山羊抗小鼠IgG3 heavy chain (Biotin) (ab97258)
Key features and details
- Goat Anti-Mouse IgG3 heavy chain (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, ELISA, WB
概述
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产品名称
山羊抗小鼠IgG3 heavy chain (Biotin)
参阅全部 IgG3 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG3. Cross reactivity with other immunoglobulins is less than 2%. -
经测试应用
适用于: IHC-P, ICC/IF, ELISA, WBmore details -
偶联物
Biotin
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97258于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/250 - 1/2500.
|
|
ICC/IF |
1/100 - 1/500.
|
|
ELISA |
1/10000 - 1/200000. (Primary).
|
|
WB |
1/10000 - 1/200000. (Chemiluminescent).
|
说明 |
---|
IHC-P
1/250 - 1/2500. |
ICC/IF
1/100 - 1/500. |
ELISA
1/10000 - 1/200000. (Primary). |
WB
1/10000 - 1/200000. (Chemiluminescent). |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab97258 被引用在 2 文献中.
- Fu Q et al. CCL21 activation of the MALAT1/SRSF1/mTOR axis underpins the development of gastric carcinoma. J Transl Med 19:210 (2021). PubMed: 34001131
- Prost S et al. Choice of Illumination System & Fluorophore for Multiplex Immunofluorescence on FFPE Tissue Sections. PLoS One 11:e0162419 (2016). ICC/IF . PubMed: 27632367