为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Immunology Immunoglobulins Heavy Chain IgG

山羊抗兔IgG H&L (Alexa Fluor® 488)预吸附二抗(ab150081)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (44)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
  • Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
  • Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
  • Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)

Key features and details

  • Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA

你可能会感兴趣

细胞
Product image
ioGlutamatergic Neurons – Human iPSC-Derived Glutamatergic Neurons (ab259259)
封闭
Product image
Normal Goat Serum (ab7481)

查看更多相关产品

概述

  • 产品名称

    山羊抗兔IgG H&L (Alexa Fluor® 488)预吸附二抗
    参阅全部 IgG 二抗
  • 描述

    山羊多克隆抗体二抗to兔IgG - H&L (Alexa Fluor® 488),预吸附二抗
  • 宿主

    Goat
  • 靶标种属

    Rabbit
  • 特异性

    By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, horse, human, mouse, pig, and rat IgG was detected. This antibody may cross react with IgG from other species.

     

  • 经测试应用

    适用于: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details
  • 最小交叉反应


    Human, Mouse, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 偶联物

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Antiserum was cross adsorbed using a human, mouse and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 常规说明

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Fluorophore
    • Alexa Fluor® 488

相关产品

  • Matched antibody pairs

    • Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
  • Related Products

    • Anti-CD3 antibody [SP7] (ab16669)
    • ioGlutamatergic Neurons - Human iPSC-Derived Glutamatergic Neurons (ab259259)
    • Normal Goat Serum (ab7481)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab150081于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr (1)
Use at an assay dependent concentration.
ICC/IF (3)
1/200 - 1/1000.
Flow Cyt
1/2000 - 1/4000.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
说明
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
Flow Cyt
1/2000 - 1/4000.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

图片

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)

    ICC/IF image of ab8227 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8227, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab150081 Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)

    ab92742 staining Ki67 in wild-type HAP1 cells (top panel) and Ki67 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab92742 at 1µg/ml and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 µg/ml (shown in green). Nuclear DNA was labeled in blue with DAPI.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)

    Unpurified ab134175 staining Cyclin D1 in MCF7 (Human breast adenocarcinoma cell line) cells treated with KN-93 (ab120980).
    The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab134175 at 10µg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Goat anti-Rabbit Alexa 488 secondary (ab150081) at 2 µg/ml (shown in green) and Goat anti-Mouse Alexa 594 secondary (ab150120) at 2 µg/ml (shown in pseudo color red). Nuclear DNA was labeled in blue with DAPI.
    Negative controls: 1- Rabbit primary and anti-mouse secondary antibody; 2 - Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)This image is courtesy of an abreview submitted by Bryan Niedenberger

    Postnatal day 6 mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in 0.1% Triton X-100 + PBS. Sections were incubated with either (A) no primary antibody or (B ) anti-DDX4 (ab13840) for 1 h at RT. Sections were then washed 3X with 0.1% Triton X-100 in PBS and Goat-Anti Rabbit 488 (ab150081) applied at a 1/500 dilution. Sections were then mounted after washing 3X with 0.1% Triton X-100 in PBS.

  • Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)This image is courtesy of Dr. Shaohua Li

    Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

    Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    Fix in 3%PFA in PBS for 30 min at RTIncubate in 7.5% sucrose-PBS for 3h at RTIncubate in 15% sucrose-PBS at 4 degree Celsius overnightEmbed the EBs in tissue-Tek OCT compoundCut frozen sections to 4-20 µm thickness

    Primary antibody 1: Rabbit anti cytokeratin 8 (ab53280), 1:100

    Primary antibody 2: Rat anti-perlecan, 1:100 
    Secondary antibody 1: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) pre-adsorbed (ab150081), 1:200

    Secondary antibody 2: Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5®) pre-adsorbed, 1:200
    Nuclei were counterstained with DAPI 

  • Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (44)

发表研究结果有使用 ab150081?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab150081 被引用在 44 文献中.

  • Kakuta T  et al. Parathyroid hormone-producing cells exist in adipose tissues surrounding the parathyroid glands in hemodialysis patients with secondary hyperparathyroidism. Sci Rep 10:3290 (2020). PubMed: 32094398
  • Gu L  et al. Preparation and characterization of methacrylated gelatin/bacterial cellulose composite hydrogels for cartilage tissue engineering. Regen Biomater 7:195-202 (2020). PubMed: 32296538
  • Lei W  et al. The IAP Antagonist SM-164 Eliminates Triple-Negative Breast Cancer Metastasis to Bone and Lung in Mice. Sci Rep 10:7004 (2020). PubMed: 32332865
  • Chen R  et al. Promoter-Level Transcriptome Identifies Stemness Associated With Relatively High Proliferation in Pancreatic Cancer Cells. Front Oncol 10:316 (2020). PubMed: 32266133
  • Kang LJ  et al. 3'-Sialyllactose prebiotics prevents skin inflammation via regulatory T cell differentiation in atopic dermatitis mouse models. Sci Rep 10:5603 (2020). PubMed: 32221370
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-5 of 5 Abreviews or Q&A

investigate the distribution of CAR protein in porcine brain endothelial cells.

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
I performed this on fixed cells in 4 % (w/v) formaldehyde. For the detection of CAR protein, I used CAR (Ab83850) at 1:50 dilution, and secondary antibody Goat Anti-Rabbit lgG H&L (Alexa Fluor 188) preadsorbed (ab150081). These are the optimum antiody dilutions for my result. The distribution of green fluorescent signals are CAR protein.

Dr. Yu Siong Ho

Verified customer

提交于 Aug 03 2020

good working antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
We used the antibody together with the ab38578 antibody (Siglec8) on cytospins with eosinophilic granulocytes.
The working concentration was 1/100 for 30 minutes at room temperature.

Abcam user community

Verified customer

提交于 Feb 14 2018

Excellent results

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
I have used this antibody at a 1:500 dilution with many different primary antibodies. It works well and and gives a bright signal with no discernible nonspecific staining.
Postnatal day 6 mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in 0.1% Triton X-100 + PBS. Sections were incubated with either (A) no primary antibody or (B ) anti-DDX4 (Abcam ab13840) for 1 h at RT. Sections were then washed 3X with 0.1% Triton X-100 in PBS and Goat-Anti Rabbit 488 (Abcam ab150081) applied at a 1:500 dilution. Sections were then mounted after washing 3X with 0.1% Triton X-100 in PBS.

Mr. Bryan Niedenberger

Verified customer

提交于 Mar 09 2016

Indirect Immunofluorescent labeling of a nuclear lamin protein

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
This antibody has been used extensively with a number of different primary antibodies generated in rabbits. It performs extremely well at a 1:200 dilution.
Attached is an immunofluorescent image of MeOH-fixed HeLa cells that were immunofluorescently labeled with a Lamin A/C (nuclear membrane) antibody (ab133256). The secondary antibody has been pseudo-colored green in the merged image. DNA was counterstained with DAPI.

Dr. Kirk Mcmanus

Verified customer

提交于 Mar 28 2014

Good secondary a.b. for IHC (PFA-Fixed Flash-Frozen sections)

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry
Application: Immunohistochemistry (PFA perfusion fixed frozen sections) or IHC(FoFr)

I used this antibody on PFA fixed, sucrose cryoprotected frozen sections from the mouse brain (below is a brief description). The primary a.b. I used was anti-mGluR5 (ab53090), which was somewhat difficult to get working.

Full Description:
Transcardially perfused the mouse with 4% PFA. Dissected the brain and placed in PFA for a few hours later, placed in 30% sucrose overnight for cryoprotection. Then, snap/flash froze the brain and used a cryostat for sectioning into 20 micron coronal slices. Applied primary anti-mGluR5 (ab53090) antibody, followed by the secondary antibody (dilution: 1/200), according to standard protocols. No blocking, no antigen-retrieval, and no permeabilization was done.

Abcam user community

Verified customer

提交于 Jan 29 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2021 艾博抗(上海)贸易有限公司版权所有