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Anti-Grp75/MOT抗体[JG1] (ab2799)

  • Datasheet
Reviews (12)Q&A (4)References (34)

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Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody [JG1] (ab2799)
  • Flow Cytometry - Anti-Grp75/MOT antibody [JG1] (ab2799)
  • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)
  • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)
  • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)
  • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)
  • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody [JG1] (ab2799)
  • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)

Key features and details

  • Mouse monoclonal [JG1] to Grp75/MOT
  • Suitable for: ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG3

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概述

  • 产品名称

    Anti-Grp75/MOT抗体[JG1]
    参阅全部 Grp75/MOT 一抗
  • 描述

    小鼠单克隆抗体[JG1] to Grp75/MOT
  • 宿主

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    Human
    IHC-P
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide corresponding to Mouse Grp75/MOT aa 661-679.
    Sequence:

    GSGSSGTGEQKEDQKEEKQ


    Database link: P38647
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • Recombinant Human Grp75/MOT protein (ab79145) can be used as a positive control in WB. IHC-P: Human testis tissue. ICC: A549, HMVEC, NS-1, P19, HeLa and human fibroblasts cells.
  • 常规说明

    Previously labelled as Grp75.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: PBS, BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • Primary antibody说明

    The HSP 70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP 70 family members include: HSP 70, a 70 kDa protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP 72, a 72 kDa protein which is induced exclusively under stress conditions; HSC 70, or cognate protein, is a 72 kDa constitutively expressed protein which is involved in uncoating clathrin-coated vesicles; GRP 78, or BiP, is a glucose-regulated 78 kDa protein localized to the endoplasmic reticulum; and mitochondrial HSP 70 (mtHSP 70, GRP 75 or mortalin) a 75 kDa protein that is found within the mitochondria. mtHSP 70 is a mitochondrial resident protein that is involved in protein translocation into the mitochondria. Preproteins cross the mitochondrial membranes in an extended conformation. This requires unfolding of preproteins before entering translocation pores in the mitochondrial outer membrane. Preprotein unfolding is thought to be mediated by mtHSP 70 and other inner membrane proteins of the mitochondria.
  • 克隆

    单克隆
  • 克隆编号

    JG1
  • 同种型

    IgG3
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cell cycle
    • Cell division
    • Cancer
    • Tumor biomarkers
    • Other
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial Biogenesis
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG3, kappa monoclonal [MG3-35] - Isotype Control (ab18394)
  • Positive Controls

    • Recombinant Human Grp75/MOT protein (ab79145)
  • Recombinant Protein

    • Recombinant Human Grp75/MOT protein (ab115711)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC/IF
Mouse
Human
IHC-P
Human
应用 Ab评论 说明
ICC/IF (2)
1/50 - 1/200.

Staining of mtHSP 70 in DAP.3 cells results in a worm-like staining pattern, consistent with mitochondrial localization.

IHC-P (1)
Use a concentration of 1 µg/ml.
Flow Cyt
1/100.

ab91537 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

 

说明
ICC/IF
1/50 - 1/200.

Staining of mtHSP 70 in DAP.3 cells results in a worm-like staining pattern, consistent with mitochondrial localization.

IHC-P
Use a concentration of 1 µg/ml.
Flow Cyt
1/100.

ab91537 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

 

靶标

  • 功能

    Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
  • 序列相似性

    Belongs to the heat shock protein 70 family.
  • 翻译后修饰

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位

    Mitochondrion.
  • Target information above from: UniProt accession P38646 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3313 Human
    • Entrez Gene: 15526 Mouse
    • Omim: 600548 Human
    • SwissProt: P38646 Human
    • SwissProt: P38647 Mouse
    • Unigene: 184233 Human
    • Unigene: 209419 Mouse
    • 别名

      • 75 kDa glucose regulated protein antibody
      • 75 kDa glucose-regulated protein antibody
      • CSA antibody
      • Glucose Regulated Protein antibody
      • Grp 75 antibody
      • GRP-75 antibody
      • GRP75 antibody
      • GRP75_HUMAN antibody
      • Heat shock 70 kDa protein 9 antibody
      • Heat shock 70kD protein 9 antibody
      • heat shock 70kDa protein 9 antibody
      • Heat shock 70kDa protein 9B antibody
      • Heat shock protein 74 kDa A antibody
      • Heat shock protein A antibody
      • Heat shock protein cognate 74 antibody
      • Hsc74 antibody
      • Hsp74 antibody
      • Hsp74a antibody
      • HSPA9 antibody
      • Hspa9a antibody
      • HSPA9B antibody
      • MGC4500 antibody
      • mitochondrial antibody
      • Mortalin 2 antibody
      • Mortalin antibody
      • Mortalin perinuclear antibody
      • Mortalin2 antibody
      • MOT 2 antibody
      • MOT antibody
      • MOT2 antibody
      • Mthsp70 antibody
      • p66 mortalin antibody
      • P66 MOT antibody
      • PBP74 antibody
      • Peptide binding protein 74 antibody
      • Peptide-binding protein 74 antibody
      • Stress 70 protein mitochondrial antibody
      • Stress 70 protein mitochondrial precursor antibody
      • Stress-70 protein antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)
      Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)

      Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Grp75/MOT (green) with ab2799. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA for 15 minutes at room temperature. Cells were incubated with (left panel) or without (right panel) ab2799 (1:50) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody (1:400) for 30 minutes at room temperature. F-Actin (red) was stained with Dylight 554 phalloidin, and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody [JG1] (ab2799)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody [JG1] (ab2799)

      Immunohistochemistry was performed on normal biopsies of deparaffinized human testis tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a Mouse Monoclonal Antibody recognizing Grp75/MOT (ab2799) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Flow Cytometry - Anti-Grp75/MOT antibody [JG1] (ab2799)
      Flow Cytometry - Anti-Grp75/MOT antibody [JG1] (ab2799)
      Overlay histogram showing HepG2 cells stained with ab2799 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2799, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)
      Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)
      ICC/IF image of ab2799 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2799, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)
      Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)

      Immunocytochemistry/ Immunofluorescence of P19 (Mouse embryonal carcinoma cell line) cells using ab2799.

    • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)
      Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)

      Immunocytochemistry/ Immunofluorescence of NS-1 (Mouse myeloma cell line) cells using ab2799.

    • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)
      Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)

      Immunocytochemistry/ Immunofluorescence of HMVEC (Human microvascular endothelial cell line) cells using ab2799.

    • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)
      Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)

      Immunocytochemistry/ Immunofluorescence of A549 (Human lung carcinoma cell line) cells using ab2799.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody [JG1] (ab2799)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody [JG1] (ab2799)

      ab2799 (1µg/ml) staining GrP75/MOT in human frontal cerebral cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is strong mitochondrial staining.
      Insert depicts negative control (no primary antibody).
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    • Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)
      Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)

      Immunocytochemistry/Immunofluorescence analysis of human fibroblasts labelling Grp75/MOT with ab2799.

    实验方案

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (34)

    发表研究结果有使用 ab2799?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2799 被引用在 34 文献中.

    • Trigo D  et al. The regulation of mitochondrial dynamics in neurite outgrowth by retinoic acid receptor ß signaling. FASEB J 33:7225-7235 (2019). PubMed: 30857414
    • Milani M  et al. DRP-1 functions independently of mitochondrial structural perturbations to facilitate BH3 mimetic-mediated apoptosis. Cell Death Discov 5:117 (2019). PubMed: 31341643
    • Hou S  et al. PCDHGB7 Increases Chemosensitivity to Carboplatin by Inhibiting HSPA9 via Inducing Apoptosis in Breast Cancer. Dis Markers 2019:6131548 (2019). PubMed: 31379979
    • Niemi NM  et al. Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis. Nat Commun 10:3197 (2019). PubMed: 31324765
    • Cheng W  et al. Elevated Mortalin correlates with poor outcome in hepatocellular carcinoma. Ann Diagn Pathol 42:59-63 (2019). PubMed: 31310900
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 16 Abreviews or Q&A

    Western blot abreview for Anti-Grp75/MOT antibody [JG1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human pulmonary endothelial cells)
    Gel Running Conditions
    Reduced Denaturing (10% SDS gel)
    Loading amount
    20 µg
    Specification
    Human pulmonary endothelial cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. PeiYi Su

    Verified customer

    提交于 Jul 20 2018

    Question

    Bonjour, Je fais parti de l'IFR 128, sur le site de lyon sud et je souhaiterais faire une commande chez Abcam. Je souhaiterais établir un devis avec la meilleure offre commerciale possible pour la commande de 2 tubes d'anticorps anti-Grp 75, ref : Ab2799. Pouvez vous me confirmer également qu'il est bien dirigé contre la protéine Hsp 70. Dans l'attente de votre retour, Cordialement,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 23 2011

    Answer

    Merci de nous avoir contactés. L'anticorps ab2799 est dirigé contre la protéine Grp75, référence SwissProt http://www.uniprot.org/uniprot/P38646 La liste des anti-Hsp70 (SwissProt http://www.uniprot.org/uniprot/P08107 ) est : https://www.abcam.com/index.html?pageconfig=searchresults&search=Hsp70&pt=1&sk=targ&sv=Hsp70&sn=Hsp70&l=1&fViewMore=1 J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Read More

    Abcam Scientific Support

    回复于 Sep 23 2011

    Western blot abreview for Anti-Grp75/MOT antibody [JG1]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (EL4)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    15 µg
    Specification
    EL4
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 06 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Grp75 / MOT antibody [JG1]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Trilogy
    Permeabilization
    Yes - tween20 (0.05%)
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    microwave euthanasia
    Read More

    Mr. Adrian Epstein

    Verified customer

    提交于 Aug 02 2011

    Western blot abreview for Anti-Grp75/MOT antibody [JG1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (HELA)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    HELA
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 08 2010

    Western blot abreview for Anti-Grp75 / MOT antibody [JG1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (MEF)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    MEF
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 08 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Grp75 / MOT antibody [JG1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cultured Cells (HeLa)
    Permeabilization
    Yes - 0.5% Triton-X
    Specification
    HeLa
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 12 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Grp75/MOT antibody [JG1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cultured Cells (Embryonic Fibroblasts)
    Permeabilization
    Yes - 0.5% Triton-x
    Specification
    Embryonic Fibroblasts
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 05 2010

    Western blot abreview for Anti-Grp75/MOT antibody [JG1]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 27 2009

    Western blot abreview for Anti-Grp75/MOT antibody [JG1]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Brain cortex)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    Brain cortex
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 07 2009

    1-10 of 16 Abreviews or Q&A

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