山羊抗大鼠IgG H&L (HRP) (ab97057)

概述

  • 产品名称
    山羊抗大鼠IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 宿主
    Goat
  • 靶标种属
    Rat
  • 特异性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG and with light chains common to other Rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • 经测试应用
    适用于: ICC, IHC-P, ELISA, WBmore details
  • 偶联物
    HRP

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 共轭说明
    Molar enzyme/ antibody protein ratio is 4:1
  • 克隆
    多克隆
  • 同种型
    IgG
  • 常规说明
    Part of the AbExcel range.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (as primary)
WB 1/2000 - 1/20000. Colorimetric: Use 1/5000-1/30000, Chemiluminescent: use 1/10000-1/50000

图片

  • IHC image of alpha tubulin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab64332, 5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97057, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml

    Lanes 1-6:  HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab97057) at 1/2000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab97057) at 1/10000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab97057) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 52 kDa

    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

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文献

This product has been referenced in:
  • Tabet R  et al. CUG initiation and frameshifting enable production of dipeptide repeat proteins from ALS/FTD C9ORF72 transcripts. Nat Commun 9:152 (2018). Read more (PubMed: 29323119) »
  • Zhu S  et al. Tanshinone-IIA attenuates the deleterious effects of oxidative stress in osteoporosis through the NF-?B signaling pathway. Mol Med Rep 17:6969-6976 (2018). Read more (PubMed: 29568934) »

See all 16 Publications for this product

客户评价及客户问答

Thank you for your reply. I am sorry that these antibodies did not perform as stated on the datasheet. It is regrettable these antibodies have not worked even though replacements have been tried.

As requested, I have asked our Finance depart...

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Thank you for your message.

I am sincerely sorry to hear this is still not working for you.

I am pleased to raise a credit note for the two items originally purchased. This can be used to pay towards the purchase of any other items ...

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Thank you for confirming these details and for your cooperation.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge alternative replaceme...

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Thank you for your reply.

Any anti rat IgG antibody tested in IHC-P should be suitable for your experiments and would be covered by the guarantee. I can suggest you may like to try the following. I can recommend to review the online datashee...

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Thank you for your message.

It is indeed disappointing that the results are not yet satisfactory, it is regrettably and I appreciate your concerns.

In this case, I would suggest it would be most beneficial to try a different secon...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your message and for providing this further information.

I am sorry there are no further suggestions to help improve the results on this occasion. Regrettably,one of the antibodies is probably not working.Unfortunately, it is n...

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Thank you for taking the time tocontact us and provide the details. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case fo...

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Thank you for your email and trying the protocol tips.

Please let me know if the the results do not improve. I will be happy to issue a credit note in case the antibody does not work as stated on the datasheet.

In case the results i...

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As requested, I have issued a free of charge replacement for ab7097, anti-rat IgG cross-absorbed to remove reactivity with mouse IgG. I apologize for the misunderstanding on our part and wish you the best of luck with your research.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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