山羊抗兔IgG H&L (Alkaline Phosphatase) (ab6722)
Key features and details
- Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, WB, ELISA, Dot blot, ICC/IF, IHC-Fr, ICC
Related conjugates and formulations
概述
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产品名称
山羊抗兔IgG H&L (Alkaline Phosphatase)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
经测试应用
适用于: IHC-P, WB, ELISA, Dot blot, ICC/IF, IHC-Fr, ICCmore details -
免疫原
Rabbit IgG whole molecule
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偶联物
Alkaline Phosphatase
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent dilution.
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WB |
1/1000 - 1/4000.
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ELISA |
1/3000 - 1/5000.
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Dot blot |
Use at an assay dependent dilution.
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|
ICC/IF |
1/200 - 1/1000.
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IHC-Fr |
Use at an assay dependent dilution.
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ICC |
Use at an assay dependent dilution.
|
说明 |
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IHC-P
Use at an assay dependent dilution. |
WB
1/1000 - 1/4000. |
ELISA
1/3000 - 1/5000. |
Dot blot
Use at an assay dependent dilution. |
ICC/IF
1/200 - 1/1000. |
IHC-Fr
Use at an assay dependent dilution. |
ICC
Use at an assay dependent dilution. |
图片
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Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody.
Lane M: 3 µl Molecular ladder.
Lane 1: Rabbit IgG whole molecule.
Lane 2: Rabbit IgG F(ab) Fragment.
Lane 3: Rabbit IgG F(c) Fragment.
Lane 4: Rabbit IgM Whole Molecule.
Lane 5: Normal Rabbit Serum.
All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.
Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 for 30 min at RT.
Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.
Rabbit F(c) migrates slightly higher.
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Dot Blot of ab6722.
Antigen: Rabbit IgG.
Load: Lane 1 - 200ng; Lane 2 - 66.7ng; Lane 3 - 22.2ng; Lane 4 - 7.4ng; Lane 5 - 2.5ng.
Secondary antibody: ab6722 used at 1:1,000 for 60 min at RT. Blocked for 60 min at RT.
Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.
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Lane 1: Rabbit IgG.
Load: 100 ng per lane.
Secondary antibody: ab6722 used at 1:1,000 for 60 min at RT. Blocked for 30 min at RT.
Predicted/Observed size: 55 and 28 kDa, 55 kDa for Rabbit IgG.
数据表及文件
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SDS download
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Datasheet download
文献 (40)
ab6722 被引用在 40 文献中.
- Wawrzykowski J et al. The comparison of pro- and antioxidative parameters in plasma and placental tissues during early phase of placental development in cows. Mol Biol Rep 48:1291-1297 (2021). PubMed: 33507474
- Hao J et al. Curcumin suppresses colorectal tumorigenesis via the Wnt/ß-catenin signaling pathway by downregulating Axin2. Oncol Lett 21:186 (2021). PubMed: 33574925
- Palomino J et al. Embryo Buoyancy and Cell Death Gene Expression During Embryogenesis of Yellow-Tail Kingfish Seriola lalandi. Front Cell Dev Biol 9:630947 (2021). PubMed: 33816479
- Lee JS et al. A Comparison of Isolation Stress and Unpredictable Chronic Mild Stress for the Establishment of Mouse Models of Depressive Disorder. Front Behav Neurosci 14:616389 (2020). PubMed: 33488368
- Gritsyna YV et al. Inhibition of Histone Deacetylases 4 and 5 Reduces Titin Proteolysis and Prevents Reduction of TTN Gene Expression in Atrophied Rat Soleus Muscle after Seven-Day Hindlimb Unloading. Dokl Biochem Biophys 495:338-341 (2020). PubMed: 33368047