山羊抗小鼠IgG1 (HRP) (ab97240)
Key features and details
- Goat Anti-Mouse IgG1 (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, ELISA, IHC-P, WB
概述
-
产品名称
山羊抗小鼠IgG1 (HRP)
参阅全部 IgG1 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. This antibody may cross react with IgG1 from other species. -
经测试应用
适用于: ICC, ELISA, IHC-P, WBmore details -
偶联物
HRP
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
共轭说明
Molar enzyme/ antibody protein ratio is 4:1 -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97240于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC |
1/200 - 1/2000.
|
|
ELISA |
1/10000 - 1/100000.
|
|
IHC-P |
1/200 - 1/2000.
|
|
WB | (1) |
1/2000 - 1/20000. Colorimetric method; 1/2000 - 1/20000 Chemiluminescence method.
|
说明 |
---|
ICC
1/200 - 1/2000. |
ELISA
1/10000 - 1/100000. |
IHC-P
1/200 - 1/2000. |
WB
1/2000 - 1/20000. Colorimetric method; 1/2000 - 1/20000 Chemiluminescence method. |
图片
-
IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11080, 7.5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97240, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
All lanes : Anti-Topoisomerase I antibody (ab58313)
Lane 1 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 1 minute.
Lane 2 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 2 minutes.
Lane 3 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 4 minutes.
Lane 4 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 8 minutes.
Lane 5 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 1 minute.
Lane 6 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 2 minutes.
Lane 7 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 4 minutes.
Lane 8 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 8 minutes.
Secondary
All lanes : Goat Anti-Mouse IgG1 (HRP) (ab97240)The band corresponding to the enzyme–substrate cleaved complex, has been indicated by an asterisk.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (126)
ab97240 被引用在 126 文献中.
- Maniar R et al. Self-Renewing CD8+ T-cell Abundance in Blood Associates with Response to Immunotherapy. Cancer Immunol Res 11:164-170 (2023). PubMed: 36512052
- Chen S et al. Assembly of Immunogenic Protein Particles toward Advanced Synthetic Vaccines. Small 19:e2205819 (2023). PubMed: 36564365
- Weyant KB et al. A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens. Nat Commun 14:464 (2023). PubMed: 36709333
- Stoler-Barak L et al. B cell class switch recombination is regulated by DYRK1A through MSH6 phosphorylation. Nat Commun 14:1462 (2023). PubMed: 36927854
- Walker MWG et al. Novel molecular requirements for CRISPR RNA-guided transposition. Nucleic Acids Res 51:4519-4535 (2023). PubMed: 37078593