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Neuroscience Neurotransmission Intracellular Signaling Kinases

Anti-GRK1抗体[G8] (ab2775)

  • Datasheet
Submit a review Q&A (1)References (2)

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Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

Key features and details

  • Mouse monoclonal [G8] to GRK1
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Cow, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-GRK1抗体[G8]
    参阅全部 GRK1 一抗
  • 描述

    小鼠单克隆抗体[G8] to GRK1
  • 宿主

    Mouse
  • 特异性

    Detects G-protein-associated rhodopsin kinase 1a (GRK1a).
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Cow
    Human
    See all applications and species data
  • 免疫原

    Full length native protein (purified) corresponding to Human GRK1.

  • 表位

    This antibody recognizes an epitope in the C-terminal region of GRK1a.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • 纯度

    Protein G purified
  • Primary antibody说明

    The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events.
  • 克隆

    单克隆
  • 克隆编号

    G8
  • 同种型

    IgG1
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Heterotrimeric G Proteins
    • GPCR Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Neuroscience
    • Sensory System
    • Visual system

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human GRK1 protein (ab105126)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
ICC/IF
Mouse
Cow
Human
应用 Ab评论 说明
ICC/IF
1/100 - 1/200.
说明
ICC/IF
1/100 - 1/200.

靶标

  • 功能

    Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.
  • 组织特异性

    Retinal-specific. Expressed in rods and cones cells.
  • 疾病相关

    Night blindness, congenital stationary, Oguchi type 2
  • 序列相似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • 翻译后修饰

    Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
    Farnesylation is required for full activity.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession Q15835 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 131890 Human
    • Entrez Gene: 6011 Human
    • Entrez Gene: 24013 Mouse
    • Omim: 180381 Human
    • SwissProt: Q15835 Human
    • SwissProt: Q8WTQ7 Human
    • SwissProt: Q9WVL4 Mouse
    • Unigene: 103501 Human
    • Unigene: 680654 Human
    • Unigene: 721727 Human
    • Unigene: 257501 Mouse
    see all
  • 别名

    • EC=2.7.11.14 antibody
    • G protein-coupled receptor kinase 1 antibody
    • GPRK1 antibody
    • Grk1 antibody
    • Rhodopsin kinase antibody
    • RHOK antibody
    • RK antibody
    • RK_HUMAN antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:100) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunolocalization of GRK1 in bovine retina using ab2775.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (2)

    发表研究结果有使用 ab2775?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2775 被引用在 2 文献中.

    • Wong BH  et al. Mfsd2a Is a Transporter for the Essential ?-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development. J Biol Chem 291:10501-14 (2016). PubMed: 27008858
    • Aguilà M  et al. Hsp90 inhibition protects against inherited retinal degeneration. Hum Mol Genet 23:2164-75 (2014). PubMed: 24301679

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Question

    Customer would like to know if this antibody has specifically been tested with total eye extracts from rat or mouse.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 17 2004

    Answer

    This antibody has not specifically been tested with total eye extracts. However, it has been proven to work in both rat and mouse cells in the the following applications: Western blot, Immunoprecipitation, Immunofluorescence. If you have any additional questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Dec 17 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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