Application
Immunocytochemistry/ Immunofluorescence
I performed this on fixed cells in 4 % (w/v) formaldehyde. For the detection of CAR protein, I used CAR (Ab83850) at 1:50 dilution, and secondary antibody Goat Anti-Rabbit lgG H&L (Alexa Fluor 188) preadsorbed (ab150081). These are the optimum antiody dilutions for my result. The distribution of green fluorescent signals are CAR protein.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Yu Siong Ho
Verified customer
提交于 Aug 03 2020