Anti-Guanylyl Cyclase alpha 1/GUCY1A3抗体(ab85445)
Key features and details
- Rabbit polyclonal to Guanylyl Cyclase alpha 1/GUCY1A3
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Guanylyl Cyclase alpha 1/GUCY1A3抗体
参阅全部 Guanylyl Cyclase alpha 1/GUCY1A3 一抗 -
描述
兔多克隆抗体to Guanylyl Cyclase alpha 1/GUCY1A3 -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody gave a positive signal in the following tissue lysates: Rat kidney; Rat lung; Mouse lung; Rat heart. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85445于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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IHC-P |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
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说明 |
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ICC/IF
Use a concentration of 5 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). |
靶标
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序列相似性
Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 1 guanylate cyclase domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 2982 Human
- Entrez Gene: 60596 Mouse
- Entrez Gene: 497757 Rat
- Omim: 139396 Human
- SwissProt: Q02108 Human
- SwissProt: Q9ERL9 Mouse
- SwissProt: P19686 Rat
- Unigene: 24258 Human
see all -
别名
- GC S alpha 1 antibody
- GC SA3 antibody
- GCS alpha 1 antibody
see all
图片
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ab85445 staining Guanylyl Cyclase alpha 1/GUCY1A3 in MCF7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab85445 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody (ab85445) at 1 µg/ml
Lane 1 : Kidney (Rat) Tissue Lysate
Lane 2 : Lung (Rat) Tissue Lysate
Lane 3 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Additional bands at: 48 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
IHC image of Guanylyl Cyclase alpha 1/GUCY1A3 staining in human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85445, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab85445 尚未被引用在任何文献中。