Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Loading amount
40 µg
Specification
HEK293T
Treatment
X gene transfection for 24hrs
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% Milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 51 for Y279 47 for Y kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Chien Hsin Lee
Verified customer
提交于 Sep 09 2009