservative).

AB9071is shipped in a liquid form in which the antibody is suspended in PBS.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I recently purchased some Anti-hCG antibody [HCG1] (ab400). I was wondering if you have any measured values for the rate constants, either Kon or Koff for this antibody and hCG? Thanks,

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Answer

Thank you for contacting us and sorry for the delay of our reply. The source of the anti-hCG ab400 sent us the following kinetic parameters for this product : Association constants : whole hCG : 4.0 x 10^6 hCG (beta-subunit) : 1.6 x 10^6 Dissociation constants : whole hCG : 1.4 x 10^-4 hCG (beta-subunit) : 3.6 x 10^-4 Affinity constant whole hCG : KA = 2.9 x 10^10 1/M KD = 3.4 x 10^-11 M (= 0.034 nM) Affinity constant hCG (beta-subunit) : KA = 4.4 x 10^9 1/M KD = 2.3 x 10^-10 M (= 0.23 nM) Determination method : SPR analysis Cross-reactivities : hCG alpha < 1 % LH < 1 % TSH < 0.05 % FSH < 0.1 % I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Western blot abreview for Anti-hCG antibody [HCG1]

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Abreviews
Application
Western blot
Sample
Human Purified protein (Pregnancy hCG)
Loading amount
1000 µg
Specification
Pregnancy hCG
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

提交于 Sep 21 2007

Question

Is there more information about how this antibody was tested in IF? Is there an image available?

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Answer

Thank you for your enquiry and patience. I regret to inform you that this antibody has not been tested for application in IF and I have updated the online datasheet to reflect this. If you have any additional questions, please contact us again.

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