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Immunology Immunoglobulins Heavy Chain IgG

山羊抗大鼠IgG H&L (DyLight® 550) (ab96888)

  • Datasheet
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Goat Anti-Rat IgG H&L (DyLight® 550) (ab96888)

    Key features and details

    • Goat Anti-Rat IgG H&L (DyLight® 550)
    • Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: IHC-P, ICC/IF, Flow Cyt

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 6nm HRP HRP polymer PE PE Texas Red ® TRITC Unconjugated Unconjugated

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    概述

    • 产品名称

      山羊抗大鼠IgG H&L (DyLight® 550)
      参阅全部 IgG 二抗
    • 宿主

      Goat
    • 靶标种属

      Rat
    • 特异性

      By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG and with light chains common to other Rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
    • 经测试应用

      适用于: IHC-P, ICC/IF, Flow Cytmore details
    • 偶联物

      DyLight® 550. Ex: 562nm, Em: 576nm

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C.
    • 存储溶液

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 常规说明

      DyLight® 550 replaces DyLight® 549.
    • 研究领域

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Rat
      • IgG
      • Fluorophore
      • DyLight® 550

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab96888于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    1/50 - 1/500.
    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.
    说明
    IHC-P
    1/50 - 1/500.
    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.

    图片

    • Goat Anti-Rat IgG H&L (DyLight® 550) (ab96888)
      Goat Anti-Rat IgG H&L (DyLight® 550) (ab96888)
      Emission spectra of DyLight® fluorochromes available in our catalog.
      Line colors represent the approximate visible colors of the wavelength maxima.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (2)

    发表研究结果有使用 ab96888?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab96888 被引用在 2 文献中.

    • Xie C  et al. CCL7 contributes to angiotensin II-induced abdominal aortic aneurysm by promoting macrophage infiltration and pro-inflammatory phenotype. J Cell Mol Med 25:7280-7293 (2021). PubMed: 34189838
    • Chesarino NM  et al. E3 Ubiquitin Ligase NEDD4 Promotes Influenza Virus Infection by Decreasing Levels of the Antiviral Protein IFITM3. PLoS Pathog 11:e1005095 (2015). IHC-P . PubMed: 26263374

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    Thanks a lot for response. I am very sorry, I described our plan not correctly. We want to use two but not three ABs in our double staining. I try to repeat our question below with correction. We are planing to do double staining of Brdu and c-fos in freely floating slices of mice brains. For Brdu we will use: primary. anti-Brdu AB produced in rats, and secondary. goat anti-rat polyclonal AB IgG-H&L (DyLight® 550, ab96888). For c-fos we will use: primary. ither (!!!) rabbit polyclonal AB (ab7963) or (!!!) goat polyclonal AB (ab87655). I mean that we will need to find a secondary AB for a rabbit primary polyclonal AB (ab7963) and for a goat primary polyclonal AB (ab87655), which could be compatible with another secondary goat anti-rat polyclonal AB IgG-H&L (DyLight® 550, ab96888). Of course, each secondary ABs for c-fos staining will be used separately from each other but only in combination with goat anti-rat polyclonal AB IgG-H&L (DyLight® 550, ab96888). Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Jan 09 2012

    Answer

    Thank you for your reply. I hope that we may find the right product for you. We offer a large number of secondary antibodies against rabbit IgG. I see that you already have experience with our Dylight series of fluorescent secondaries. Since I do not know the excitation/emmision point tha you plan to use I cannot point to a specific product. However, my recommendations when choosing one are that you choose one raised in goat. In this way you need not be concerned about increased background staining as your other secondary against the rat primary is also raised in goat. Therefore using a block containing normal goat serum should work well for you. You may find these products using the search bar on the Abcam website. By typing Dylight and using the pulldown menu just to the right of the search field to select secondary antibodies.On the results page you will see a number of limitors to the left, These are labeled 'Target', 'Application', 'Host', etc. Click on the one labeled 'Target Species'. A short list will drop down. At the bottom of that list will be a link to 'View More'. If you click on that a pop-up window will appear that allows you to choose the species of interest, in this case rabbit. You may further limit that search by choosing theemmision/excitation pointthat is best for you under the 'Conjugation'limitorto the left. You may do much the same to find an appropriate anti-goat secondary. For this you will just need to change the target species from rabbit to goat. You should not be concerned with cross reactivity with the anti-Rat secondary in this way. Of note however is that we cannot offer a goat raised anti-goat secondary antibody. You will need to chose an antibody raised in another species. Rabbit and donkey are common choices. I would recommend that you add the serum of the host species that your secondary is raised in to your blocking solutions to try and limit the non-specific binding of that antibody to your tissue. i would still highly recommend using one of our EasyLink conjugation kits to directly conjugate a fluorecent tag to whichever antibody you choose. This way you can avoid high background caused by non-specific secondary binding, skip the secondary antibody incubation and wash stepsand conjugated antibody can be used straight away. I hope this information has been useful, please contact me if you have any questions. More information aboutEasyLink direct conjugation kits is available here: https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink

    Read More

    Abcam Scientific Support

    回复于 Jan 09 2012

    Question

    Hello, We are planing to do double staining of Brdu and c-fos in freely floating slices of mice brains. For Brdu we will use: primary. anti-Brdu AB produced in rats, and secondary. goat anti-rat polyclonal AB IgG-H&L (DyLight® 550, ab96888). For c-fos we will use: primary. rabbit polyclonal AB (ab7963) and goat polyclonal (ab87655), To choose a secondary AB for c-fos staining, and compatible with another secondary AB goat anti-rat AB IgG-H&L (DyLight® 550, ab96888), we need your recommendation. Please, would you inform us? Thanks, With best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jan 07 2012

    Answer

    Thank you for contacting Abcam. The difficulty with the experiment as you have set it up is that you would need three different secondary antibodies, each of which would need to not recognize the tissue or the other primary antibodies, and in reality you have only two targets. An easy and efficent way to remedy or circumvent any issue related to secondary antibodies is the directly congujate a tag, in your case a flouescent one, to your antibody. We offer a wide range of EasyLink direct fluorescent conjugation kits that would suit your needs. I've included a link below. https://www.abcam.com/index.html?pageconfig=resource&rid=13148#1 Let me know if there is anything else that I might do for you or if you have any questions.

    Read More

    Abcam Scientific Support

    回复于 Jan 07 2012

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