Anti-hCG抗体[HCG1] (ab400)

Reviews (1)Q&A (10)References (8)

Key features and details

  • Mouse monoclonal [HCG1] to hCG
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-hCG抗体[HCG1]
    参阅全部 hCG 一抗

  • 描述

    小鼠单克隆抗体[HCG1] to hCG

  • 宿主

    Mouse

  • 特异性

    hCG specifically recognises human chorionic gonadotropin. Its affinity for the free beta-chain is slightly greater than its affinity for the whole molecule. hCG - 100% hCG (beta) - 125% hCG (alpha) - <1% hLH - <1% hTSH - <0.05% hFSH - <<0.1% Affinity constant: 5x109 l/mol HCG.

  • 种属反应性

    与反应: Human

  • 常规说明

    Association Constant: hCG: 4.0 x 106 and hCGbeta: 1.6 x 106
    Dissociation Constant: hCG: 1.4 x 10-4 and hCGbeta: 3.6 x 10-4
    Affinity Constant hCG: KA = 2.9 x 1010 1/M; KD = 3.4 x 10-11 M (= 0.03 nM)
    Affinity Constant hCGbeta: KA = 4.4 x 109 1/M; KD = 2.3 x 10-10 M (= 0.23 nM)
    Determination method: SPR analysis
    Antigen: hCG; hCG-beta

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

靶标

  • 相关性

    Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma.

  • 细胞定位

    Secreted

  • 数据库链接

    • 别名

      • anterior pituitary glycoprotein hormones common subunit alpha antibody
      • CG alpha antibody
      • CG-ALPHA antibody
      • CGB antibody
      • CGB3 antibody
      • CGB5 antibody
      • CGB7 antibody
      • choriogonadotropin alpha chain antibody
      • Chorionic gonadotrophin chain beta antibody
      • chorionic gonadotrophin subunit alpha antibody
      • chorionic gonadotropin beta 3 subunit antibody
      • Chorionic gonadotropin beta chain antibody
      • Chorionic gonadotropin beta subunit antibody
      • chorionic gonadotropin, alpha polypeptide antibody
      • chorionic gonadotropin, beta polypeptide 3 antibody
      • chorionic gonadotropin, beta polypeptide antibody
      • Follicle stimulating hormone alpha chain antibody
      • follicle-stimulating hormone alpha chain antibody
      • follicle-stimulating hormone alpha subunit antibody
      • follitropin alpha chain antibody
      • FSH Alpha antibody
      • FSH-alpha antibody
      • FSHA antibody
      • glycoprotein hormones alpha chain antibody
      • glycoprotein hormones, alpha polypeptide antibody
      • GPHa antibody
      • GPHA1 antibody
      • HCG antibody
      • hCGB antibody
      • Human Chorionic Gonadotropin antibody
      • LHA antibody
      • LSH alpha antibody
      • LSH-alpha antibody
      • luteinizing hormone alpha chain antibody
      • lutropin alpha chain antibody
      • Thyroid stimulating hormone alpha chain antibody
      • thyroid-stimulating hormone alpha chain antibody
      • thyrotropin alpha chain antibody
      • TSH Alpha antibody
      • TSH-alpha antibody
      • TSHA antibody
      see all

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (8)

    发表研究结果有使用 ab400？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab400 被引用在 8 文献中.

    • Wang G  et al. An engineered oncolytic virus expressing PD-L1 inhibitors activates tumor neoantigen-specific T cell responses. Nat Commun 11:1395 (2020). PubMed: 32170083
    • Hong G  et al. New photothermal immunoassay of human chorionic gonadotropin using Prussian blue nanoparticle-based photothermal conversion. Anal Bioanal Chem 411:6837-6845 (2019). PubMed: 31471682
    • Fang S  et al. Anti-CD47 antibody eliminates bone tumors in rats. Saudi J Biol Sci 26:2074-2078 (2019). PubMed: 31889797
    • Haslam C  et al. Label-Free Sensors Based on Graphene Field-Effect Transistors for the Detection of Human Chorionic Gonadotropin Cancer Risk Biomarker. Diagnostics (Basel) 8:N/A (2018). PubMed: 29316718
    • Xu RH  et al. BMP4 initiates human embryonic stem cell differentiation to trophoblast. Nat Biotechnol 20:1261-4 (2002). PubMed: 12426580
    • Birnbaum S  et al. Latex-based thin-layer immunoaffinity chromatography for quantitation of protein analytes. Anal Biochem 206:168-71 (1992). PubMed: 1456429
    • Pettersson K  et al. Time-resolved fluoroimmunoassay of human choriogonadotropin. Clin Chem 29:60-4 (1983). PubMed: 6848283
    • Wahlstrom T  et al. The use of monoclonal antibodies against human chorionic gonadotropin for immunoperoxidase staining of normal placenta, pituitary gland, and pituitary adenomas. J Histochem Cytochem 29:864-5 (1981). PubMed: 6167612

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    1-10 of 11 Abreviews or Q&A

    Western blot abreview for Anti-hCG antibody [HCG1]

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Human Purified protein (Pregnancy hCG)
    Loading amount
    1000 µg
    Specification
    Pregnancy hCG
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
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    Abcam user community

    Verified customer

    提交于 Sep 21 2007

    Question

    What hCG-related molecules(hCG consisted of Beta and alfa subunit, hCG-Beta, Nicked hCG, Nicked hCG-Beta, Nicked hCG missing Beta subunit C-terminal peptide, Hyperglycosylated hCG) can this product specificly bind with?

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    Answer

    This clone has been reported to detect intact hCG, hyperglycosylated hCG, nicked hCG, hCGb and nicked hCGb.

    Read More

    Question

    Could you send the reference for specific binding between ab400 and ab126653 in general notes of antibody datasheet for me?
    I am worried that it cannot bind together.

    Read More
    Answer

    The antibody ab400 was tested for specific binding to hCG, but not the hCG we supply as ab126653. There is no published journal reference for the testing of the antibody ab400, and I am fairly certain there is no commercial source of the hCG protein that was used for the testing. However, I am confident that ab400 will bind to ab126653.

    Read More

    Question

    I would like to know whether ab400 can bind ab126653, yes or not ?

    In the case of ab126653 cannot bind to ab400, please recommend other BhCG protein that can definitely bind to ab400 antibody.

    The application used is SPR/ELISA.

    Read More
    Answer

    We have not tested the two products together, ab400 antibody and ab126653 protein, but we assume that ab126653 is almost identical, if not exactly identical, to the hCG beta that was used to determine the binding constants listed in the general notes section of the antibody datasheet.

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    Question

    Many thanks for your email.

    I need a monoclonal antibody anti-hCG, for direct ELISA. Preferably mouse and hCG-beta.

    I've been using your antibody ab400 for indirect ELISA, and quite I'm happy with the results.
    But at the moment, I need an antibody HRP labeled.

    Just let me know whether do you have a product that may fit on my request.

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    Answer

    Thank you for your reply.

    Just one more thing for clarification:

    With "mouse", do you mean it should be mouse monoclonal or against the species mouse?

    Sorry for the delay.

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    Question

    I would like to know whether do you have available or can produce ab400 HRP conjugated.

    Read More
    Answer

    Thank you for contacting us.

    Unfortunately, we do not sell this antibody in the conjugated form nor do we offer a conjugation service. However, we do have a conjugation kit available.

    ab102891 EasyLink HRP Conjugation Kit (3 x 100µg HRP):
    https://www.abcam.com/EasyLink-HRP-Conjugation-Kit-3-x-100g-HRP-ab102891.html https://www.abcam.com/EasyLink-HRP-Conjugation-Kit-3-x-100g-HRP-ab102891.html.

    In addition, as the antibody contains sodium azide which interferes with this process and inhibits the HRP a purification step is necessary:

    ab102783 Antibody Purification kit (1 purification)
    https://www.abcam.com/Antibody-Purification-kit-1-purification-ab102783.html https://www.abcam.com/Antibody-Purification-kit-1-purification-ab102783.html.

    Otherwise, if you let me know the target species and the application you want to move I can check our other antibodies for their suitability.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



    Use our products? Submit an Abreview. Earn rewards!

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    Question

    To whom it may concern

    I would like to know the pI (isoelectric point) value of the anti-hCG antibody (ab400).
    I would also like to know the pH of the storage buffer.

    Read More
    Answer

    Thank you for your patience.

    Sorry for the late response. The specific pI range of ab400 is 6.4-7.9. However, we are sorry that we have not determined the pH of the storage buffer.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    To whom it may concern,   We have recently purchased Ab400 and ab9071  which come in liquid form. Could you please tell  us the buffer composition?

    Read More
    Answer

    Thank you for contacting us.





    AB400 is shipped in a liquid form in which the antibody is suspended in a solution containing 0.9% Sodium Chloride and0.1% Sodium Azide (as a preservative).

    AB9071is shipped in a liquid form in which the antibody is suspended in PBS.



    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    I recently purchased some Anti-hCG antibody [HCG1] (ab400). I was wondering if you have any measured values for the rate constants, either Kon or Koff for this antibody and hCG? Thanks,

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    Answer

    Thank you for contacting us and sorry for the delay of our reply. The source of the anti-hCG ab400 sent us the following kinetic parameters for this product : Association constants : whole hCG : 4.0 x 10^6 hCG (beta-subunit) : 1.6 x 10^6 Dissociation constants : whole hCG : 1.4 x 10^-4 hCG (beta-subunit) : 3.6 x 10^-4 Affinity constant whole hCG : KA = 2.9 x 10^10 1/M KD = 3.4 x 10^-11 M (= 0.034 nM) Affinity constant hCG (beta-subunit) : KA = 4.4 x 10^9 1/M KD = 2.3 x 10^-10 M (= 0.23 nM) Determination method : SPR analysis Cross-reactivities : hCG alpha < 1 % LH < 1 % TSH < 0.05 % FSH < 0.1 % I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    Is there more information about how this antibody was tested in IF? Is there an image available?

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    Answer

    Thank you for your enquiry and patience. I regret to inform you that this antibody has not been tested for application in IF and I have updated the online datasheet to reflect this. If you have any additional questions, please contact us again.

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    1-10 of 11 Abreviews or Q&A

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