重组
RabMAb

Anti-GRP78 BiP抗体[EPR4040(2)] (ab108613)

概述

  • 产品名称
    Anti-GRP78 BiP抗体[EPR4040(2)]
    参阅全部 GRP78 BiP 一抗
  • 描述
    兔单克隆抗体[EPR4040(2)] to GRP78 BiP
  • 宿主
    Rabbit
  • 特异性
    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human GRP78 BiP aa 1-100. The exact sequence is proprietary.
    (Peptide available as ab176143)

  • 阳性对照
    • WB: HeLa, LnCaP, ECV-304, C6, NIH/3T3, PC-12, C6 (Rat glial tumor glial cell), RAW264.7 and HepG2 cell lysates. IHC-P: Human brain, ovarian cancer and testis tissue.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    EPR4040(2)
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab108613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 72 kDa).Can be blocked with GRP78 BiP peptide (ab176143).
IHC-P 1/250 - 1/500.

See IHC antigen retrieval protocols. (Heat to 98oC, allow to cool for 10-20 minutes) 

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

靶标

  • 功能
    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
  • 疾病相关
    Autoantigen in rheumatoid arthritis.
  • 序列相似性
    Belongs to the heat shock protein 70 family.
  • 细胞定位
    Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 78 kDa glucose regulated protein antibody
    • 78 kDa glucose-regulated protein antibody
    • AL022860 antibody
    • AU019543 antibody
    • BIP antibody
    • D2Wsu141e antibody
    • D2Wsu17e antibody
    • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
    • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
    • Epididymis secretory sperm binding protein Li 89n antibody
    • FLJ26106 antibody
    • Glucose Regulated Protein 78kDa antibody
    • GRP 78 antibody
    • GRP-78 antibody
    • GRP78 antibody
    • GRP78_HUMAN antibody
    • Heat shock 70 kDa protein 5 antibody
    • Heat Shock 70kDa Protein 5 antibody
    • Heat shock protein family A (Hsp70) member 5 antibody
    • HEL S 89n antibody
    • Hsce70 antibody
    • HSPA 5 antibody
    • HSPA5 antibody
    • Immunoglobulin Heavy Chain Binding Protein antibody
    • Immunoglobulin heavy chain-binding protein antibody
    • mBiP antibody
    • MIF2 antibody
    • Sez7 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian cancer tissue sections labeling GRP78 BiP with Purified ab108613 at 1:500 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylinwas used as a counterstain.
  • Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 0.1 µg/ml (purified) + C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 72 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue using unpurified ab108613 at 1/250

  • All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/5000 dilution (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10ug/ml
    tunicamycin for 12 hours whole cell lysates
    Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : MCF-7 (Human breast adenocarcinoma epithelial cell) treated with 10ug/ml
    tunicamycin for 48 hours whole cell lysates
    Lane 5 : MEF (Mouse embryonic fibroblast) whole cell lysates
    Lane 6 : MEF (Mouse embryonic fibroblast) treated with 10ug/ml tunicamycin for 48 hours whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 72 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

    Tunicamycin induces the expression of GRP78 BiP reported by PMID 22859938

    Exposure time:
    Lanes 1 and 2: 3 seconds 
    Lanes 3 and 4: 30 seconds
    Lanes 5 and 6: 5 seconds

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue using unpurified ab108613 at 1/250

  • All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution (Unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : LnCaP cell lysate
    Lane 3 : ECV-304 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 72 kDa
    Observed band size: 78 kDa (why is the actual band size different from the predicted?)

  • All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution (Unpurified)

    Lane 1 : C6 cell lysate
    Lane 2 : NIH/3T3 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : RAW264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 72 kDa
    Observed band size: 78 kDa (why is the actual band size different from the predicted?)

文献

This product has been referenced in:
  • Feng Y  et al. Selective Histone Deacetylase 6 Inhibitor 23BB Alleviated Rhabdomyolysis-Induced Acute Kidney Injury by Regulating Endoplasmic Reticulum Stress and Apoptosis. Front Pharmacol 9:274 (2018). Read more (PubMed: 29632491) »
  • Shen J  et al. GRP78 haploinsufficiency suppresses acinar-to-ductal metaplasia, signaling, and mutantKras-driven pancreatic tumorigenesis in mice. Proc Natl Acad Sci U S A 114:E4020-E4029 (2017). Read more (PubMed: 28461470) »

See all 11 Publications for this product

客户评价及客户问答

Thank you for contacting us. Below is the protocol used to validate ab108613 in IHC-P. Please let me know if you need any additional information or assistance.

1. Solutions and reagents
1.1. Xylene
1.2. Ethanol, anhydrous denatured...

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"We only saw a faint band at 50 kDa using mouse and rat heart, which looks non-specific on our blots. I have not found the identity of this band in the literature. The blocking reagent used was 5% milk. We used our standard WB protocol (...

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Thank you for your reply.
If you were able to detect a different ER protein using the same samples then it doesn't seem like it was a problem with the sample preparation.
I would like to offer you a free of charge replacement of ab108613 (alt...

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Thank you for taking time to send us your protocol. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring prod...

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Merci de votre appel. Je suis ravie que vous souhaitiez participer a notre offre et tester ab108613 en immunofluorescence sur des cellules (ICC/IF). Le code de réduction correspondant est xx. Ce code de réduction est utilisable sous 120 jours et p...

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Thank you for contacting us, please find the requested CoC attached. Please let me know if you need any other information!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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