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Signal Transduction Signaling Pathway G Protein Signaling GPCR

Anti-GPR54抗体(ab100896)

  • Datasheet
  • SDS
Submit a review Submit a question References (4)

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Western blot - Anti-GPR54 antibody (ab100896)

    Key features and details

    • Rabbit polyclonal to GPR54
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-GPR54抗体
      参阅全部 GPR54 一抗
    • 描述

      兔多克隆抗体to GPR54
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat, Sheep, Goat, Cow, Moloney murine leukemia virus
    • 免疫原

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • 阳性对照

      • This antibody gave a positive signal in Human Spinal Cord tissue lysate.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • GPCR
      • Neuroscience
      • Neurology process
      • Neuroendocrinology
      • General neuroendocrinology

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab100896于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
    说明
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

    靶标

    • 功能

      Receptor for metastin (kisspeptin-54 or kp-54), a C-terminally amidated peptide of KiSS1. KiSS1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some breast carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/KISS1R system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins.
    • 组织特异性

      Most highly expressed in the pancreas, placenta and spinal cord, with lower-level of expression in peripheral blood leukocytes, kidney, lung, fetal liver, stomach, small intestine, testes, spleen, thymus, adrenal glands and lymph nodes. In the adult brain, expressed in the superior frontal gyrus, putamen, caudate nucleus, cingulate gyrus, nucleus accumbens, hippocampus, pons and amygdala, as well as the hypothalamus and pituitary. Expression levels are higher in early (7-9 weeks) than term placentas. Expression levels were increased in both early placentas and molar pregnancies and were reduced in choriocarcinoma cells. Expressed at higher levels in first trimester trophoblasts than at term of gestation. Also found in the extravillous trophoblast suggesting endocrine/paracrine activation mechanism.
    • 疾病相关

      Defects in KISS1R are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
      Defects in KISS1R are a cause of central precocious puberty (CEPREPU) [MIM:176400]. Precocious puberty is defined as the development of secondary sexual characteristics before the age of 8 years in girls and 9 years in boys. Central precocious puberty refers to a gonadotropin-dependent type which results from premature activation of the hypothalamic-pituitary-gonadal axis.
    • 序列相似性

      Belongs to the G-protein coupled receptor 1 family.
    • 细胞定位

      Cell membrane.
    • Target information above from: UniProt accession Q969F8 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 84634 Human
      • Entrez Gene: 114229 Mouse
      • Entrez Gene: 78976 Rat
      • Entrez Gene: 100144753 Sheep
      • Omim: 604161 Human
      • SwissProt: Q969F8 Human
      • SwissProt: Q91V45 Mouse
      • SwissProt: Q924U1 Rat
      • Unigene: 208229 Human
      • Unigene: 191035 Mouse
      • Unigene: 48681 Rat
      see all
    • 别名

      • AXOR 12 antibody
      • AXOR12 antibody
      • G protein coupled receptor 54 antibody
      • G-protein coupled receptor 54 antibody
      • G-protein coupled receptor OT7T175 antibody
      • GPCR 54 antibody
      • GPCR54 antibody
      • GPR 54 antibody
      • GPR54 antibody
      • hOT7T175 antibody
      • Hypogonadotropin 1 antibody
      • Hypogonadotropin-1 antibody
      • Hypogonadotropin1 antibody
      • KISS 1 receptor antibody
      • KISS 1R antibody
      • KiSS-1 receptor antibody
      • KiSS-1R antibody
      • KISS1 receptor antibody
      • Kiss1r antibody
      • Kisspeptins receptor antibody
      • KISSR_HUMAN antibody
      • Metastin receptor antibody
      • OT7T175 antibody
      see all

    图片

    • Western blot - Anti-GPR54 antibody (ab100896)
      Western blot - Anti-GPR54 antibody (ab100896)
      Anti-GPR54 antibody (ab100896) at 1 µg/ml + Human spinal cord tissue lysate - total protein (ab29188) at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 42 kDa
      Observed band size: 42 kDa


      Exposure time: 20 minutes


      GPR54 receptor contains a number of potential glycosylation sites (SwissProt) which may explain the migration pattern observed.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (4)

    发表研究结果有使用 ab100896?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab100896 被引用在 4 文献中.

    • Chang B  et al. Leptin and inflammatory factors play a synergistic role in the regulation of reproduction in male mice through hypothalamic kisspeptin-mediated energy balance. Reprod Biol Endocrinol 19:12 (2021). PubMed: 33472656
    • Wang S  et al. Effects of High-Glucose and High-Fat Condition on Estrogen Receptor- and Sexual Precocity-Related Genes in GT1-7 Cells. Med Sci Monit 26:e922860 (2020). PubMed: 32451371
    • Chen T  et al. p53 Mediates GnRH Secretion via Lin28/let-7 System in GT1-7 Cells. Diabetes Metab Syndr Obes 13:4681-4688 (2020). PubMed: 33299335
    • Cartwright JE & Williams PJ Altered placental expression of kisspeptin and its receptor in pre-eclampsia. J Endocrinol 214:79-85 (2012). WB ; Human . PubMed: 22526494

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