概述

  • 产品名称

  • 描述

    兔多克隆抗体to GST
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC/IF, ELISA, WB, IP, ChIPmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Glutathione-S-Transferase (GST) from Schistosoma japonicum.

性能

应用

Our Abpromise guarantee covers the use of ab19256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/400.
ELISA 1/100 - 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IP Use a concentration of 1 - 4 µg/ml.
ChIP Use 4 µg for µg of chromatin.

靶标

  • 相关性

    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • 别名

    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

图片

  • All lanes : Anti-GST antibody (ab19256) at 1/15000 dilution

    Lane 1 : HEK293 lysate at 0.1 µg
    Lane 2 : HEK293 lysate at 0.3 µg
    Lane 3 : HEK293 lysate at 1 µg

    Predicted band size: 24 kDa


    Exposure time: 3 seconds


    Detected by Chemiluminescence. 

     

  • All lanes : Anti-GST antibody (ab19256) at 1/5000 dilution

    Lane 1 : GST tagged purified protein from E. Coli BL21 cells at 0.1 µg
    Lane 2 : GST tagged purified protein from E. Coli BL21 cells at 0.2 µg
    Lane 3 : GST tagged purified protein from E. Coli BL21 cells at 0.4 µg

    Secondary
    All lanes : A HRP conjugated donkey polyclonal to rabbit at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 72 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds

    See Abreview

  • NogoΔ20-induced Rho activation depends on internalization. (A–C) Rho activation levels were examined in PC12 cells that were either untreated (control; A), treated with 300 nM NogoΔ20 for 30 min at 37°C in absence of mutant PincherG68E (B), or treated with 300 nm NogoΔ20 treated for 30 min at 37°C in the presence of mutant PincherG68E (C). Active GTP-bound Rho was detected by incubation with GST-tagged Rhotekin-RBD and immunostaining using ab19256. Bar, 20 µm. (D) Densitometric quantification of staining from three independent experiments. Data are normalized to the mean of the untreated group ± SEM (error bars); asterisks marks highly significant differences between untreated, NogoΔ20-treated, or NogoΔ20- and dn PincherG68E-treated cells (three experiments; 30–50 cells per experiment; ***, P < 0.001; Student’s t test). (E) PC12 cells were either left untreated or transfected with dn PincherG68E construct. All cells were then incubated with 300 nM NogoΔ20 for 30 min at 37°C. Extracted

文献

This product has been referenced in:

  • Deng L  et al. Ubiquitination of Rheb governs growth factor-induced mTORC1 activation. Cell Res 29:136-150 (2019). Read more (PubMed: 30514904) »
  • Shang Z  et al. LncRNA PCAT1 activates AKT and NF-?B signaling in castration-resistant prostate cancer by regulating the PHLPP/FKBP51/IKKa complex. Nucleic Acids Res N/A:N/A (2019). Read more (PubMed: 30773595) »
See all 43 Publications for this product

客户评价及客户问答

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1-6 of 6 Abreviews

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cells)
Total protein in input
400 µg
Immuno-precipitation step
Other - Dynabeads M-270 Epoxy using Dynabeads Antibody coupling kit (Life Science Technologies)
Specification
HeLa cells

Dr. Armen Petrosyan

Verified customer

提交于 Nov 19 2018

Abreviews
Application
Western blot
Loading amount
1 µg
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Sample
Mouse Recombinant protein (made in E.coli)
Specification
made in E.coli
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Nov 18 2014

Abreviews
Application
Western blot
Sample
Escherichia coli Purified protein (Recombinant GST protein purified from E. coli)
Loading amount
5 µg
Specification
Recombinant GST protein purified from E. coli
Gel Running Conditions
Reduced Denaturing (10% Tris-Bicine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Jul 30 2010

Application
Western blot
Sample
Mouse Recombinant protein (Western blot to analyse GST pulldown)
Loading amount
15 µg
Specification
Western blot to analyse GST pulldown
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

提交于 May 14 2010

Abreviews
Application
ChIP
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (Whole cell)
Specification
Whole cell
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: High quality formaldehyde
Detection step
Semiquantitative PCR

Azharul Islam

Verified customer

提交于 Feb 02 2009

Abreviews
Application
Western blot
Sample
Escherichia coli Purified protein (GST-tagged protein expressed in BL21* cells, fract)
Loading amount
0.1 µg
Specification
GST-tagged protein expressed in BL21* cells, fract
Gel Running Conditions
Reduced Denaturing (8% SDS page gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Hanneke Okkenhaug

Verified customer

提交于 Nov 05 2008

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