Key features and details
- Rabbit polyclonal to Granzyme B
- Suitable for: IHC-P, IHC-FoFr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
参阅全部 Granzyme B 一抗
描述兔多克隆抗体to Granzyme B
The immunogen used for this product shares 94% homology with Granzyme H. Cross-reactivity with this protein has not been confirmed experimentally.
经测试应用适用于: IHC-P, IHC-FoFrmore details
种属反应性与反应: Mouse, Rat, Human
预测可用于: Pig, Common marmoset
Synthetic peptide within Human Granzyme B aa 1-100. The exact sequence is proprietary.
Database link: P10144
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab4059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-FoFr||Use at an assay dependent concentration.|
功能This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
序列相似性Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain.
细胞定位Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
- Information by UniProt
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
Cytotoxic effector cells and regulatory T cells in human glioblastoma xenograft growing in immunocompetent rats.
Granzyme B-positive cytotoxic cells (ab4059 at a 1/75 dilution) in a GBM xenograft with rejection. Asterisks mark tissue lyzed by effector cells.
Immunohistochemical analysis of lung tissue from patients with idiopathic pulmonary fibrosis, staining Granzyme B with ab4059.
Tissue was fixed using paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% donkey serum in PBS containing 3% BSA for 45 minutes. Slides were incubated overnight at 4°C with primary antibody (1/100 in blocking solution). An AlexaFlour®568-conjugated goat anti-rabbit IgG was used as the secondary antibody.
ab4059 at 1/20 dilution staining rat spinal cord tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and blocked with serum prior to incubation with the antibody for 2 hours. A biotinylated goat anti-rabbit IgG was used as the secondary.
Human tonsil tissue stained with anti-Granzyme B antibody (ab4059).
ab4059 被引用在 79 文献中.
- Matsubara JA et al. Retinal Distribution and Extracellular Activity of Granzyme B: A Serine Protease That Degrades Retinal Pigment Epithelial Tight Junctions and Extracellular Matrix Proteins. Front Immunol 11:574 (2020). PubMed: 32318066
- Ireland L et al. Blockade of Stromal Gas6 Alters Cancer Cell Plasticity, Activates NK Cells, and Inhibits Pancreatic Cancer Metastasis. Front Immunol 11:297 (2020). PubMed: 32174917
- Qin G et al. NPM1 upregulates the transcription of PD-L1 and suppresses T cell activity in triple-negative breast cancer. Nat Commun 11:1669 (2020). PubMed: 32245950
- Kranjc Brezar S et al. Electrotransfer of siRNA to Silence Enhanced Green Fluorescent Protein in Tumor Mediated by a High Intensity Pulsed Electromagnetic Field. Vaccines (Basel) 8:N/A (2020). PubMed: 32012775
- Remic T et al. Development of Tumor Cell-Based Vaccine with IL-12 Gene Electrotransfer as Adjuvant. Vaccines (Basel) 8:N/A (2020). PubMed: 32121641