概述

  • 产品名称
    Anti-HA tag抗体(FITC)
    参阅全部 HA tag 一抗
  • 描述
    兔多克隆抗体to HA tag (FITC)
  • 宿主
    Rabbit
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 免疫原

    YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH.

  • 常规说明

    Molar F/P ratio is 3.7:1.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: 0.2% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • 别名
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

图片

  • ab1208 staining Ha tag in human breast cancer cells (HA tagged expression plasmid) by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.2% Triton X-100 in PBS and then blocked using 1% BSA and 3% FBS in PBS for 30 minutes at room temperature. Samples were then incubated with primary antibody at 1/50 for 1 hour.

    See Abreview

文献

This product has been referenced in:
  • Polpitiya Arachchige S  et al. Analysis of Select Herpes Simplex Virus 1 (HSV-1) Proteins for Restriction of Human Immunodeficiency Virus Type 1 (HIV-1): HSV-1 gM Protein Potently Restricts HIV-1 by Preventing Intracellular Transport and Processing of Env gp160. J Virol 92:N/A (2018). Read more (PubMed: 29093081) »
  • Decker CE  et al. Tmem178 acts in a novel negative feedback loop targeting NFATc1 to regulate bone mass. Proc Natl Acad Sci U S A 112:15654-9 (2015). Read more (PubMed: 26644563) »
See all 2 Publications for this product

客户评价及客户问答

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (cos7)
Specification
cos7
Permeabilization
No
Fixative
Formaldehyde

Ms. Ya Zhou

Verified customer

提交于 Dec 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Breast cancer cells (HA tagged expression plasmid)
Specification
Breast cancer cells (HA tagged expression plasmid
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton in PBS
Blocking step
1% BSA and 3% FBS in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Dr. Manikandan Periyasamy

Verified customer

提交于 Sep 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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