Key features and details
- Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, WB, ELISA, Dot blot, ICC/IF, IHC-Fr, ICC
存放说明Shipped at 4°C. Store at +4°C.
存储溶液Preservative: 0.1% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
共轭说明Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Our Abpromise guarantee covers the use of ab6722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
|WB||1/1000 - 1/4000.|
|ELISA||1/3000 - 1/5000.|
|Dot blot||Use at an assay dependent dilution.|
|ICC/IF||1/200 - 1/1000.|
|IHC-Fr||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent dilution.|
Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody.
Lane M: 3 µl Molecular ladder.
Lane 1: Rabbit IgG whole molecule.
Lane 2: Rabbit IgG F(ab) Fragment.
Lane 3: Rabbit IgG F(c) Fragment.
Lane 4: Rabbit IgM Whole Molecule.
Lane 5: Normal Rabbit Serum.
All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.
Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 for 30 min at RT.
Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.
Rabbit F(c) migrates slightly higher.
Dot Blot of ab6722.
Antigen: Rabbit IgG.
Load: Lane 1 - 200ng; Lane 2 - 66.7ng; Lane 3 - 22.2ng; Lane 4 - 7.4ng; Lane 5 - 2.5ng.
Secondary antibody: ab6722 used at 1:1,000 for 60 min at RT. Blocked for 60 min at RT.
Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.
Lane 1: Rabbit IgG.
Load: 100 ng per lane.
Secondary antibody: ab6722 used at 1:1,000 for 60 min at RT. Blocked for 30 min at RT.
Predicted/Observed size: 55 and 28 kDa, 55 kDa for Rabbit IgG.
ab6722 被引用在 27 文献中.
- De Clercq K et al. Double-label immunohistochemistry to assess labyrinth structure of the mouse placenta with stereology. Placenta 94:44-47 (2020). PubMed: 32421534
- Jayamohan S et al. Dysregulation of miR-375/AEG-1 Axis by Human Papillomavirus 16/18-E6/E7 Promotes Cellular Proliferation, Migration, and Invasion in Cervical Cancer. Front Oncol 9:847 (2019). PubMed: 31552174
- Kannan M et al. AEG-1/miR-221 Axis Cooperatively Regulates the Progression of Hepatocellular Carcinoma by Targeting PTEN/PI3K/AKT Signaling Pathway. Int J Mol Sci 20:N/A (2019). PubMed: 31698701
- Subramanian K et al. Pneumolysin binds to the mannose receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival. Nat Microbiol 4:62-70 (2019). PubMed: 30420782
- Wang Y et al. Electroacupuncture treatment upregulates a7nAChR and inhibits JAK2/STAT3 in dorsal root ganglion of rat with spared nerve injury. J Pain Res 12:1947-1955 (2019). PubMed: 31308727