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山羊抗小鼠IgG1 heavy chain (Alkaline Phosphatase) (ab97237)

Submit a review Q&A (2)References (4)

Key features and details

  • Goat Anti-Mouse IgG1 heavy chain (Alkaline Phosphatase)
  • Conjugation: Alkaline Phosphatase
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

Conjugates logo Related conjugates and formulations

APC APC/Cy7® Biotin FITC Unconjugated Unconjugated

概述

  • 产品名称

    山羊抗小鼠IgG1 heavy chain (Alkaline Phosphatase)
    参阅全部 IgG1 二抗

  • 宿主

    Goat

  • 靶标种属

    Mouse

  • 特异性

    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%.

  • 经测试应用

    适用于: ICC, IHC-P, ELISA, WBmore details

  • 偶联物

    Alkaline Phosphatase

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).

  • 共轭说明

    Molar enzyme: antibody protein ratio is 1:1

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab97237于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
Use at an assay dependent dilution.
IHC-P
1/100 - 1/1000.
ELISA
1/1000 - 1/10000.
WB
1/1000 - 1/10000. Use chemiluminescence.
说明
ICC
Use at an assay dependent dilution.
IHC-P
1/100 - 1/1000.
ELISA
1/1000 - 1/10000.
WB
1/1000 - 1/10000. Use chemiluminescence.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (4)

发表研究结果有使用 ab97237？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab97237 被引用在 4 文献中.

  • Thomas S  et al. Development of a new fusion-enhanced oncolytic immunotherapy platform based on herpes simplex virus type 1. J Immunother Cancer 7:214 (2019). PubMed: 31399043
  • Lynch JM  et al. Binding of a glaucoma-associated myocilin variant to the aB-crystallin chaperone impedes protein clearance in trabecular meshwork cells. J Biol Chem 293:20137-20156 (2018). PubMed: 30389787
  • Lynch JM  et al. Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle. PLoS One 13:e0206801 (2018). PubMed: 30395621
  • Aguilar-Rodríguez J  et al. The Molecular Chaperone DnaK Is a Source of Mutational Robustness. Genome Biol Evol 8:2979-2991 (2016). PubMed: 27497316

客户评价及客户问答

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Question

I have an antibody which works , but I am looking for an HRP tagged one :- Goat Anti-Mouse IgG1 heavy chain (Alkaline Phosphatase) (ab97237).

I was wondering if you had the exact same antibody with HRP tag.

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Answer

We do have the same antibody conjugated with HRP: Goat Anti-Mouse IgG1 heavy chain (HRP) (https://www.abcam.com/ab97240)

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Question

Thank you very much for your helpful information and time. I am looking for anti-mouse IgG2a and IgG1 secondary antibodies (either HRP- or AP- conjugated) to use for Isotyping of specific mouse serum IgGs against my desired antigen. I searched through your products but I could not find any product. I should say that the secondary antibodies I need must not cross-react with other mouse IgG subtypes. By the way, if you have any mouse IgG isotyping ELISA kit, I really appreciate it if you let me know. I am looking forward to hearing from you. Best Wishes,

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Answer

Thank you very much for your email. I have compiled here below a list of antibodies which I think could correspond to your needs. There are three different IgG1 and three different IgG2a antibodies which have been tested against other isotypes. Please have a look at them and do not hesitate to contact me again should you need any more information. The Rat monoclonal [SB77e] Secondary Antibody to Mouse IgG1 (HRP), pre-adsorbed (ab99603) has been tested by ELISA against a panel of purified Mouse Immunoglobulins including IgM, IgG1, IgG2a, IgG2b, IgG3, and IgA. This product is positive for IgG1 and has minimal reactivity with the other Mouse Immunoglobulins. https://www.abcam.com/Rat-monoclonal-SB77e-Mouse-IgG1-HL-HRP-preadsorbed-ab99603.html Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (AP) (ab97237) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Alkaline-Phosphatase-ab97237.html Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (HRP) (ab97240) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. This antibody may cross react with IgG1 from other species https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Alkaline-Phosphatase-ab97237.html Rat monoclonal [LO-MG2a-9] Secondary Antibody to Mouse IgG2a - heavy chain (HRP) (ab11571) ab11571 recognises murine gamma 2a immunoglobulin heavy chain, and does not cross-react with other murine immunoglobulin classes or subclasses. https://www.abcam.com/Rat-monoclonal-Mouse-IgG2a-heavy-chain-HRP-LO-MG2a-9-ab11571.html Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (HRP) (ab97245) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/Goat-Mouse-IgG2a-heavy-chain-HRP-ab97245.html Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (AP) (ab97242) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/Goat-Mouse-IgG2a-heavy-chain-Alkaline-Phosphatase-ab97242.html Unfortunately we do not have an immunoglobulin isotyping ELISA kit for the moment in our catalog. I have passed this feedback on and I hope we will have one in the future. I hope this information is helpful. Please do not hesitate to contact me again should you have any other question. I wish you a great weekend!

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