山羊抗兔IgG H&L (IRDye® 800CW)预吸附二抗(ab216773)
概述
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产品名称山羊抗兔IgG H&L (IRDye® 800CW)预吸附二抗
参阅全部 IgG 二抗 -
描述山羊抗兔IgG H&L (IRDye® 800CW)预吸附二抗
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宿主Goat
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靶标种属Rabbit
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特异性This antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA.
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经测试应用适用于: WB, In-Cell ELISAmore details
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最小交叉反应
Chicken, Human, Mouse, Rat, Sheep more details -
免疫原
Full length protein corresponding to Rabbit IgG.
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偶联物IRDye® 800CW
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
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存储溶液pH: 7.4
Preservative: 0.01% Sodium azide
Constituent: PBS
Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer. -
Concentration information loading... -
纯度Affinity purified
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纯化说明Affinity chromatography purified using pooled rabbit IgG covalently linked to agarose.
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克隆多克隆
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同种型IgG
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研究领域
应用
Our Abpromise guarantee covers the use of ab216773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/5000 - 1/25000. | |
| In-Cell ELISA | 1/200 - 1/1200. |
图片
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Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)All lanes : Anti-NF-kB p65 antibody (ab16502) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate (20 µg)
Lane 2 : NF-kB p65 knockout HAP1 cell lysate (20 µg)
Lane 3 : HeLa cell lysate (20 µg)
Lane 4 : A431 cell lysate (20 µg)
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution (Goat anti-Rabbit IgG H&L (IRDye® 800CW))This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab16502 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Merged signal (red and green). Green - ab16502 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776.
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Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)All lanes : Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1 µg/ml (Anti-Lamin B1 antibody - Nuclear Envelope Marker)
Lane 1 : Wild-type HAP1 nuclear lysate, 10 ug
Lane 2 : Lamin B1 knockout HAP1 nuclear lysate
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilutionThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab16048 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Merged signal (red and green). Green signal from target - ab16048 observed at 68 kDa. Red signal from loading control - ab10799 ( (loading control to histone H3 at 0.1µg/ml) observed at 18 kDa using as secondary Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776.
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Other - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)Excitation and emission spectrum of IRDye® 800CW Ex: 776nm, Em: 792nm.
文献
This product has been referenced in:
- Milner DS et al. Environment-dependent fitness gains can be driven by horizontal gene transfer of transporter-encoding genes. Proc Natl Acad Sci U S A 116:5613-5622 (2019). Read more (PubMed: 30842288) »
- Schwenzer H et al. Oxidative Stress Triggers Selective tRNA Retrograde Transport in Human Cells during the Integrated Stress Response. Cell Rep 26:3416-3428.e5 (2019). Read more (PubMed: 30893612) »
