山羊抗大鼠IgG H&L (HRP) (ab6845)

Submit a review Q&A (2)References (6)

Key features and details

  • Goat Anti-Rat IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr

概述

  • 产品名称

    山羊抗大鼠IgG H&L (HRP)
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Rat

  • 经测试应用

    适用于: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details

  • 免疫原

    Rat IgG whole molecule

  • 偶联物

    HRP

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 共轭说明

    Horseradish Peroxidase (HRP)

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6845于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/1500.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/1500.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (6)

发表研究结果有使用 ab6845？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6845 被引用在 6 文献中.

  • Funk N  et al. The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4. Sci Rep 9:4515 (2019). PubMed: 30872638
  • Ryu I  et al. eIF4A3 Phosphorylation by CDKs Affects NMD during the Cell Cycle. Cell Rep 26:2126-2139.e9 (2019). PubMed: 30784594
  • Vitali DG  et al. Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes. Elife 7:N/A (2018). PubMed: 29923829
  • Jores T  et al. Cytosolic Hsp70 and Hsp40 chaperones enable the biogenesis of mitochondrial ß-barrel proteins. J Cell Biol 217:3091-3108 (2018). PubMed: 29930205
  • Schneberger D  et al. Toll-like receptor 9 partially regulates lung inflammation induced following exposure to chicken barn air. J Occup Med Toxicol 11:31 (2016). IHC-P . PubMed: 27375768
  • Lesch HP  et al. Avidin fusion protein-expressing lentiviral vector for targeted drug delivery. Hum Gene Ther 20:871-82 (2009). IHC-FoFr . PubMed: 19419273

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Do you have another option of secondary antibody with those same characteristics but instead of HRP is biotynilated??

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Answer

Thank you for your reply.

The secondary ab98385 is a similar product: goat anti-rat IgG H&L conjugated to biotin and pre-adsorbed. This antibody shows minimal cross-reactivity to mouse and human IgGs. I hope this helps, please let me know if you need any additional information or assistance.

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Question

I need to by your secondary antibody (catalog # ab6845) Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP), but I need to know if it is pre-absorbed against mice.

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Answer

Thank you for contacting us.

The secondary antibody ab6845 has not been pre-adsorbed or tested for reactivity with mouse IgG. I would recommend the similar antibody ab98391, which has been pre-adsorbed and shows < 1% cross-reactivity to human and mouse IgGs. I hope this helps, please let me know if you need any additional information or assistance.

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