重组Anti-GPD2抗体[EPR14259] (ab188585)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14259] to GPD2
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GPD2抗体[EPR14259]
参阅全部 GPD2 一抗 -
描述
兔单克隆抗体[EPR14259] to GPD2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa cells; HeLa, MCF-7, NIH:OVCAR-3 and U87-MG cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14259 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab188585于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/310.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (3) |
1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 81 kDa).
|
IP |
1/30 - 1/50.
|
说明 |
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Flow Cyt (Intra)
1/310. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 81 kDa). |
IP
1/30 - 1/50. |
靶标
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通路
Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1. -
序列相似性
Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.
Contains 2 EF-hand domains. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 2820 Human
- Entrez Gene: 14571 Mouse
- Entrez Gene: 25062 Rat
- Omim: 138430 Human
- SwissProt: P43304 Human
- SwissProt: Q64521 Mouse
- SwissProt: P35571 Rat
- Unigene: 512382 Human
see all -
别名
- GDH2 antibody
- Glycerol 3 phosphate dehydrogenase 2 (mitochondrial) antibody
- Glycerol 3 phosphate dehydrogenase 2 antibody
see all
图片
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All lanes : Anti-GPD2 antibody [EPR14259] (ab188585) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : NIH:OVCAR-3 cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted? -
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling GPD2 with ab188585 at 1/310 dilution (red) compared to a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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Western blot aalysis of GPD2 in HeLa cell lysate immunoprecipitated using ab188585 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (9)
ab188585 被引用在 9 文献中.
- Ding Y et al. Effect of miR-143-3p from Extracellular Vesicles of Porcine Uterine Luminal Fluid on Porcine Trophoblast Cells. Animals (Basel) 12:N/A (2022). PubMed: 36496922
- Qu H et al. Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease. Diabetes 70:1372-1387 (2021). PubMed: 33741719
- Tan W et al. Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based Proteomics. Oxid Med Cell Longev 2020:8683404 (2020). PubMed: 32566106
- Chen J et al. The Potential of the FSP1cre-Pparb/d-/- Mouse Model for Studying Juvenile NAFLD. Int J Mol Sci 20:N/A (2019). PubMed: 31618976
- Thakur S et al. Metformin Targets Mitochondrial Glycerophosphate Dehydrogenase to Control Rate of Oxidative Phosphorylation and Growth of Thyroid Cancer In Vitro and In Vivo. Clin Cancer Res N/A:N/A (2018). PubMed: 29691295
- Liu X et al. Mitochondrial glycerol 3-phosphate dehydrogenase promotes skeletal muscle regeneration. EMBO Mol Med 10:N/A (2018). PubMed: 30389681
- Pinto SK et al. Expression of microRNAs and target proteins in skeletal muscle of rats selectively bred for high and low running capacity. Am J Physiol Endocrinol Metab 313:E335-E343 (2017). PubMed: 28465283
- Pehar M et al. Changes in Protein Expression and Lysine Acetylation Induced by Decreased Glutathione Levels in Astrocytes. Mol Cell Proteomics 15:493-505 (2016). PubMed: 26486419
- Xia M et al. Cell Type-Specific Effects of Mutant DISC1: A Proteomics Study. Mol Neuropsychiatry 2:28-36 (2016). PubMed: 27606318