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Anti-Hairy Cell Leukemia抗体[DBA.44]，prediluted (ab857)

Submit a review Q&A (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted (ab857)

    Key features and details

    • Mouse monoclonal [DBA.44] to Hairy Cell Leukemia, prediluted
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgM

    概述

    • 产品名称

      Anti-Hairy Cell Leukemia抗体[DBA.44]，prediluted
      参阅全部 Hairy Cell Leukemia 一抗

    • 描述

      小鼠单克隆抗体[DBA.44] to Hairy Cell Leukemia，prediluted

    • 宿主

      Mouse

    • 特异性

      This antibody reacts with over 97% of hairy cell leukaemias as well as about 35% of high grade B cell lymphomas. It reacts with B lymphocytes, cells of mantle zone and immunoblasts outside the lymphoid tissues.

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Tissue, cells or virus corresponding to Hairy Cell Leukemia. BALB/C mice were injected with the Deau cell line established from a large cell lymphoma.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C. Do Not Freeze.

    • 存储溶液

      pH: 7.3
      Preservative: 0.05% Sodium azide

      Inert stabilizer
    • Concentration information loading...

    • 克隆

      单克隆

    • 克隆编号

      DBA.44

    • 骨髓瘤

      unknown

    • 同种型

      IgM

    • 轻链类型

      kappa

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab857于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    Use at an assay dependent concentration.
    说明
    IHC-P
    Use at an assay dependent concentration.

    靶标

    • 细胞定位

      Nuclear

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted (ab857)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted (ab857)

      Immunohistochemistry (Formalin fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Hairy Cell Leukemia with ab857.

    文献 (0)

    发表研究结果有使用 ab857？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab857 尚未被引用在任何文献中。

    客户评价及客户问答

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    Question

    BATCH NUMBER 143106 ORDER NUMBER 105179 DESCRIPTION OF THE PROBLEM No staining SAMPLE Species PRIMARY ANTIBODY DBA.44 for 1hr at RT DETECTION METHOD DAB+ POSITIVE AND NEGATIVE CONTROLS USED Spleen with Hairy cell leukemia ANTIBODY STORAGE CONDITIONS Antibody was received 7 days after shipment due to US customs hold. Antibody was received hot! FIXATION OF SAMPLE Formalin ANTIGEN RETRIEVAL Enzymatic using DAKO proteolytic enzyme for 5 minutes at room temperature. BLOCKING CONDITIONS H2O2 SECONDARY ANTIBODY DAKO LSAB2 HRP HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

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    Answer

    Thank you for contacting us for technical support, I'm very sorry to hear you are experiencing problems with ab857 and it does appear that the delayed shipment of the antibody damaged it as your protocol looks very good (you still may need to increase the incubation time to overnight depending on the tissue type). I can arrange a replacement vial or offer you a refund if you would prefer, could you please let me know what you prefer and I will arrange this immediately? I look forward to hearing from you,

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