Anti-GRK1抗体[G8] (ab2775)

Submit a review Q&A (1)References (2)

Key features and details

  • Mouse monoclonal [G8] to GRK1
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Rat, Chicken, Cow, Dog, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-GRK1抗体[G8]
    参阅全部 GRK1 一抗

  • 描述

    小鼠单克隆抗体[G8] to GRK1

  • 宿主

    Mouse

  • 特异性

    Detects G-protein-associated rhodopsin kinase 1a (GRK1a).

  • 经测试应用

    适用于: ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat, Chicken, Cow, Dog, Human

  • 免疫原

    Full length native protein (purified) corresponding to Human GRK1.

  • 表位

    This antibody recognizes an epitope in the C-terminal region of GRK1a.

性能

应用

Our Abpromise guarantee covers the use of ab2775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/200.

靶标

  • 功能

    Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.

  • 组织特异性

    Retinal-specific. Expressed in rods and cones cells.

  • 疾病相关

    Night blindness, congenital stationary, Oguchi type 2

  • 序列相似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.

  • 翻译后修饰

    Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
    Farnesylation is required for full activity.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • EC=2.7.11.14 antibody
    • G protein-coupled receptor kinase 1 antibody
    • GPRK1 antibody
    • Grk1 antibody
    • Rhodopsin kinase antibody
    • RHOK antibody
    • RK antibody
    • RK_HUMAN antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:100) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunolocalization of GRK1 in bovine retina using ab2775.

文献 (2)

发表研究结果有使用 ab2775？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab2775 被引用在 2 文献中.

  • Wong BH  et al. Mfsd2a Is a Transporter for the Essential ?-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development. J Biol Chem 291:10501-14 (2016). PubMed: 27008858
  • Aguilà M  et al. Hsp90 inhibition protects against inherited retinal degeneration. Hum Mol Genet 23:2164-75 (2014). PubMed: 24301679

客户评价及客户问答

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Question

Customer would like to know if this antibody has specifically been tested with total eye extracts from rat or mouse.

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Answer

This antibody has not specifically been tested with total eye extracts. However, it has been proven to work in both rat and mouse cells in the the following applications: Western blot, Immunoprecipitation, Immunofluorescence. If you have any additional questions, please contact us again.

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