Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (Gradient Gel)
Sample
Human Cell lysate - whole cell (Different Breast Cancer Lines - MDAMB231 453 Cal12)
Specification
Different Breast Cancer Lines - MDAMB231 453 Cal12
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.5% Tween + Azide
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Mouse
Clonality: Polyclonal
Conjugation: Other
Host species: Mouse
Clonality: Polyclonal
Conjugation: Other
Dilution
1/5000
Detection
Detection method
Odyssey machine
Bands
Specific: 145 kDa
Exposure
1 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Dec 23 2013