Application
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry (PFA fixed)
Cells were fixed in 4% Paraformaldehyde.
Permeabilization: Triton X-100
Blocking: 5% BSA in PBS
Primary antibody: Rat anti-CD44 primary antibody
Secondary antibody ab150158 dilution 1/300
Counterstained with DAPI
Cells were fixed in 4% Paraformaldehyde.
Permeabilization: Triton X-100
Blocking: 5% BSA in PBS
Primary antibody: Rat anti-CD44 primary antibody
Secondary antibody ab150158 dilution 1/300
Counterstained with DAPI
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Egi Kardia
Verified customer
提交于 Mar 12 2021