山羊抗大鼠IgG H&L (Alkaline Phosphatase) (ab6846)

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Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846)

    Key features and details

    • Goat Anti-Rat IgG H&L (Alkaline Phosphatase)
    • Conjugation: Alkaline Phosphatase
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr

    概述

    • 产品名称

      山羊抗大鼠IgG H&L (Alkaline Phosphatase)
      参阅全部 IgG 二抗

    • 宿主

      Goat

    • 靶标种属

      Rat

    • 经测试应用

      适用于: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details

    • 免疫原

      Rat IgG whole molecule

    • 偶联物

      Alkaline Phosphatase

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C.

    • 存储溶液

      Preservative: 0.1% Sodium azide
      Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.001% Zinc chloride, 50% Glycerol (glycerin, glycerine)
    • Concentration information loading...

    • 纯度

      Affinity purified

    • 纯化说明

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads.

    • 共轭说明

      Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

    • 克隆

      多克隆

    • 同种型

      IgG

    • 常规说明

      Part of the AbExcel range.

    • 研究领域

    相关产品

      应用

      The Abpromise guarantee

      Abpromise™承诺保证使用ab6846于以下的经测试应用

      “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

      应用 Ab评论 说明
      WB
      1/2000 - 1/20000.
      ELISA
      1/15000.
      Immunomicroscopy
      Use at an assay dependent concentration.
      Dot blot
      Use at an assay dependent concentration.
      ICC/IF
      1/200 - 1/1000.
      IHC-P
      Use at an assay dependent concentration.
      IHC-Fr
      Use at an assay dependent concentration.
      说明
      WB
      1/2000 - 1/20000.
      ELISA
      1/15000.
      Immunomicroscopy
      Use at an assay dependent concentration.
      Dot blot
      Use at an assay dependent concentration.
      ICC/IF
      1/200 - 1/1000.
      IHC-P
      Use at an assay dependent concentration.
      IHC-Fr
      Use at an assay dependent concentration.

      图片

      • Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846)
        Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846)
        All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml (Anti-Tubulin antibody [YL1/2] - Loading Control)

        All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        Lanes 1-2 : Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/5000 dilution (Goat polyclonal Secondary Antibody to Rat IgG)
        Lanes 3-4 : Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/20000 dilution (Goat polyclonal Secondary Antibody to Rat IgG)
        Lanes 5-6 : Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/50000 dilution (Goat polyclonal Secondary Antibody to Rat IgG)

        Developed using the ECL technique.

        Performed under reducing conditions.

      实验方案

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      文献 (2)

      发表研究结果有使用 ab6846？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab6846 被引用在 2 文献中.

      • Stromberg LR  et al. Presentation matters: Impact of association of amphiphilic LPS with serum carrier proteins on innate immune signaling. PLoS One 13:e0198531 (2018). PubMed: 29902192
      • Tarver MR  et al. Myosin Gene Expression and Protein Abundance in Different Castes of the Formosan Subterranean Termite (Coptotermes formosanus). Insects 3:1190-9 (2012). PubMed: 26466734

      客户评价及客户问答

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      Question

      Will this goat anti-rat secondary also detect mouse IgG?

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      Answer

      Thank you for your inquiry.

      I heard back from the supplying lab that this secondary will most likely cross-react with mouse IgG, due to similarity with ratIgG,although this hasn't been specifically tested.

      I know you said that this was a concern with some of your other mouse IgG primary antibodies with dual staining. I'd recommend using a pre-adsorbed antibody like donkey polyclonal Secondary Antibody to Rat IgG - H&L (AP), pre-adsorbed, ab102215, which will show minimal cross-reactivity with human and mouse.
      https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-AP-pre-adsorbed-ab102215.html
      Or ab102249, Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (AP), pre-adsorbed with minimal cross-reactivity with Cow, Horse, Human, Mouse, Rabbit, and Sheep.
      https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-AP-pre-adsorbed-ab102249.html
      I hope this information helps. Please contact us with any other questions.

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