Application
Immunohistochemistry (Frozen sections)
Frozen OCT-embedded mouse spleen:
Section was air-dried
Section fixed in aceton, -20C, for 10 minutes
Peroxidase block Dako 10'
Protein block Dako 10'
Anti-mouse B220 (Biolegend, 0.5 mg/ml, 1:100) 30' RT
Goat anti-rat IgG PolymerHRP 20' RT
Colour developed with DAB
Counterstained with haematoxylin and mounted
Section was air-dried
Section fixed in aceton, -20C, for 10 minutes
Peroxidase block Dako 10'
Protein block Dako 10'
Anti-mouse B220 (Biolegend, 0.5 mg/ml, 1:100) 30' RT
Goat anti-rat IgG PolymerHRP 20' RT
Colour developed with DAB
Counterstained with haematoxylin and mounted
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Davide Lucchesi
Verified customer
提交于 Aug 10 2017