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山羊抗小鼠IgG2b heavy chain (FITC)预吸附二抗(ab98702)

Submit a review Q&A (3)References (2)

Key features and details

  • Goat Anti-Mouse IgG2b heavy chain (FITC) preadsorbed
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: Flow Cyt, ICC/IF, IHC-P

Conjugates logo Related conjugates and formulations

APC/Cy7® Biotin HRP PE/Cy7® Unconjugated Unconjugated

概述

  • 产品名称

    山羊抗小鼠IgG2b heavy chain (FITC)预吸附二抗
    参阅全部 IgG2b 二抗

  • 宿主

    Goat

  • 靶标种属

    Mouse

  • 经测试应用

    适用于: Flow Cyt, ICC/IF, IHC-Pmore details

  • 最小交叉反应


    Human, Rat more details

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab98702于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
1/50 - 1/200.
ICC/IF
1/50 - 1/500.
IHC-P
1/50 - 1/500.
说明
Flow Cyt
1/50 - 1/200.
ICC/IF
1/50 - 1/500.
IHC-P
1/50 - 1/500.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (2)

发表研究结果有使用 ab98702？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab98702 被引用在 2 文献中.

  • Takahashi N  et al. Hypoxia Inducible Factor 1 Alpha Is Expressed in Germ Cells throughout the Murine Life Cycle. PLoS One 11:e0154309 (2016). PubMed: 27148974
  • Yigit S  et al. Bioengineered silk gene delivery system for nuclear targeting. Macromol Biosci 14:1291-8 (2014). PubMed: 24889658

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

Inquiry: Hi, I would like to buy your: ab41349 dengue antibody. Would it be possible to know which secondary antibody is likely to go with it please? It would be good if it is conjugated to FITC. This is to be used for IF. Best wishes

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Answer

Thank you for contacting us.

We searched on our web page and there are three products that I could recommend to you. They are all three against the IgG2b of mouse and were used for ICC/IF. The species the three products were raised in is different and that might be important for you and will need some consideration. For full information about our products please see the three data sheets below:


https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG2b-heavy-chain-FITC-ab97249.html


https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG2b-heavy-chain-FITC-pre-adsorbed-ab98702.html

https://www.abcam.com/Rat-monoclonal-LO-MG2b-2-Secondary-Antibody-to-Mouse-IgG2b-heavy-chain-FITC-ab99637.html


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Inquiry: Good morning- I am inquiring about an intracellular staining protocol for the anti-gp100 antibody (ab117853) with the FITC-conjugated anti-mouse IgG2b (ab98702). There was a reference made to some staining protocols but I could not locate the actual protocols. Please see below for a portion of a post that I found on the website. 'For gp100, we have unconjugated antibodies but neither is in a formulation that is compatible with the conjugation kits for FITC. What I recommend is using the unconjugated anti-gp100, ab117853, followed by FITC-conjugated anti-mouse IgG2b, ab98702, pre-adsorbed to remove reactivity with mouse IgG1 (which is the isotype of ab52460, anti-cytokeratin).' Thank you for your help!

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Answer

Thank you for your inquiry and your interest in our antibodies. The recommendation you found on the website, was specific in regards to make a double staining with another specific antibody.

I can recommend you to follow the classical intracellular staining protocol for using ab117853 as per the link here. The antibody should work perfectly well in these conditions. Optimal dilution should be determined by you in the laboratory, as a starting point I would recommend dilutions of 1/50, 1/100 and 1/200 as we had good results with 1/100 dilution.

The secondary antibody can be used in a first test at 1/50 to be sure to be saturating. When you have determined the optimal dilution of the primary antibody, I can recommend to test whether the secondary antibody can also be further diluted.

Here is the link to the intracellular flow cytometry protocol:
https://www.abcam.com/index.html?pageconfig=resource&rid=11448

I hope this information is helpful. Please do not hesitate to contact us again, should you have any further question.

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Question


Request you to please let me know whether abcam
can supply the following antibodies (reactivity:
human)
1. Fluorescein isothiocyanate (FITC)-conjugated
antibody against the melanosome-specific protein
gp100
2. Phycoerythrin (PE)-conjugated antibody against
the keratinocyte-specific marker cytokeratin.
3. PE conjugated antibody against collagen 1, type
1
All are used for flowcytometry. Antibodies 1&2
will be used together

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Answer

Thank you for contacting us.

We have a PE- conjugated antibody against cytokeratin, catalogue item ab52460:

Click here (or use the following: https://www.abcam.com/PE-pan-Cytokeratin-antibody-C-11-ab52460.html).

For collagen I, we have only one unconjugated antibody that has been tested in flow cytometry. We do, however, have a kit that enables one to easily conjugate the antibody to PE.

Collagen I - ab23446
EasyLink PE - ab102919 or ab102918

For gp100, we have unconjugated antibodies but neither is in a formulation that is compatible with the conjugation kits for FITC. What I recommend is using the unconjugated anti-gp100, ab117853, followed by FITC-conjugated anti-mouse IgG2b, ab98702, pre-adsorbed to remove reactivity with mouse IgG1 (which is the isotype of ab52460, anti-cytokeratin). This should allow you to apply both antibodies to the same sample without cross-reaction of the secondary anti-IgG2b with mouse IgG1.

Click here (or use the following: https://www.abcam.com/Melanoma-gp100-antibody-OTI7E3-ab117853.html).

Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG2b-heavy-chain-FITC-preadsorbed-ab98702.html).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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