山羊抗兔IgG H&L (Agarose) (ab7019)

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Key features and details

  • Goat Anti-Rabbit IgG H&L (Agarose)
  • Conjugation: Agarose
  • Host species: Goat
  • Suitable for: IHC-P, IHC-Fr, IP

概述

  • 产品名称

    山羊抗兔IgG H&L (Agarose)
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Rabbit

  • 经测试应用

    适用于: IHC-P, IHC-Fr, IPmore details

  • 免疫原

    Rabbit IgG whole molecule

  • 偶联物

    Agarose

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.1% Sodium azide
    Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate
  • Concentration information loading...

  • 纯度

    IgG fraction

  • 纯化说明

    This product is an IgG fraction antibody coupled to activated agarose.

  • 共轭说明

    Coupled to cyanogen bromide activated cross-linked 4% agarose. 10.0 mg antibody per cc agarose

  • 克隆

    多克隆

  • 常规说明


    Agarose conjugated antibodies are designed for the rapid and efficient collection of antibodies and proteins, including recombinant fusion proteins, from a complex protein mixture (see protocol). Antibody affinity gels are useful for selection of immunoglobulins and immunoprecipitation of antigens from sera, antisera, ascitic fluid or culture fluid, bacterial and mammalian cell lysates and fusion protein preparations.

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7019于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

Sufficient antibody capacity is provided to bind a minimum of 5 mg of pure rabbit IgG.
AP
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

Sufficient antibody capacity is provided to bind a minimum of 5 mg of pure rabbit IgG.
AP
Use at an assay dependent concentration.

文献 (0)

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客户评价及客户问答

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Question

Customer is using this as a negative control and has found that when incubating with the secondary only, she is seeing a black blot with white bands. However, the first couple of times, this did not happen.

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Answer

Thank you for your enquiry and patience. Usually when a reverse image is seen, it is due to too much HRP in the system and it is suggested to dilute the HRP-conjugate at least 10-fold. It could also be due to the antibody settling over time, so that when you go to use it later on, it is more concentrated at the bottom. Please make sure that the product is gently mixed prior to using. I hope this helps, if you have any additional questions please contact us again.

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