Anti-HAUSP / USP7抗体(ab4080)
Key features and details
- Rabbit polyclonal to HAUSP / USP7
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-HAUSP / USP7抗体
参阅全部 HAUSP / USP7 一抗 -
描述
兔多克隆抗体to HAUSP / USP7 -
宿主
Rabbit -
特异性
By Western blot, this antibody detects a single clean band of about 130kD representing HAUSP in various cell lysates (see picture). The band is strongly depleted using peptide blocking with the immunising peptide. -
经测试应用
适用于: ELISA, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide:
TDDPPRITQNPVC
, corresponding to amino acids 30-41 of Human HAUSP. -
常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab4080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | 1/15000 - 1/60000. | |
| WB | 1/500 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 128 kDa). | |
| ICC/IF | Use a concentration of 1 µg/ml. |
靶标
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功能
Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN and DAXX (PubMed:11923872, PubMed:15053880, PubMed:16964248, PubMed:18716620, PubMed:25283148). Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis (PubMed:25283148). Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage: recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6. Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection. Involved in maintenance of DNA methylation via its interaction with UHRF1 and DNMT1: acts by mediating deubiquitination of UHRF1 and DNMT1, preventing their degradation and promoting DNA methylation by DNMT1 (PubMed:21745816). Exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Increases regulatory T-cells (Treg) suppressive capacity by deubiquitinating and stabilizing the transcription factor FOXP3 which is crucial for Treg cell function (PubMed:23973222). -
组织特异性
Widely expressed. Overexpressed in prostate cancer. -
序列相似性
Belongs to the peptidase C19 family.
Contains 1 MATH domain.
Contains 1 USP domain. -
结构域
The C-terminus plays a role in its oligomerization. -
翻译后修饰
Isoform 1: Phosphorylated. Isoform 1 is phosphorylated at positions Ser-18 and Ser-963. Isoform 2: Not phosphorylated.
Isoform 1: Polyneddylated. Isoform 2: Not Polyneddylated.
Isoform 1 and isoform 2: Not sumoylated.
Isoform 1 and isoform 2: Polyubiquitinated by herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110; leading to its subsequent proteasomal degradation. Isoform 1: Ubiquitinated at Lys-869. -
细胞定位
Nucleus. Cytoplasm. Nucleus, PML body. Present in a minority of ND10 nuclear bodies. Association with ICP0/VMW110 at early times of infection leads to an increased proportion of USP7-containing ND10. Colocalizes with ATXN1 in the nucleus. Colocalized with DAXX in speckled structures. Colocalized with PML and PTEN in promyelocytic leukemia protein (PML) nuclear bodies. - Information by UniProt
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数据库链接
- Entrez Gene: 7874 Human
- Omim: 602519 Human
- SwissProt: Q93009 Human
- Unigene: 386939 Human
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别名
- Deubiquitinating enzyme 7 antibody
- HAUSP antibody
- Herpes virus associated ubiquitin specific protease antibody
see all
图片
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Western blot - Anti-HAUSP / USP7 antibody (ab4080)
Western blot using ab4080 at 1/500. Secondary antibody anti-rabbit HRP ab6721 at 1/5000. Exposure time 30s.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: MCF7
Lane 5: 3T3
Lane 6: Hela nuclear + ab11707 HAUSP peptide (aa30-41)
Lane 7: Hela + ab11707 HAUSP peptide (aa30-41)
Lane 8: A431 + ab11707 HAUSP peptide (aa30-41)
Lane 9: MCF7 + ab11707 HAUSP peptide (aa30-41)
Lane 10: 3T3 + ab11707 HAUSP peptide (aa30-41)Western blot using ab4080 at 1/500. Secondary ab6721 at 1/5000. Exposure time 30s.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: MCF7
Lane 5: 3T3
Lane 6: Hela nuclear + ab11707 HAUSP peptide (aa30-41)
Lane 7: Hela + ab11707 HAUSP peptide (aa30-41)
Lane 8: A431 + ab11707 HAUSP peptide (aa30-41)
Lane 9: MCF7 + ab11707 HAUSP peptide (aa30-41)
Lane 10: 3T3 + ab11707 HAUSP peptide (aa30-41) -
Immunocytochemistry/ Immunofluorescence - Anti-HAUSP / USP7 antibody (ab4080)ICC/IF image of ab4080 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4080, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
ELISA - Anti-HAUSP / USP7 antibody (ab4080)ELISA results of purified ab4080 tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Peroxidase Conjugated secondary antibody and TMB substrate.
实验方案
数据表及文件
文献 (18)
ab4080 被引用在 18 文献中.
- Carias KV et al. A MAGEL2-deubiquitinase complex modulates the ubiquitination of circadian rhythm protein CRY1. PLoS One 15:e0230874 (2020). PubMed: 32315313
- Zeng Q et al. Ubiquitin-specific protease 7 promotes osteosarcoma cell metastasis by inducing epithelial-mesenchymal transition. Oncol Rep 41:543-551 (2019). PubMed: 30542736
- Masuda Y et al. Preferential digestion of PCNA-ubiquitin and p53-ubiquitin linkages by USP7 to remove polyubiquitin chains from substrates. J Biol Chem 294:4177-4187 (2019). PubMed: 30647135
- Biswas K et al. BRE/BRCC45 regulates CDC25A stability by recruiting USP7 in response to DNA damage. Nat Commun 9:537 (2018). PubMed: 29416040
- Kapadia B et al. Fatty Acid Synthase induced S6Kinase facilitates USP11-eIF4B complex formation for sustained oncogenic translation in DLBCL. Nat Commun 9:829 (2018). PubMed: 29483509
