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Tags & Cell Markers Subcellular Markers Organelles ER

Anti-GRP94抗体(ab3674)

  • Datasheet
Reviews (10) Submit a question References (19)

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Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab3674)
  • Western blot - Anti-GRP94 antibody (ab3674)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab3674)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody (ab3674)
  • Western blot - Anti-GRP94 antibody (ab3674)

Key features and details

  • Rabbit polyclonal to GRP94
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-GRP94抗体
    参阅全部 GRP94 一抗
  • 描述

    兔多克隆抗体to GRP94
  • 宿主

    Rabbit
  • 特异性

    At a very high concentration (<1/100), this antibody may recognise PDI and calreticulin. We recommend using this antibody at a relatively high dilution.
  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Whole protein from dog pancreas.

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
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    • ER Proteins
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相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human GRP94 protein (ab87144)

应用

Our Abpromise guarantee covers the use of ab3674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/5000. Detects a band of approximately 94 kDa. Can be used at dilutions up to 1/30,000.
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. Used at a concentration of 1/100 for 30 min on human renal carcinoma cell (see Abreview).

靶标

  • 功能

    Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
  • 序列相似性

    Belongs to the heat shock protein 90 family.
  • 细胞定位

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P14625 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7184 Human
    • Entrez Gene: 22027 Mouse
    • Omim: 191175 Human
    • SwissProt: P14625 Human
    • SwissProt: P08113 Mouse
    • Unigene: 192374 Human
    • Unigene: 87773 Mouse
    • 别名

      • 94 kDa glucose regulated protein antibody
      • 94 kDa glucose-regulated protein antibody
      • ECGP antibody
      • Endoplasmin antibody
      • Endothelial cell (HBMEC) glycoprotein antibody
      • ENPL_HUMAN antibody
      • Glucose regulated protein 94kDa antibody
      • gp96 antibody
      • gp96 homolog antibody
      • GRP 94 antibody
      • GRP-94 antibody
      • Heat shock protein 90 kDa beta member 1 antibody
      • heat shock protein 90kDa beta (Grp94), member 1 antibody
      • Heat shock protein, 90 kDa, beta, 1 antibody
      • HSP90B1 antibody
      • Stress inducible tumor rejection antigen GP96 antibody
      • TRA1 antibody
      • tumor rejection antigen (gp96) 1 antibody
      • Tumor rejection antigen 1 antibody
      • Tumor rejection antigen gp96 antibody
      • Tumor rejection antigen-1 (gp96) antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab3674)
      Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab3674)
      Immunostaining of mouse embryonic fibroblasts with ab3674. (See Nakamura et al. 2001).
    • Western blot - Anti-GRP94 antibody (ab3674)
      Western blot - Anti-GRP94 antibody (ab3674)
      Anti-GRP94 antibody (ab3674) at 1/2000 dilution + Cell lysates prepared from mouse fibroblasts


      Western blot of mouse fibroblasts using ab3764 at 1/2000.
    • Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab3674)
      Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab3674)This image is courtesy of an anonymous Abreview
      ab3674 at a 1/100 dilution staining human renal cell carcinoma cells by ICC/IF. The cells were permeabilized with 0.2% triton X100 and then blocked with 1%BSA, 4% goat serum prior to incubation with the primary 1antibody for 30 min at 37 °C. Bound antibody was detected using an Alexa fluor ® 488 Goat anti-Rabbit IgG (H+L).

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody (ab3674)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody (ab3674)

      Ab3674 (1/1000) staining GPR94 in human stomach using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of peptic and parietal cells of the gastric gland.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    • Western blot - Anti-GRP94 antibody (ab3674)
      Western blot - Anti-GRP94 antibody (ab3674)Image courtesy of an anonymous Abreview.
      All lanes : Anti-GRP94 antibody (ab3674) at 1/2000 dilution

      Lane 1 : Whole cell lysate prepared from murine 3T3 cells
      Lane 2 : Whole cell lysate prepared from murine 3T3 cells, treated with 2.5 ug/ml Tunicamycin 10 hours

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : HRP conjugated goat anti-rabbit IgG at 1/2000 dilution

      Developed using the ECL technique.

      Observed band size: 94 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds

      See Abreview

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (19)

    发表研究结果有使用 ab3674?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3674 被引用在 19 文献中.

    • Jiang L  et al. Inhibition of microRNA-103 attenuates inflammation and endoplasmic reticulum stress in atherosclerosis through disrupting the PTEN-mediated MAPK signaling. J Cell Physiol 235:380-393 (2020). PubMed: 31232476
    • Wolf-Dennen K  et al. Exosomal communication by metastatic osteosarcoma cells modulates alveolar macrophages to an M2 tumor-promoting phenotype and inhibits tumoricidal functions. Oncoimmunology 9:1747677 (2020). PubMed: 32313728
    • Shang A  et al. Exosomal miR-183-5p promotes angiogenesis in colorectal cancer by regulation of FOXO1. Aging (Albany NY) 12:8352-8371 (2020). PubMed: 32364530
    • Chiritoiu M  et al. EDEM1 Drives Misfolded Protein Degradation via ERAD and Exploits ER-Phagy as Back-Up Mechanism When ERAD Is Impaired. Int J Mol Sci 21:N/A (2020). PubMed: 32423001
    • Zeng Y  et al. Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesis. J Extracell Vesicles 8:1629865 (2019). PubMed: 31258881
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 10 Abreviews or Q&A

    Western blot abreview for Anti-GRP94 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (hepatocytes)
    Specification
    hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 25 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GRP94 antibody - ER Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Bonemarrow)
    Specification
    Bonemarrow
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 25 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GRP94 antibody - ER Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Fibroblast)
    Specification
    Fibroblast
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1 Triton X-100
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 23 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GRP94 antibody - ER Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (3T3)
    Specification
    3T3
    Fixative
    Formaldehyde
    Permeabilization
    Yes - Saponin
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 31 2011

    Western blot abreview for Anti-GRP94 antibody - ER Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Macrophages)
    Loading amount
    40 µg
    Specification
    Macrophages
    Treatment
    Thapsigargin for 24 hours
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 30 2011

    Western blot abreview for Anti-GRP94 antibody - ER Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (3T3)
    Loading amount
    50 µg
    Specification
    3T3
    Treatment
    2.5 ug/ml Tunicamycin 10 hours
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 28 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GRP94 antibody - ER Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293 EBNA)
    Specification
    HEK293 EBNA
    Fixative
    3% Paraformaldehyde
    Permeabilization
    No
    Blocking step
    NH4Cl as blocking agent for 15 minute(s) · Concentration: 0.27% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 22 2010

    Western blot abreview for Anti-GRP94 antibody - ER Marker

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (lymphnode and DCEK mouse fibroblast cell)
    Loading amount
    30 µg
    Specification
    lymphnode and DCEK mouse fibroblast cell
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 20 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GRP94 antibody - ER Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100 (10 mins)
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More

    Dr. Dan Tattersall

    Verified customer

    提交于 Nov 10 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GRP94 antibody - ER Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Renal Carcinoma Cell Line)
    Specification
    Renal Carcinoma Cell Line
    Fixative
    Paraformaldehyde
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 4%
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 12 2006

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