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Immunology Immunoglobulins Heavy Chain IgG

山羊抗兔IgG H&L (TRITC) (ab6718)

  • Datasheet
  • SDS
Reviews (1)Q&A (8)References (41)

Product price, shipping and contact information

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Key features and details

  • Goat Anti-Rabbit IgG H&L (TRITC)
  • Conjugation: TRITC. Ex: 547nm, Em: 572nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

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概述

  • 产品名称

    山羊抗兔IgG H&L (TRITC)
    参阅全部 IgG 二抗
  • 宿主

    Goat
  • 靶标种属

    Rabbit
  • 经测试应用

    适用于: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • 免疫原

    Rabbit IgG, whole molecule

  • 偶联物

    TRITC. Ex: 547nm, Em: 572nm

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • 共轭说明

    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Fluorophore
    • Rhodamine

相关产品

    应用

    Our Abpromise guarantee covers the use of ab6718 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Immunomicroscopy Use at an assay dependent concentration.
    Flow Cyt Use at an assay dependent dilution.
    ICC/IF 1/1000 - 1/5000.
    IHC-P Use at an assay dependent concentration.
    IHC-Fr Use at an assay dependent concentration.
    ELISA 1/10000 - 1/50000.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (41)

    发表研究结果有使用 ab6718?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6718 被引用在 41 文献中.

    • Wang F  et al. miR-210 enhances mesenchymal stem cell-modulated neural precursor cell migration. Mol Med Rep 21:2405-2414 (2020). PubMed: 32323777
    • Yao D  et al. Discovery of novel ATAD2 bromodomain inhibitors that trigger apoptosis and autophagy in breast cells by structure-based virtual screening. J Enzyme Inhib Med Chem 35:713-725 (2020). PubMed: 32174193
    • Zhang X  et al. Ezrin Phosphorylation at T567 Modulates Cell Migration, Mechanical Properties, and Cytoskeletal Organization. Int J Mol Sci 21:N/A (2020). PubMed: 31936668
    • Liu H  et al. Heparin-poloxamer hydrogel-encapsulated rhFGF21 enhances wound healing in diabetic mice. FASEB J 33:9858-9870 (2019). PubMed: 31166803
    • Nam SY  et al. An osteoclastogenesis system, the RANKL/RANK signalling pathway, contributes to aggravated allergic inflammation. Br J Pharmacol N/A:N/A (2019). PubMed: 30737962
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-9 of 9 Abreviews or Q&A

    immunofluorescent staining of iNOS in mouse hepatocytes

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunomicroscopy
    Goat anti-Rabbit IgG H&L (TRITC) is good to label iNOS in mouse hepatocytes.

    Abcam user community

    Verified customer

    提交于 Aug 07 2015

    Question

    Thank you . Please add one same antibody from deifferent lot .
    The order no is
    1118866
    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Jan 07 2013

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1231536.
    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 Jan 07 2013

    Question

    Here is the reply from customer.

    I have tried with all the possible alterations even with the washing steps . We have been trying with alternatives for the last 6 months and the results still remains same.This made us suspect that the secondary antibody is faulty and is showing non specific flourochrome blotches.

    Thank You

    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Jan 03 2013

    Answer

    I am sorry hear about the disappointing results. If customer have purchased the secondary antibody from Abcam (ab6718) and if the problem is due to secondary antibody then I am happy to replace it for this customer.
    Please let me know the order number for secondary antibody.

    Read More

    Abcam Scientific Support

    回复于 Jan 03 2013

    Question

    Here is the reply from customer.
    Cell line used-HCT116 ;Human Colon Carcinoma cells
    There are similar patterns of red blotches even when there is no primary antibody used.The pattern remains the same when used with the required primary antibody but are non-specific in nature.
    The problem what we are suspecting is due to the non specific binding secondary antibody conjugated with TRITC i.e ab6718
    The antibody was stored at -20.
    I altered the permeabilization step,dilution and incubation time of both primary and secondary antibody
    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Dec 27 2012

    Answer

    Thank you for providing details.
    This is strange, if the red cells are seen even without primary antibody then the problem is either due to secondary antibody or due to protocol. I have checked the protocol and I find no problem with it other than the wash step which I assume is 3 washes of 5 minutes.
    You may need to try again, following the correct ICC/IF protocol steps.
    Please let me know if the results do not improve.

    Read More

    Abcam Scientific Support

    回复于 Dec 27 2012

    Question

    1) Abcam product code:
    ab6718
    2) Abcam order reference number or product lot number:1118866 July’2012
    3) Description of the problem (High Background, Low Signal, Non-specific staining etc):High Background and Non-specific staining
    4) Antibody storage conditions (temperature/reconstitution etc):-20
    5) Sample (Species/ Tissues/ Cell Type/ Cell Line etc):
    Species ( Hu, Ms, Rb,etc)Human
    TypeCell line
    6) Fixation Step: (Ethanol/ Methanol/ Accetone/ Paraformaldehyde/ Other/ Duration etc)
    Fixation: Yes/No
    yes
    Fixative agent and concentration
    Methanol:Acetone
    Fixation time 15min
    Fixation temperature - 20
    7) Antigen retrieval method: (Enzymatic Method/ Heat mediated technique etc)
    8) Permeabilization Step:Yes
    Permeabilization agent and concentrationPBS+0.25%triton X100
    Permeabilization time 15min
    Permeabilization temperature RT
    9) Blocking:yes
    Blocking agent and concentration
    1%BSA in PBST
    Blocking time 30min
    Blocking temperature RT
    Endogenous peroxidases blocked? NO
    Endogenous biotins blocked? NO
    10) Primary antibody (If more than one was used, describe in “additional notes”): ab110641
    Concentration or dilution 1:10000
    Diluent buffer 1%BSA in PBST
    Incubation time2hrs
    Incubation temperatureRT
    11) Secondary antibody:
    Species goat
    Reacts against
    Concentration or dilution 1:20000
    Diluent buffer 1%BSA in PBST
    Incubation time1hr
    Incubation temperature:
    Fluorochrome or enzyme conjugate
    TRITC
    12) Washing:

    Read More

    Abcam community

    Verified customer

    Asked on Dec 24 2012

    Answer

    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.
    I have read the details you have kindly provided and have following further questions for better understanding of the problem;
    - What was the cell line used? e.g. human liver cancer cell line.
    - Do you see difference in no primary control? Were the cells red in colour as seen with primary antibody?
    - Do you think the problem is due to ab6718 or ab110641.
    - What was the storage condition of antibody?
    - Did you change any step when doing repeat experiment?
    Thank you very much for your cooperation. I will look forward to hearing from you soon.

    Read More

    Abcam Scientific Support

    回复于 Dec 24 2012

    Question

    Hello

    Apologies for the delay in returning this email. I am still having difficulties in achieving optimal images with this antibody staining HUVECs. I am using 2 of your products: goat anti-rabbit IgG H&L (TRITC) secondary antibody (ab6718) and anti-CD31 antibody [EPR3094] (ab76533) and have had a huge amount of non-specific binding. I have tried various methods of optimisation including altering blocking solution/time/temperature, and concentrations of both antibody and yet am left with the same images. Please see those attached of a standard stain (titled ‘overnight’), and both a primary and secondary negative control.
    Any information would be greatly appreciated. This has taken a few months and still I am no further on in resolving this issue.
    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 18 2012

    Answer

    Thank you for your reply.

    I am very sorry to hear you are still experiencing problems with these products. According to the images sent it appears the secondary is still giving a lot of non specific background.

    If the antibody is still under the Abpromise guarantee I will be more than happy to send you a free of charge replacement for a different Goat anti-Rb secondary.

    Could you please provide your order number?

    The alternatives available are listed under the next link:

    https://www.abcam.com/index.html?pageconfig=searchresults&search=IgG&pt=7&sk=app&sv=3688&sn=ICC/IF&l=3&fViewMore=1

    I encourage you to have a look at it and decide which one fulfils your requirements.

    In regards to the primary anti-CD31, I am afraid this antibody has not been tested and validated in ICC, and therefore we cannot guarantee it will work in this application.

    I would suggest trying the new secondary I will send you free of charge, and in case you are still not obtaining the expected results, we will study more carefully the protocol and different possibilities.

    I then look forward for receiving your reply, and thank you for your cooperation. Have a great day.

    Read More

    Abcam Scientific Support

    回复于 Jul 18 2012

    Question

    Hello
    I recently used the following product (Goat anti-Rabbit IgG H&L (TRITC ) secondary antibody (ab6718)) to stain for CD31 on HUVECs. It seemed to be working correctly, but when I ran a negative control (without the primary (rabbit anti-human CD31)antibody) my staining was almost identical to when run with the primary (see attached file). Can you possibly suggest any reasons as to why this may have occurred? I ran at the same time as as a negative control (without secondary) which gave the expected results. The secondary was left on for 2 hours at a concentration of 1/200. Thank you.
    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 12 2012

    Answer

    Thank you for contacting us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I would appreciate if you could please send me the order details, so I can check there isn’t any particular issue with the lot number received and during the shipping process. It is also important for us in order to monitor our products’ quality.

    It will also be very helpful to have an image of the non primary incubation to compare it to the primary + secondary image sent.

    What is the isotype of the primary antibody? Has it been validated to work in ICC?

    In any case, all of our products are covered by our Abpromise guarantee; which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

    I look forward for receiving your reply.

    Read More

    Abcam Scientific Support

    回复于 Jun 12 2012

    Question

    I would like to ask you the difference between ab27089 and ab4055. I'd also like to know whether your goat anti-rabbit IgG (Rhodamine-conjugated); ab7087 or ab6718, is working well as a secondary antibody for these anti-CD8 polyclonal as well as the difference between ab7087 and ab6718. Thanks in advance.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 22 2006

    Answer

    Thank you for your enquiry. 1) Ab27089 is supplier as a pre diluted product and Ab4055 is not. 2) We do not have specific feedback in relation to certain secondary Abs functioning in conjunction with our antibodies; however a list of compatible secondary Abs are cited at the base of each online data sheet for you to choose from. I would expect any non rabbit species anti rabbit IgG with a suitable conjugation to function. 3)Looking at products Ab7087 and Ab6718 the only obvious difference that I am able to spot is the different concentrations of the product. I will pass this information onto my colleague sin business development since it is possible that we have added a duplicate product in error. Should you have any further questions then please do not hesitate to get back in contact with me.

    Read More

    Abcam Scientific Support

    回复于 Nov 22 2006

    Question

    Recently,i bought the ab6718 to use in a IF procedure. I need to know how to reconstitute this antibody (what buffer to use and in what volume).

    Read More

    Abcam community

    Verified customer

    Asked on Apr 20 2004

    Answer

    Thank you for your enquiry. According to the datasheet this antibody is in liquid form (the buffer is: 0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2) so you do not need to reconstitute it. The unit size is 1 mg and the concentration is 2 mg/ml. If you need anything further or any help then please let us know.

    Read More

    Abcam Scientific Support

    回复于 Apr 20 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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