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Immunology Immunoglobulins Heavy Chain IgM

山羊抗小鼠IgM mu chain预吸附二抗(ab98671)

  • Datasheet
Submit a review Q&A (2)

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Abpromise

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Key features and details

  • Goat Anti-Mouse IgM mu chain preadsorbed
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, ELISA, WB, ICC

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 647 Alkaline Phosphatase APC Biotin DyLight® 488 DyLight® 594 FITC Gold 18nm Gold 6nm HRP Unconjugated

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概述

  • 产品名称

    山羊抗小鼠IgM mu chain预吸附二抗
    参阅全部 IgM 二抗
  • 宿主

    Goat
  • 靶标种属

    Mouse
  • 经测试应用

    适用于: IHC-P, ELISA, WB, ICCmore details
  • 最小交叉反应


    Human, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgM
    • Secondary antibodies
    • anti-Mouse
    • IgM
    • Unconjugated
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab98671于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/200 - 1/2000.
ELISA
1/100 - 1/30000. Coating: 1/100 - 1/500. Primary: 1/1000 - 1/30000.
WB
1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000.
ICC
Use at an assay dependent dilution.
说明
IHC-P
1/200 - 1/2000.
ELISA
1/100 - 1/30000. Coating: 1/100 - 1/500. Primary: 1/1000 - 1/30000.
WB
1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000.
ICC
Use at an assay dependent dilution.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (0)

发表研究结果有使用 ab98671?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab98671 尚未被引用在任何文献中。

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Dear technical team, Our customer has ab21035(IgM), ab97230(IgG) and is going to conjugate FITC.   She wants to know that antibody has -COOH or -NH2. I know generally whole antibodies have -cooH group at the Fc region but don't know about -NH2.   Check this for our customer.    

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Abcam community

Verified customer

Asked on Dec 13 2011

Answer

Thank you for your inquiry. Indeed, antibodies as most (unmodified) proteins have a carboxy group (-COOH) at their C-terminus, as you indicated. They also have an amine group (-NH2) at their N-terminus. Furthermore, antibodies contain amino acids which have carboxy and amine groups in their side-chains, such as Arginine, Lysine, Glutamate or Aspartate. Therefore, the coupling reaction with FITC that usually takes place via free amine groups forming a stable thiourea bond (as for example with our EasyLink kits) is absolutely feasible for the anti-Enrofloxacin/Ciprofloxacin antibody ab21035. However, as the Goat polyclonal Secondary antibody ab97230 is already conjugated to HRP, I would not recommend to label this antibody with FITC. As alternative, I would suggest using an unconjugated antibody such as ab98671: Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgM-mu-chain-preadsorbed-ab98671.html). We also have a Goat polyclonal Secondary antibody to Mouse IgM (mu chain) available that is already FITC-conjugated (ab97229): Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgM-mu-chain-FITC-ab97229.html). I hope this information is helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

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Abcam Scientific Support

回复于 Dec 13 2011

Question

Dear Thank you for your reply.I would like to order mouse secondary IgM antibody that is specific only to IgM and not other immunoglobulins.  I would like to first start with western blotting as I have measured the bands for IgG and IgE and I will do ELISA later after detecting any change in the western blot.  Thank you for your assistance! Best regards,

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Abcam community

Verified customer

Asked on Sep 02 2011

Answer

Thank you for your reply. Both antibodies suggested in my last email (ab98671 and ab98743) that were suitable for ELISA should also detect IgM in denatured or reduced forms when being used as primary antibody in WB. We also offer ab98679 which is HRP labelled and could be used directly without need for a secondary antibody that then is HRP labelled. In this case it is not necessary to use an F(ab’)2 antibody. I hope this information was helpful. Please do not hesitate to contact me again with any further questions.

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Abcam Scientific Support

回复于 Sep 02 2011

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