Application
ELISA
Sample
Aedes aegypti (Mosquito) Recombinant protein (GST-tagged mosquito protein made in e coli)
Specification
GST-tagged mosquito protein made in e coli
Type
Direct
Other product details
Incubation time
30 minute(s) · Temperature: 25°C · Diluent: 1%BSA in PBS
Dilution
1/5000
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Graph of ELISA measuring levels of bound GST-tagged mosquito proteins. 5 ?g of target protein was coated onto an ELISA plate and incubated overnight at 4¯C. The plate was washed and GST-tagged mosquito proteins were incubated for an hour at RT. The proteins were washed off, GST-HRP was diluted 1/5000 in 1%BSA in PBS and added for 30 min at RT. The plate was washed and TMB substrate was added for 20 min at RT. Stop solution was added and the O.D. of the wells read at 450 nm.
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提交于 Dec 08 2014