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Immunology Immunoglobulins Heavy Chain IgG

山羊抗兔IgG H&L (Alexa Fluor® 488) (ab150077)

  • Datasheet
  • SDS
Reviews (16)Q&A (1)References (702)

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • IHC - Wholemount - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

Key features and details

  • Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488)
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, ELISA, IHC-Fr

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Product image
Normal Goat Serum (ab7481)

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概述

  • 产品名称

    山羊抗兔IgG H&L (Alexa Fluor® 488)
    参阅全部 IgG 二抗
  • 描述

    山羊多克隆抗体二抗to兔IgG - H&L (Alexa Fluor® 488)
  • 宿主

    Goat
  • 靶标种属

    Rabbit
  • 特异性

    This antibody is specific to Rabbit IgG.

  • 经测试应用

    适用于: ICC/IF, Flow Cyt, IHC-P, ELISA, IHC-Frmore details
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 偶联物

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 常规说明

    Fluorochrome chart – a complete guide:

    A quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

    Please here.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Fluorophore
    • Alexa Fluor® 488

相关产品

  • Alternative Versions

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175471)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175773)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)
    • Goat Anti-Rabbit IgG H&L (ab182016)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)
  • Related Products

    • Normal Goat Serum (ab7481)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF (6)
1/200 - 1/1000.
Flow Cyt
1/2000 - 1/4000.
IHC-P (2)
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-Fr (4)
Use at an assay dependent concentration.
说明
ICC/IF
1/200 - 1/1000.
Flow Cyt
1/2000 - 1/4000.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

图片

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

    ICC/IF image of beta Tubulin staining in HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab6046, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab150077 Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Immunocytochemistry/ Immunofluorescence analysis of C6(Rat glial tumor glial cell) cells labeling alpha smooth muscle Actin with purified ab32575 at 1/100 dilution (0.71 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Overlay histogram showing MCF7 cells stained with unpurified ab32063 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32063, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3(Mouse embryonic fibroblast) cells labeling alpha smooth muscle Actin with purified ab32575 at 1/500 dilution (5.2 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling Estrogen Receptor alpha with purified ab32063 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab7291, a mouse anti-tubulin (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary antibody. Nuclei counterstained with DAPI (blue).
    Control 1: primary antibody (1/1000) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Immunocytochemistry/Immunofluorescence analysis of Raji (human Burkitt's lymphoma) cells labelling HLA A with purified ab52922 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150077) was used at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ATG9A with Purified ab108338 at 1/100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma) cells labeling alpha smooth muscle Actin (green) with purified ab32575 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counterstained with ab7291, anti-Tubulin (mouse mAb) at 1/1000 followed by ab150120 Alexa Fluor®594 goat anti-mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).
    For negative control 1, rabbit primary antibody and anti-mouse secondary antibody (ab150120) were used. For negative control 2, ab7291 (mouse primary antibody) was used followed by anti-rabbit secondary antibody (ab150077).
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling IL-1RA with purified ab124962 at 1/100. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

    The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) and (ab16048, 1µg/ml) overnight at +4°C. The secondary antibodies were ab150115 Alexa Fluor® 647 (red) goat anti-mouse IgG (H+L) used at 2µg/ml for 1h and ab150077 Alexa Fluor® 488 (green) goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei.

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

    Overlay histogram showing HeLa cells stained with ab32356 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32356, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

    Cross-reactivity of the polyclonal secondary antibody ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182016 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.

    For the batch tested, ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.

    This data was developed using the unconjugated antibody (ab182016).

  • ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

    Cross-reactivity of Goat anti-Rabbit IgG H&L (ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

    This data was developed using the unconjugated antibody (ab182016).

  • IHC - Wholemount - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    IHC - Wholemount - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)This image is courtesy of an anonymous Abreview.

    IHC - Wholemount of Caenorhabditis elegans larvae labelling RNA polymerase II CTD repeat YSPTSPS (phospho S2) with ab5095. The sample was incubated with primary antibody (1/500 in PBS + 3% BSA + 0.1% Triton X-100) for 12 hours at 4°C. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000), was used as the secondary antibody.

    See Abreview

  • Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (702)

发表研究结果有使用 ab150077?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab150077 被引用在 702 文献中.

  • Hozain AE  et al. Multiday maintenance of extracorporeal lungs using cross-circulation with conscious swine. J Thorac Cardiovasc Surg 159:1640-1653.e18 (2020). PubMed: 31761338
  • Quan M  et al. Relationships Between Disc Degeneration and Autophagy Expression in Human Nucleus Pulposus. Orthop Surg 12:312-320 (2020). PubMed: 31802633
  • Zhang Y  et al. Knockout of beta-2 microglobulin reduces stem cell-induced immune rejection and enhances ischaemic hindlimb repair via exosome/miR-24/Bim pathway. J Cell Mol Med 24:695-710 (2020). PubMed: 31729180
  • Kim DK  et al. Elastase-Positive Neutrophils Are Associated With Refractoriness of Chronic Rhinosinusitis With Nasal Polyps in an Asian Population. Allergy Asthma Immunol Res 12:42-55 (2020). PubMed: 31743963
  • Mao P  et al. PDZ-Binding Kinase-Dependent Transcriptional Regulation of CCNB2 Promotes Tumorigenesis and Radio-Resistance in Glioblastoma. Transl Oncol 13:287-294 (2020). PubMed: 31874375
View all Publications for this product

客户评价及客户问答

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提交评价 提交问题

1-10 of 17 Abreviews or Q&A

Immunostaining of transferrin with anti-rabbit antibody (ab150077)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample was a liver slide (FFPE-tissue)
Primary antibody: Rabbit anti-Transferrin
Secondary antibody: Goat anti-rabbit IgG H&L (Alexa Fluor® 488)Abcam ab150077
Blocking buffer: 5% Goat serum
Incubation of secondary antibody: 1:500 at RT for 30 min
Strong fluorescent signal with moderate background noise was observed.

Abcam user community

Verified customer

提交于 Feb 26 2021

Immunofluorescence

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
HeLa cells were imaged using ab150077 as a secondary antibody.

Fix cells for 5 min with 4% paraformaldehyde, permeabilize for 5 min with 0.1% Triton-X 100

Primary antibody: ab39688, MYC, 1:100 dilution, RT, 90 min

Secondary antibody: ab150077, 1:500 dilution, RT, 45 min

Abcam user community

Verified customer

提交于 Feb 05 2021

Secondary antibody anti rabbit-AlexaFluor488 works excellent!

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Used for IF staining of kryo frozen prostate cancer slides.
Primary antibody: TIMP1 (ab211926).
Gives great signal at 1:500 dilution, minimal background.
Inc. time primary and secondary: 30 min at 21°C

Mrs. Simone Grad

Verified customer

提交于 Dec 14 2020

Worked well with Anti-GRP78 BiP antibody ab21685

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
This Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) worked well with Anti-GRP78 BiP antibody ab21685 in deer mice skin sections.

Abcam user community

Verified customer

提交于 Aug 21 2020

Immunocytochemical staining (HeLa cells)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Cell: HeLa
Fix & permeabilization: 4% formalin, 0.03% Triton-X100
Blocking: 3% BSA/PBS
1st antibody: anti-Nup133 Rabbit polyclonal antibody (1/500 dil, 3% BSA/PBS)
2nd antibody: Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) (1/2500 dil, 3% BSA/PBS)

Abcam user community

Verified customer

提交于 Feb 24 2020

Detection of CD3 in FFPE section with secondary anti-rabbit-AF488

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
The secondary antibody works well. Based on my experience for IHC-P the dilution factor requires optimization based on the tissue and antigen retrieval method used.

The dilution 1:250 works in my case.

Abcam user community

Verified customer

提交于 Feb 17 2020

Very good antibody for indirect staining!

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Immunocytochemistry/ Immunofluorescence
Resulted in bright and specific staining.
Cells: PBMCs
Fixation: Acetone
Primary antibody: CD3
Blocking: 5% BSA in PBS
Counterstained with DAPI

Abcam user community

Verified customer

提交于 Feb 12 2020

Great secondary antibody, bright and specific staining

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Melanoma tissue was stained to see CD3 infiltrates.
Tissue was fixed with acetone for 10 min at -20 °C.
The intensity of the fluorescence was really great.
No Background signal was detected.
I strongly can recommend this antibody.

Abcam user community

Verified customer

提交于 Feb 12 2020

a-tubulin (488) and actin (555) staining on primary rabbit intestinal epithelial cells

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Application: Immunocytochemistry
Cells were fixed in 2% Paraformaldehyde.
Permeabilization: Triton X-100
Primary antibody: Rabbit polyclonal anti-a-tubulin
Blocking: 5% BSA in PBS
Counterstained with DAPI and actin (555)

Dr. Egi Kardia

Verified customer

提交于 Dec 05 2019

An abreview for ab150077 - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
HeLa cells were cultured on cover slips and fixed with 4% paraformaldehyde in PBS (pH7.4) at room temperature for 10 min after exposed upon 10 uM choloroquine for 6 h. After washing three times with PBS, the cells were permeabilized with ice-cold methanol for 2.5 min. After three washes with PBS, the cells were incubated with blocking solution (5% BSA in PBS) for 1 h and then with primary antibody LC3 overnight at 4ºC. The next day, the cells were washed five times for 5 min each time with PBS and then incubated with secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) for 1 to 2 h. The cells were washed four times with PBS for 5 min each time, and DAPI stained for 15 min. After three washes with PBS, the coverslips were mounted on slides using Fluoro-GEL (Electron Microscopy Sciences). Images were taken using Keyence BZ-X700.

Abcam user community

Verified customer

提交于 Nov 01 2018

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