Anti-GRK1抗体[G8] (ab2775)

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Key features and details

  • Mouse monoclonal [G8] to GRK1
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Rat, Chicken, Cow, Dog, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-GRK1抗体[G8]
    参阅全部 GRK1 一抗

  • 描述

    小鼠单克隆抗体[G8] to GRK1

  • 宿主

    Mouse

  • 特异性

    Detects G-protein-associated rhodopsin kinase 1a (GRK1a).

  • 经测试应用

    适用于: ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat, Chicken, Cow, Dog, Human

  • 免疫原

    Full length native protein (purified) corresponding to Human GRK1.

  • 表位

    This antibody recognizes an epitope in the C-terminal region of GRK1a.

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab2775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/200.

靶标

  • 功能

    Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.

  • 组织特异性

    Retinal-specific. Expressed in rods and cones cells.

  • 疾病相关

    Night blindness, congenital stationary, Oguchi type 2

  • 序列相似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.

  • 翻译后修饰

    Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
    Farnesylation is required for full activity.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • EC=2.7.11.14 antibody
    • G protein-coupled receptor kinase 1 antibody
    • GPRK1 antibody
    • Grk1 antibody
    • Rhodopsin kinase antibody
    • RHOK antibody
    • RK antibody
    • RK_HUMAN antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:100) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunolocalization of GRK1 in bovine retina using ab2775.

文献 (2)

发表研究结果有使用 ab2775？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab2775 被引用在 2 文献中.

  • Wong BH  et al. Mfsd2a Is a Transporter for the Essential ?-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development. J Biol Chem 291:10501-14 (2016). PubMed: 27008858
  • Aguilà M  et al. Hsp90 inhibition protects against inherited retinal degeneration. Hum Mol Genet 23:2164-75 (2014). PubMed: 24301679

客户评价及客户问答

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Question

Customer would like to know if this antibody has specifically been tested with total eye extracts from rat or mouse.

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Answer

This antibody has not specifically been tested with total eye extracts. However, it has been proven to work in both rat and mouse cells in the the following applications: Western blot, Immunoprecipitation, Immunofluorescence. If you have any additional questions, please contact us again.

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