Key features and details
- Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
- Conjugation: IRDye® 680RD
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, In-Cell ELISA
Related conjugates and formulations
产品名称山羊抗兔IgG H&L (IRDye® 680RD)预吸附二抗
参阅全部 IgG 二抗
描述山羊抗兔IgG H&L (IRDye® 680RD)预吸附二抗
经测试应用适用于: WB, In-Cell ELISAmore details
Chicken, Human, Mouse, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Full length protein corresponding to Rabbit IgG.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
存储溶液Preservative: 0.01% Sodium azide
Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer.
Concentration information loading...
纯化说明Affinity chromatography purified using pooled IgG covalently linked to agarose.
The Abpromise guarantee
1/5000 - 1/25000.
1/200 - 1/800.
1/5000 - 1/25000.
1/200 - 1/800.
All lanes : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) at 1/10000 dilution (Anti-GAPDH antibody)
Lane 1 : Wild-type HAP1 cell lysate (20 µg)
Lane 2 : Beta actin knockout HAP1 cell lysate (20 µg)
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution (Goat anti-Mouse IgG H&L (IRDye® 680RD))
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with Anti-GAPDH antibody ab181602 overnight at 4°C. Antibody binding was detected using the Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Image taken from In-cell ELISA PhosphoPDH Kit (ab110218), which uses Goat anti-mouse IgG H&L (IRDye® 680RD) preadsorbed ab216777 for detection among its components.
Excitation and emission spectra of IRDye® 680RD Ex: 680, Em:694.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab216777 被引用在 8 文献中.
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- Polaski JT et al. The origins and consequences of UPF1 variants in pancreatic adenosquamous carcinoma. Elife 10:N/A (2021). PubMed: 33404013
- Chien HC et al. PPARd and FOXO1 Mediate Palmitate-Induced Inhibition of Muscle Pyruvate Dehydrogenase Complex and CHO Oxidation, Events Reversed by Electrical Pulse Stimulation. Int J Mol Sci 21:N/A (2020). PubMed: 32824862
- Li Z et al. TRIM21-mediated proteasomal degradation of SAMHD1 regulates its antiviral activity. EMBO Rep 21:e47528 (2020). PubMed: 31797533
- Peacock TP et al. Swine ANP32A Supports Avian Influenza Virus Polymerase. J Virol 94:N/A (2020). PubMed: 32269123
- Sun K et al. Postretrieval Microinjection of Baclofen Into the Agranular Insular Cortex Inhibits Morphine-Induced CPP by Disrupting Reconsolidation. Front Pharmacol 11:743 (2020). PubMed: 32508658
- Elbaz-Alon Y et al. PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria. Nat Commun 11:3645 (2020). PubMed: 32686675