Anti-GOLPH3/MIDAS抗体(ab98023)
Key features and details
- Rabbit polyclonal to GOLPH3/MIDAS
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-GOLPH3/MIDAS抗体
参阅全部 GOLPH3/MIDAS 一抗 -
描述
兔多克隆抗体to GOLPH3/MIDAS -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Zebrafish
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab98023于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
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| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
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ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. -
组织特异性
Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers. -
序列相似性
Belongs to the GOLPH3/VPS74 family. -
翻译后修饰
Phosphorylated. -
细胞定位
Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space. - Information by UniProt
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数据库链接
- Entrez Gene: 531359 Cow
- Entrez Gene: 64083 Human
- Entrez Gene: 66629 Mouse
- Entrez Gene: 78961 Rat
- Entrez Gene: 450085 Zebrafish
- Omim: 612207 Human
- SwissProt: Q9H4A6 Human
- SwissProt: Q9CRA5 Mouse
see all -
别名
- Coat protein antibody
- Coat protein GPP34 antibody
- FLJ90675 antibody
see all
图片
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Western blot - Anti-GOLPH3/MIDAS antibody (ab98023)All lanes : Anti-GOLPH3/MIDAS antibody (ab98023) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Lung (Human) Tissue Lysate
Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 4 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 5 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsThe predicted molecular weight of GOLPH3/MIDAS is 34-kDa. We are unsure why we are seeing an additional band in HeLa and A549 whole cell lysates. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Immunocytochemistry/ Immunofluorescence - Anti-GOLPH3/MIDAS antibody (ab98023)ICC/IF image of ab98023 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98023, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab98023 被引用在 11 文献中.
- Tan X et al. A protumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma. J Clin Invest 131:N/A (2021). PubMed: 32931483
- Zhu Z et al. Golgi phosphoprotein 3 promotes the proliferation of gallbladder carcinoma cells via regulation of the NLRP3 inflammasome. Oncol Rep 45:N/A (2021). PubMed: 33907835
- Arriagada C et al. GOLPH3 Regulates EGFR in T98G Glioblastoma Cells by Modulating Its Glycosylation and Ubiquitylation. Int J Mol Sci 21:N/A (2020). PubMed: 33238647
- Lu LQ et al. Regulation of the Golgi apparatus via GOLPH3-mediated new selective autophagy. Life Sci 253:117700 (2020). PubMed: 32335164
- Hong ZS et al. Tenacissoside H Induces Apoptosis and Inhibits Migration of Colon Cancer Cells by Downregulating Expression of GOLPH3 Gene. Evid Based Complement Alternat Med 2020:2824984 (2020). PubMed: 32454851