Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% tritonx100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/1000
Additional data
Additional Notes
HeLa cells were transfected with a HA-Tag protein, and 72 hour post transfection were fixed, permeablized, blocked and stained for HA Tag using ab18181. Gaot anti-mouse FITC tagged antibody was used as secondary antibody
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Harendra Chahar
Verified customer
提交于 Sep 10 2012