山羊抗小鼠IgG H&L (TRITC) (ab6786)

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Flow Cytometry - Goat Anti-Mouse IgG H&L (TRITC) (ab6786)

    Key features and details

    • Goat Anti-Mouse IgG H&L (TRITC)
    • Conjugation: TRITC. Ex: 547nm, Em: 572nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA

    概述

    • 产品名称

      山羊抗小鼠IgG H&L (TRITC)
      参阅全部 IgG 二抗

    • 宿主

      Goat

    • 靶标种属

      Mouse

    • 经测试应用

      适用于: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details

    • 免疫原

      Mouse IgG whole molecule

    • 偶联物

      TRITC. Ex: 547nm, Em: 572nm

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
    • Concentration information loading...

    • 纯度

      Affinity purified

    • 纯化说明

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • 共轭说明

      Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6786于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    1/2000 - 1/4000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    1/1000 - 1/5000. (PubMed:15659422)
    ELISA
    1/10000 - 1/50000.
    说明
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    1/2000 - 1/4000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    1/1000 - 1/5000. (PubMed:15659422)
    ELISA
    1/10000 - 1/50000.

    图片

    • Flow Cytometry - Goat Anti-Mouse IgG H&L (TRITC) (ab6786)
      Flow Cytometry - Goat Anti-Mouse IgG H&L (TRITC) (ab6786)
      Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (TRITC) (ab6786) was used at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 561nm laser and 610/20 bandpass filter.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (33)

    发表研究结果有使用 ab6786？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab6786 被引用在 33 文献中.

    • Molla S  et al. PARP inhibitor Olaparib Enhances the Apoptotic Potentiality of Curcumin by Increasing the DNA Damage in Oral Cancer Cells through Inhibition of BER Cascade. Pathol Oncol Res 26:2091-2103 (2020). PubMed: 31768967
    • Chen X  et al. The Nephroprotective Effect of TNF Receptor-Associated Factor 6 (TRAF6) Blockade on LPS-Induced Acute Renal Injury Through the Inhibition if Inflammation and Oxidative Stress. Med Sci Monit 26:e919698 (2020). PubMed: 31929494
    • Chen X  et al. AZD4547 Attenuates Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting Inflammation: The Role of FGFR1 in Renal Tubular Epithelial Cells. Drug Des Devel Ther 14:833-844 (2020). PubMed: 32161443
    • Chatterjee S & Kundu CN Nanoformulated quinacrine regulates NECTIN-4 domain specific functions in cervical cancer stem cells. Eur J Pharmacol 883:173308 (2020). PubMed: 32603697
    • Racetin A  et al. Expression and localization of DAB1 and Reelin during normal human kidney development. Croat Med J 60:521-531 (2019). PubMed: 31894918
    View all Publications for this product

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    I bought the primary antibody (Primary antibody (mouse anti CK3): https://www.abcam.com/Cytokeratin-3-antibody-AE5-ab77869.html) and secondary antibody (goat anti-mouse 1)https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-FITC-pre-adsorbed-ab97039.html
    2) https://www.abcam.com/Mouse-IgG-secondary-antibody-H-L-ab6786.html) from you.

    And I used rabbit cornea as the control and I tried several times for the positive control and negative control. But the results were weird.










    All the slides which stained with FITC were positive (for negative control also showed positive staining)

    All the slides which stained with TRITC were negative (for positive control also showed negative staining)



    And I attach the immunostaining fotos and the protocol what I used for the immunostaing in the attachment. Could you please help me check if the antibodies what I chosen are not proper or the protocol what I used is not proper?? If it is, please send me a proper protocol for the immunostaining ! Thank you very much for your help!

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    Answer

    Thank you for contacting Abcam.

    I will be happy to help you further in this matter, but I just have a few more questions for you:

    1 - Were you staining tissue sections or a monolayer of cultured cells?

    2 - If you were staining sections, how were they prepared (PfA fixation & paraffin embedded, MeOH etc)?

    3 - If these samples were paraffin embedded, what type of antigen retrieval did you use on them?

    4 - Have you used either of the secondary antibodies successfully with other primary antibodies?

    I look forward to your reply and helping to resolve these issues.

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    Question

    I would just like to know if your secondary antibody ab6786 (goat polyclonal to mouse IgG H&L conjugated to Rhodamine) would be suitable in conjunction with antibody ab8329 (EBNA 1 antibody, mouse monoclonal IgG2b). It is not listed in your list of recommended secondaries for this antibody, but I cannot see from the data sheet why it would not work, Please advise,

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    Answer

    Thank you for your enquiry. Yes, ab6786 - Goat polyclonal to Mouse IgG H&L (rhodamine) - would be a suitable secondary antibody to use with ab8329 - Mouse monoclonal [E1-2.5] to EBNA 1. The list of compatible secondaries located at the bottom of the datasheets are just suggestions, and don't encompass all of the secondary antibodies that we have that can be used with that particular primary antibody. If you have any additional questions, please let us know.

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