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Immunology Immunoglobulins Heavy Chain IgG

山羊抗兔IgG H&L (HRP)预吸附二抗(ab97080)

  • Datasheet
  • SDS
Reviews (2)Q&A (21)References (115)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080)
  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080)

Key features and details

  • Goat Anti-Rabbit IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

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概述

  • 产品名称

    山羊抗兔IgG H&L (HRP)预吸附二抗
    参阅全部 IgG 二抗
  • 宿主

    Goat
  • 靶标种属

    Rabbit
  • 特异性

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.

     

  • 经测试应用

    适用于: ICC, IHC-P, ELISA, WBmore details
  • 最小交叉反应


    Chicken, Cow, Horse, Human, Mouse, Pig, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • 偶联物

    HRP

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 6.8
    Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Enzyme
    • HRP

相关产品

  • Substrate reagent

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    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab97080于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
Use at an assay dependent concentration.
IHC-P
1/200 - 1/5000.
ELISA
1/25000 - 1/75000.

as primary.

WB (1)
1/5000 - 1/50000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000.
说明
ICC
Use at an assay dependent concentration.
IHC-P
1/200 - 1/5000.
ELISA
1/25000 - 1/75000.

as primary.

WB
1/5000 - 1/50000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000.

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080)

    IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97080, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080)
    Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080)

    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution developed using the ECL technique, with ab87342.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (115)

发表研究结果有使用 ab97080?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab97080 被引用在 115 文献中.

  • Bazzar W  et al. Pharmacological inactivation of CDK2 inhibits MYC/BCL-XL-driven leukemia in vivo through induction of cellular senescence. Cell Cycle 20:23-38 (2021). PubMed: 33356836
  • Susantad T  et al. Modified recombinant human erythropoietin with potentially reduced immunogenicity. Sci Rep 11:1491 (2021). PubMed: 33452310
  • Li H  et al. Hsa_circ_0000199 facilitates chemo-tolerance of triple-negative breast cancer by interfering with miR-206/613-led PI3K/Akt/mTOR signaling. Aging (Albany NY) 13:4522-4551 (2021). PubMed: 33495420
  • Iacono D  et al. Reduction of pTau and APP levels in mammalian brain after low-dose radiation. Sci Rep 11:2215 (2021). PubMed: 33500491
  • Pralle RS  et al. Greater liver PNPLA3 protein abundance in vivo and in vitro supports lower triglyceride accumulation in dairy cows. Sci Rep 11:2839 (2021). PubMed: 33531537
View all Publications for this product

客户评价及客户问答

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提交评价 提交问题

1-10 of 23 Abreviews or Q&A

Nice secondary Ab with no non-specific binding (clean background)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
This item was applied as a secondary Ab for my WB. 1:10,000 dilution was applied for chemiluminescence reaction afterwards. I also conducted the procedure with secondary Ab probing only (right side of the blot) and the result showed no non-specific binding for this Ab. I am satisfied for this item in my WB assay.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 11 2018

IHC for Interleukin 1B in mouse aortic sinus, frozen sections

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sections were thawed and fixed in cold acetone. Endogenous peroxidase and serum blocking (10% normal horse serum) were performed. Primary antibody was incubated with ab9722, at 5µg/ml overnight at 4°C. An HRP-conjugated secondary (ab97080 at 1/300 dilution) was used for 30min at room temperature. Sections were counterstained with haematoxylin and mounted with DPX.
A negative control was included using an isotype IgG at the same concentration as the primary antibody.
*Staining performed by Maria Wallert.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Ana Maluenda

Verified customer

提交于 Jan 31 2018

Question

que signifie "pre-absorbed" svp ?

Read More

Abcam community

Verified customer

Asked on Jan 31 2013

Answer

Pré-adsorption est une étape supplémentaire pour augmenter la spécificité d'un anticorps. Je vous envoie un lien pour notre site internet qui explique ce terme en plus de détailes.

https://www.abcam.com/index.html?pageconfig=resource&rid=12956

Read More

Abcam Scientific Support

回复于 Jan 31 2013

Question

Which secondary antibody is suitable agianst rabbit anti chicked ab?

Read More

Abcam community

Verified customer

Asked on Jan 17 2013

Answer

Thank you for contacting us.

Please have a look at the troubleshooting guide given on our website;

https://www.abcam.com/index.html?pageconfig=resource&rid=11384

I am sorry I am unable to get the specific protocol for chicken tissues. My colleague from laboratory has suggested that any general IHC-P protocol would work and the staining can be improved with optimization.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

回复于 Jan 17 2013

Question

I am sending you a picture of my PVDF membrane. I only saw the bands at 36 and 56 KDa as Abacam's website mentioned.   The first lane (from left to right): Molecular weight marker                                                 Marker (from top to bottom): 250, 150, 100, 75, 50, 37, 25, 20, 15, 10 KDa The second lane: HELA (positive control) The remaining lanes: my samples (should contain p21)   What concentration of the secondary antibody should I try? How long should I let the signals develop? In this membrane, I let the signals developed for 1 hour as recommended by Abcam. However, the protein bands look very faint. So, should I let the signals develop for longer? I will try with concentration of 1:100 for the primary antibody and see how it turns out. I really hope that I can see p21 in HELA.

Read More

Abcam community

Verified customer

Asked on Dec 11 2012

Answer

Thank you for your reply.

While I did not receive the image which you have referenced I would like to offer so help regarding your questions. Generally specific details regarding secondary antibody incubation and concentration as will need to be determined empirically in the lab. These may vary considerable depending upon the secondary antibody used. This is the same for exposure times. We do offer a number of guidelines for information on our datasheet however. Please remember that these are suggested starting points and you will likely need to adjust these to find the optimal ranges.

The secondary antibody AB97080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) was used at 1/5000 dilution. In this experiment ECL detection was used with a 20 minute exposure time. This product may be found at this location:

https://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-hl-hrp-pre-adsorbed-ab97080.html

The secondary antibody AB65484 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) was used at 1/5000 dilution. In this experiment ECL detection was used with a 150 second exposure time. Unfortunately this product is no longer available.

A third experiment is shown on the datasheet; however we do not have specific information as to the product number of the secondary antibody used.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

回复于 Dec 11 2012

Question

Inquiry: Hi, I just received my secondary antibody. First off, it would be nice if you labeled your packages more precisely. It was labeled as 'Temperature sensitive - store according to datasheet instructions'. The problem with this is that the datasheet is inside the package and the people that sign for our packages do not open them, they just store them until they are picked up. So a +4 product should be labeled with +4 degrees. How sensitive is this antibody? It has been lying in room temperature for three days. Will this compromise the stability of the product or should it be fine? Also, is it OK to store this product at +4 degrees only or should it be aliquoted into smaller volumes and frozen at -20 degrees? Thank you for your help. Kind regards,

Read More

Abcam community

Verified customer

Asked on Sep 21 2012

Answer

Thank you for contacting us.

Abcam takes product quality seriously and we recently undertook an in-depth study examining storage conditions and antibody performance. After over 13,000 individual ELISA experiments, we have determined that storing our antibodies at various temperatures of up to 45°C for 1 week does not impact their activity. I would be happy to share a summary of our findings with you; please let me know if you would like to see this.

As a precautionary measure only, we ship our antibodies in packaging with ice packs to provide extra temperature stability in transit. Once you have received any products, we recommend toplease follow the long-term storage instructions on the datasheet as soon as possible. This will help to ensure that the product remains stable and that we can continue to provide our guarantee.

In this case, considering our findings described above, I would suggest that the antibody should not be significantly affected from being at room temperature for 3 days. We recently redesigned our packing. A decision was taken to label these with 'Temperature sensitive - store according to datasheet instructions' in order to cover all the different products we ship at different temperatures.This instruction should prompt the recipient to check the datasheet, or check with the end user, as soon as possible.

However, I do understand your concerns, and we encourage feedback from customers and take this seriously. I have therefore forwarded your comments on to my colleagues for their reference.

I would like to reassure you that our Abpromise to you is that, should our product not work as stated on the datasheet, we will resolve the issue to your satisfaction, either with helpful advice to optimize your experiment or a replacement or refund of the product. Please contact our knowledgeable Scientific Support staff should you experience any issues while using our products. Contact information can be found at:

https://www.abcam.com/index.html?pageconfig=contactus

I hope this information helps, but please don't hesitate to contact me for additional information or assistance.

Read More

Abcam Scientific Support

回复于 Sep 21 2012

Question

Look this image what we wanted to get. What I did:
1. I had increased pab incubation time 24hr at 4 0C.
2. I used sab 6721 1:200 dil(2mg/ml. 1:1000 dil was recommended.
Thanks again for kind help during IHC.

Read More

Abcam community

Verified customer

Asked on Sep 13 2012

Answer

Thank you for your email.
I'm glad to see that the antibody worked.
Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

回复于 Sep 13 2012

Question

I have received both secondary antibodies. Thank you very much.
-ab6721(2mg/ml)- In data sheet it is mentioned (Concentration 2mg/ml)for IHC 1:1000 recommended. So please let me know I should go 1:200 or 1:1000.
-ab97051(1mg/ml)- In data sheet it is mentioned (Concentration 1mg/ml)for IHC 1:200 recommended.
Today I am trying with both antibodies.
I am waiting reply as early as possible because I have to start the experiment.

Read More

Abcam community

Verified customer

Asked on Aug 28 2012

Answer

Thank you for your email.
I'd suggest starting with the recommended dilutions for each antibody.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

回复于 Aug 28 2012

Question

I am waiting for antibody. Could I know when I will receive.

Read More

Abcam community

Verified customer

Asked on Aug 27 2012

Answer

Thank you for your email.
The free of charge order was shipped today for delivery tomorrow.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

回复于 Aug 27 2012

Question

Thank you very much for taking so much care. Next week I will do again with new antibody which you are sending. Let us hope for the best. Definitely let you know the results.

Read More

Abcam community

Verified customer

Asked on Aug 24 2012

Answer

You are very welcome. I keep my fingers crossed for you and wish you good luck.
Yes, definitely, please keep me informed. I look forward to hear back from you - hopefully with positive results.
Have a nice weekend.

Read More

Abcam Scientific Support

回复于 Aug 24 2012

1-10 of 23 Abreviews or Q&A

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