Anti-Hairy Cell Leukemia抗体[DBA.44]，prediluted (ab857)

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Key features and details

  • Mouse monoclonal [DBA.44] to Hairy Cell Leukemia, prediluted
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Human
  • Isotype: IgM

概述

  • 产品名称

    Anti-Hairy Cell Leukemia抗体[DBA.44]，prediluted
    参阅全部 Hairy Cell Leukemia 一抗

  • 描述

    小鼠单克隆抗体[DBA.44] to Hairy Cell Leukemia，prediluted

  • 宿主

    Mouse

  • 特异性

    This antibody reacts with over 97% of hairy cell leukaemias as well as about 35% of high grade B cell lymphomas. It reacts with B lymphocytes, cells of mantle zone and immunoblasts outside the lymphoid tissues.

  • 经测试应用

    适用于: IHC-P, IHC-Frmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    BALB/C mice were injected with the Deau cell line established from a large cell lymphoma.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • 存储溶液

    Preservative: 0.05% Sodium azide

    Inert stabilizer
  • Concentration information loading...

  • 克隆

    单克隆

  • 克隆编号

    DBA.44

  • 骨髓瘤

    unknown

  • 同种型

    IgM

  • 轻链类型

    kappa

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent dilution.

靶标

  • 细胞定位

    Nuclear

文献 (0)

发表研究结果有使用 ab857？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab857 尚未被引用在任何文献中。

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Question

BATCH NUMBER 143106 ORDER NUMBER 105179 DESCRIPTION OF THE PROBLEM No staining SAMPLE Species PRIMARY ANTIBODY DBA.44 for 1hr at RT DETECTION METHOD DAB+ POSITIVE AND NEGATIVE CONTROLS USED Spleen with Hairy cell leukemia ANTIBODY STORAGE CONDITIONS Antibody was received 7 days after shipment due to US customs hold. Antibody was received hot! FIXATION OF SAMPLE Formalin ANTIGEN RETRIEVAL Enzymatic using DAKO proteolytic enzyme for 5 minutes at room temperature. BLOCKING CONDITIONS H2O2 SECONDARY ANTIBODY DAKO LSAB2 HRP HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

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Answer

Thank you for contacting us for technical support, I'm very sorry to hear you are experiencing problems with ab857 and it does appear that the delayed shipment of the antibody damaged it as your protocol looks very good (you still may need to increase the incubation time to overnight depending on the tissue type). I can arrange a replacement vial or offer you a refund if you would prefer, could you please let me know what you prefer and I will arrange this immediately? I look forward to hearing from you,

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