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Immunology Immunoglobulins Heavy Chain IgG

山羊抗兔IgG H&L (FITC)预吸附二抗(ab7086)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (14)

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Key features and details

  • Goat Anti-Rabbit IgG H&L (FITC) preadsorbed
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA

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概述

  • 产品名称

    山羊抗兔IgG H&L (FITC)预吸附二抗
    参阅全部 IgG 二抗
  • 宿主

    Goat
  • 靶标种属

    Rabbit
  • 经测试应用

    适用于: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details
  • 最小交叉反应


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • 免疫原

    Full length native Rabbit IgG (purified).

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明

    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Fluorophore
    • FITC

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7086于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Immunomicroscopy
Flow Cyt
IHC-P
IHC-Fr (1)
ELISA
说明
应用说明
Flow Cyt: Use at an assay dependant concentration.
IHC-P: Use at an assay dependant concentration.
IHC-Fr: Use at an assay dependant concentration.
IM: Use at an assay dependant concentration.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (14)

发表研究结果有使用 ab7086?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7086 被引用在 14 文献中.

  • Xu Z  et al. Effects of shRNA-mediated silencing of PDE5A3 on intracellular cGMP and free Ca2+ levels and human prostate smooth muscle cell proliferation from benign prostatic hyperplasia. Exp Ther Med 21:322 (2021). PubMed: 33732295
  • Vardiani M  et al. Gelatin Electrospun Mat as a Potential Co-culture System for In Vitro Production of Sperm Cells from Embryonic Stem Cells. ACS Biomater Sci Eng 6:5823-5832 (2020). PubMed: 33320586
  • Amjadi F  et al. Comparative evaluation of NOTCH signaling molecules in the endometrium of women with various gynecological diseases during the window of implantation. Iran J Basic Med Sci 22:426-431 (2019). PubMed: 31168348
  • Wang X  et al. Elevated microRNA-145-5p increases matrix metalloproteinase-9 by activating the nuclear factor-?B pathway in rheumatoid arthritis. Mol Med Rep 20:2703-2711 (2019). PubMed: 31322192
  • Zhuang L  et al. Co-culturing with hypoxia pre-conditioned mesenchymal stem cells as a new strategy for the prevention of irradiation-induced fibroblast-to-myofibroblast transition. Oncol Rep 42:1781-1792 (2019). PubMed: 31485596
View all Publications for this product

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Good secondary antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Used as part of an ISH/IHC double stain on fresh frozen human skeletal muscle. Used at a 1/200 dilution to bind to the Abcam Caspase-3 primary antibody (ab52293).

Incubated at room temperature for 60 minutes in a dark staining chamber.

Abcam user community

Verified customer

提交于 Aug 01 2016

Question

My customer is looking for an anti-mouse secondary antibody with fluorophore conjugate which can be detected with blue filters (wavelength 460-490 nm) in his fluorescence microscope. He is also considering ab7086 (anti-rabbit FITC) for a second primary antibody for his double IF simultaneous protocol.

Please advise if ab47052 can be detected with this blue filter? If not, can you kindly recommend one?

Thank you.

Best regards,

Read More

Abcam community

Verified customer

Asked on Jul 19 2012

Answer

Thank you for your inquiry.

I can confirm that the ab47052 can be detected with this filter at 460 to 490nm. Indeed, the fluorochrome AMCA has a maximum emission at 445nm, however the fluorescent emission between 460 and 490nm should still be detectable. Obviously the signal would however be stronger if the customer would have a filter letting pass 445nm as well. Please note however that the ab47052 is an anti mouse antibody.

The ab7086 coupled to FITC can also be used as emission maximum of FITC is at 519nm. Even though the spectrum is quite large, the emissionis very low under 500nm. (http://en.wikipedia.org/wiki/Fluorescein)I am therefore positive that those two fluorochromes can be used together without any significant interference.

Here is also a link to the our guide to fluorescent dyes: https://docs.abcam.com/pdf/immunology/fluorochrome_guide.pdf

The only thing I would like to draw your attention to, is that the ab47052 has not been crossabsorbed. This means we do not know whether this antibody would also recognize the rabbit antibodies. For a double staining it is therefore imperative that the customer makes this control (using only primary rabbit antibody and secondary anti mouse antibody to see whether there is any crossreaction). We unfortunately do not have another anti mouse antibody with a "blue" fluorochrome which would be already pre-absorbed. The ab7086 howeverhas been preabsorbed and is therefore perfectly fine for the double staining.

I hope this information is helpful. Please do not hesitate to get back to us, should you have any additional questions.

Read More

Abcam Scientific Support

回复于 Jul 19 2012

Question

Our lab is about to start doing immunohistochemistry on frozen sections of mouse brain with primary antibody from rabbit. He have access to a confocal microscope and a fluorescent microscope with a UV lamp. Which antibody conjugation would allow for the easiest and best signal detection (ab7086, ab6720, etc.)? Also, I have rviewed your protocols for IHC, and find all the various adjustments confusing. If you can make a recommendation, can you also please indicate which protocol should be followed? Thank you.

Read More

Abcam community

Verified customer

Asked on Jun 24 2005

Answer

Thank you for your enquiry. First, when selecting a secondary antibody conjugated to a fluorochrome, you're limited by the available microscope filter sets that go with your microscope. Most filter sets are best matched with rhodamine or fluorescein. Texas Red® may also be used with a rhodamine filter set. So you want to determine which filter sets that you have and then choose an appropriate secondary to go along with your primary antibody. On our website we have a nice chart with various fluorochromes and their corresponding wavelengths. Just select the resources tab and then click on antibody guide from the drop-down menu. Then choose "choosing an antibody" and click go. I would recommend following the 2 Step Fluorescent protocol that we have listed on the IHC - Frozen sections / Cytological preparations protocols section on our website. A really good IHC website (IHC World) that you may want to refer to is below. http://www.ihcworld.com/introduction.htm It has a lot of good protocols and details, and should be helpful for you. If you have any additional questions, please do contact us again.

Read More

Abcam Scientific Support

回复于 Jun 28 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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