Application
Western blot
Sample
Human Cell lysate - whole cell (Macrophages)
Loading amount
40 µg
Specification
Macrophages
Treatment
Thapsigargin for 24 hours
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 2.5% milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 94 kDa Non-specific: 75 kDa
Positive control
Cells treated with Thapsigargin
Additional data
Additional Notes
GRP94 expression increased following the treatment with thapsigargin (TG). There is a prominent band around 75 kDa (it could be GRP78/BiP), other than that the antibody works fine.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 30 2011