山羊抗兔IgG H&L (PE)预吸附二抗(ab72465)
Key features and details
- Goat Anti-Rabbit IgG H&L (PE) preadsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ELISA
Related conjugates and formulations
概述
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产品名称
山羊抗兔IgG H&L (PE)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
经测试应用
适用于: Flow Cyt, ELISAmore details -
最小交叉反应
Cow, Mouse more details -
免疫原
Rabbit IgG
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偶联物
PE. Ex: 488nm, Em: 575nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.71% Sucrose, 0.27% sodium dihydrogen phosphate, 1.09% disodium hydrogen phosphate -
Concentration information loading...
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纯度
Size exclusion -
纯化说明
Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used. -
共轭说明
Excitation max. l 565>498 nm. Emission max. l 578 nm. F/P 0.8:1. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72465于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt | (1) |
1/500 - 1/1000.
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ELISA |
Use at an assay dependent concentration.
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说明 |
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Flow Cyt
1/500 - 1/1000. |
ELISA
Use at an assay dependent concentration. |
图片
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Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Goat anti-rabbit IgG H&L (Phycoerythrin, pre-adsorbed) (ab72465) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 575/30 bandpass filter.
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Sandwich ELISA to detect Rabbit IgG. Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated Rabbit IgG and read after a 2 hour incubation at room temperature with ab72465
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab72465 被引用在 17 文献中.
- Ou S et al. Tibial cortex transverse transport potentiates diabetic wound healing via activation of SDF-1/CXCR4 signaling. PeerJ 11:e15894 (2023). PubMed: 37727693
- Gamal-Eldeen AM et al. Anti-hypoxic Effect of Polysaccharide Extract of Brown Seaweed Sargassum dentifolium in Tongue Squamous Cell Carcinoma. Front Nutr 9:854780 (2022). PubMed: 35399691
- Gouhar SA et al. Involvement of miRNAs in response to oxidative stress induced by the steroidal glycoalkaloid α-solanine in hepatocellular carcinoma cells. Environ Toxicol 37:212-223 (2022). PubMed: 34655286
- Derkus B et al. Xenogenic Neural Stem Cell-Derived Extracellular Nanovesicles Modulate Human Mesenchymal Stem Cell Fate and Reconstruct Metabolomic Structure. Adv Biol (Weinh) 6:e2101317 (2022). PubMed: 35347890
- Qiang P et al. Esaxerenone inhibits the macrophage-to-myofibroblast transition through mineralocorticoid receptor/TGF-β1 pathway in mice induced with aldosterone. Front Immunol 13:948658 (2022). PubMed: 36148244