重组Anti-H Cadherin抗体[EPR9621] (ab167407)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9621] to H Cadherin
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-H Cadherin抗体[EPR9621]
参阅全部 H Cadherin 一抗 -
描述
兔单克隆抗体[EPR9621] to H Cadherin -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human H Cadherin aa 100-200. The exact sequence is proprietary.
Database link: P55290 -
阳性对照
- Fetal heart lysate; Human heart and Human brain tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9621 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab167407于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 78 kDa.
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IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 78 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth. -
组织特异性
Highly expressed in heart. In the CNS, expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. No expression detected in cerebellum or spinal cord. -
序列相似性
Contains 5 cadherin domains. -
发展阶段
Expressed at higher levels in adult brain than in developing brain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1012 Human
- Entrez Gene: 12554 Mouse
- Entrez Gene: 192248 Rat
- Omim: 601364 Human
- SwissProt: P55290 Human
- SwissProt: Q9WTR5 Mouse
- Unigene: 654386 Human
- Unigene: 334841 Mouse
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别名
- CAD13_HUMAN antibody
- Cadherin-13 antibody
- CDH13 antibody
see all
图片
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ab167407 showing -ve staining in Human normal liver.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab167407 showing -ve staining in Human normal colon.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab167407 showing -ve staining in Human normal pancreas.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab167407 showing +ve staining in Human normal kidney.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human brain tissue, labeling H Cadherin with ab167407 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling H Cadherin with ab167407 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-H Cadherin antibody [EPR9621] (ab167407) at 1/2195 dilution + Human Heart at 20 µg
Secondary
Goat anti-rabbit IgG (HRP) with minimal cross reactivity with human IgG at 1/2000 dilution
Predicted band size: 78 kDa
Exposure time: 5 secondsDouble bands observed should be isoforms
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab167407 被引用在 3 文献中.
- Rorabaugh BR et al. Myocardial fibrosis, inflammation, and altered cardiac gene expression profiles in rats exposed to a predator-based model of posttraumatic stress disorder. Stress 23:125-135 (2020). PubMed: 31347429
- Dyikanov DT et al. Optimization of CRISPR/Cas9 Technology to Knock Out Genes of Interest in Aneuploid Cell Lines. Tissue Eng Part C Methods 25:168-175 (2019). PubMed: 30747044
- Camolotto SA et al. FoxA1 and FoxA2 drive gastric differentiation and suppress squamous identity in NKX2-1-negative lung cancer. Elife 7:N/A (2018). PubMed: 30475207