Application
Immunohistochemistry (Frozen sections)
Sections were thawed and fixed in cold acetone. Endogenous peroxidase and serum blocking (10% normal horse serum) were performed. Primary antibody was incubated with ab9722, at 5µg/ml overnight at 4°C. An HRP-conjugated secondary (ab97080 at 1/300 dilution) was used for 30min at room temperature. Sections were counterstained with haematoxylin and mounted with DPX.
A negative control was included using an isotype IgG at the same concentration as the primary antibody.
*Staining performed by Maria Wallert.
A negative control was included using an isotype IgG at the same concentration as the primary antibody.
*Staining performed by Maria Wallert.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Ana Maluenda
Verified customer
提交于 Jan 31 2018