山羊抗小鼠IgG H&L (TRITC) (ab6786)

Submit a review Q&A (2)References (28)

Key features and details

  • Goat Anti-Mouse IgG H&L (TRITC)
  • Conjugation: TRITC. Ex: 547nm, Em: 572nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA

概述

  • 产品名称

    山羊抗小鼠IgG H&L (TRITC)
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Mouse

  • 经测试应用

    适用于: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details

  • 免疫原

    Mouse IgG whole molecule

  • 偶联物

    TRITC. Ex: 547nm, Em: 572nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 共轭说明

    Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt 1/2000 - 1/4000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000. (PubMed:15659422)
ELISA 1/10000 - 1/50000.

图片

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (TRITC) (ab6786)
    Flow Cytometry - Goat Anti-Mouse IgG H&L (TRITC) (ab6786)
    Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (TRITC) (ab6786) was used at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 561nm laser and 610/20 bandpass filter.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (28)

发表研究结果有使用 ab6786？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6786 被引用在 28 文献中.

  • Yu F  et al. Programmed death ligand-1, tumor infiltrating lymphocytes and HLA expression in Chinese extrahepatic cholangiocarcinoma patients: Possible immunotherapy implications. Biosci Trends 13:58-69 (2019). PubMed: 30773525
  • Hong KH  et al. Fine-Tunable and Injectable 3D Hydrogel for On-Demand Stem Cell Niche. Adv Sci (Weinh) 6:1900597 (2019). PubMed: 31508277
  • Zheng C  et al. Inhibition of STAT3 in tubular epithelial cells prevents kidney fibrosis and nephropathy in STZ-induced diabetic mice. Cell Death Dis 10:848 (2019). PubMed: 31699972
  • Boric K  et al. Expression of apoptotic and proliferation factors in gastric mucosa of patients with systemic sclerosis correlates with form of the disease. Sci Rep 9:18461 (2019). PubMed: 31804582
  • Wang Z  et al. A Phage Lysin Fused to a Cell-Penetrating Peptide Kills Intracellular Methicillin-Resistant Staphylococcus aureus in Keratinocytes and Has Potential as a Treatment for Skin Infections in Mice. Appl Environ Microbiol 84:N/A (2018). PubMed: 29625989
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-2 of 2 Abreviews or Q&A

Question

I bought the primary antibody (Primary antibody (mouse anti CK3): https://www.abcam.com/Cytokeratin-3-antibody-AE5-ab77869.html) and secondary antibody (goat anti-mouse 1)https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-FITC-pre-adsorbed-ab97039.html
2) https://www.abcam.com/Mouse-IgG-secondary-antibody-H-L-ab6786.html) from you.

And I used rabbit cornea as the control and I tried several times for the positive control and negative control. But the results were weird.










All the slides which stained with FITC were positive (for negative control also showed positive staining)

All the slides which stained with TRITC were negative (for positive control also showed negative staining)



And I attach the immunostaining fotos and the protocol what I used for the immunostaing in the attachment. Could you please help me check if the antibodies what I chosen are not proper or the protocol what I used is not proper?? If it is, please send me a proper protocol for the immunostaining ! Thank you very much for your help!

Read More
Answer

Thank you for contacting Abcam.

I will be happy to help you further in this matter, but I just have a few more questions for you:

1 - Were you staining tissue sections or a monolayer of cultured cells?

2 - If you were staining sections, how were they prepared (PfA fixation & paraffin embedded, MeOH etc)?

3 - If these samples were paraffin embedded, what type of antigen retrieval did you use on them?

4 - Have you used either of the secondary antibodies successfully with other primary antibodies?

I look forward to your reply and helping to resolve these issues.

Read More

Question

I would just like to know if your secondary antibody ab6786 (goat polyclonal to mouse IgG H&L conjugated to Rhodamine) would be suitable in conjunction with antibody ab8329 (EBNA 1 antibody, mouse monoclonal IgG2b). It is not listed in your list of recommended secondaries for this antibody, but I cannot see from the data sheet why it would not work, Please advise,

Read More
Answer

Thank you for your enquiry. Yes, ab6786 - Goat polyclonal to Mouse IgG H&L (rhodamine) - would be a suitable secondary antibody to use with ab8329 - Mouse monoclonal [E1-2.5] to EBNA 1. The list of compatible secondaries located at the bottom of the datasheets are just suggestions, and don't encompass all of the secondary antibodies that we have that can be used with that particular primary antibody. If you have any additional questions, please let us know.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com