Anti-GSTM4抗体(ab233281)
Key features and details
- Rabbit polyclonal to GSTM4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-GSTM4抗体 -
描述
兔多克隆抗体to GSTM4 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cynomolgus monkey -
免疫原
Recombinant fragment (His-T7-tag) corresponding to Human GSTM4 aa 1-250. Expressed in E.coli.
Database link: Q03013 -
阳性对照
- WB: Recombinant human GSTM4 protein; Human liver and lung lysates; Mouse liver and lung lysates. IHC-P: Human breast cancer, glioma, prostate, colorectal cancer, liver and stomach tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab233281于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 26 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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说明 |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 26 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
靶标
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功能
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Active on 1-chloro-2,4-dinitrobenzene. -
组织特异性
Expressed in a wide variety of tissues. -
序列相似性
Belongs to the GST superfamily. Mu family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 2948 Human
- Entrez Gene: 14865 Mouse
- GenBank: NM_000850.3 Human
- Omim: 138333 Human
- SwissProt: Q9TSM5 Cynomolgus monkey
- SwissProt: Q03013 Human
- SwissProt: P19639 Mouse
- Unigene: 348387 Human
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别名
- Glutathione S transferase M4 antibody
- Glutathione S transferase Mu 4 antibody
- Glutathione S-transferase Mu 4 antibody
see all
图片
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Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for GSTM4 using ab233281 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Anti-GSTM4 antibody (ab233281) at 2 µg/ml + Human liver lysate
Secondary
HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/ml
Predicted band size: 26 kDa -
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for GSTM4 using ab233281 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human glioma tissue stained for GSTM4 using ab233281 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human prostate tissue stained for GSTM4 using ab233281 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining..
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Formalin-fixed, paraffin-embedded human liver tissue stained for GSTM4 using ab233281 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for GSTM4 using ab233281 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Anti-GSTM4 antibody (ab233281) at 2 µg/ml + Human lung lysate
Secondary
HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/ml
Predicted band size: 26 kDa -
Anti-GSTM4 antibody (ab233281) at 2 µg/ml + Mouse liver lysate
Secondary
HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/ml
Predicted band size: 26 kDa -
Anti-GSTM4 antibody (ab233281) at 2 µg + Mouse lung lysate
Secondary
HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/ml
Predicted band size: 26 kDa -
Anti-GSTM4 antibody (ab233281) at 2 µg + Recombinant human GSTM4 protein
Predicted band size: 26 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab233281 被引用在 1 文献中.
- Xu M et al. Proteomic Reveals Reasons for Acquired Drug Resistance in Lung Cancer Derived Brain Metastasis Based on a Newly Established Multi-Organ Microfluidic Chip Model. Front Bioeng Biotechnol 8:612091 (2020). PubMed: 33415100