山羊抗小鼠IgG H&L (IRDye® 800CW)预吸附二抗(ab216772)

概述

  • 产品名称

    山羊抗小鼠IgG H&L (IRDye® 800CW)预吸附二抗
    参阅全部 IgG 二抗
  • 描述

    山羊抗小鼠IgG H&L (IRDye® 800CW)预吸附二抗
  • 宿主

    Goat
  • 靶标种属

    Mouse
  • 特异性

    This antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b and IgG3, and with the light chains of mouse IgM and IgA.
  • 经测试应用

    适用于: In-Cell ELISA, WBmore details
  • 最小交叉反应


    Goat, Horse, Human, Rabbit, Rat more details
  • 免疫原

    Full length protein corresponding to Mouse IgG.

  • 偶联物

    IRDye® 800CW

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • 存储溶液

    pH: 7.4
    Preservative: 0.01% Sodium azide
    Constituent: PBS

    Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    Affinity chromatography purified using pooled mouse IgG covalently linked to agarose.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab216772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
In-Cell ELISA 1/200 - 1/1200.
WB 1/5000 - 1/25000.

图片

  • All lanes : Anti-p53 antibody [DO-1] - ChIP Grade (ab1101) at 1/1000 dilution (Anti-p53 antibody [DO-1])

    Lane 1 : Wild-type HAP1 cell lysate (20 µg)
    Lane 2 : p53 knockout HAP1 cell lysate (20 µg)
    Lane 3 : A431 cell lysate (20 µg)
    Lane 4 : Saos-2 cell lysate (20 µg)

    Secondary
    All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/10000 dilution


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab1101 overnight at 4°C. Antibody binding was detected using the Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 at a 1/10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    Image shows merged signal (red and green). Green - ab1101 observed at 53 kDa. Red - GAPDH loading control, ab181602, observed at 37 kDa using as secondary Goat Anti-Rabbit IgG H&L (IRDye® 680RD)- ab216777

  • All lanes : Anti-beta Actin antibody [AC-15] (ab6276) at 1/5000 dilution (Anti-beta Actin antibody [AC-15])

    Lane 1 : Wild-type HAP1 cell lysate (20 µg)
    Lane 2 : Beta actin knockout HAP1 cell lysate (20 µg)

    Secondary
    All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/10000 dilution


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab6276 overnight at 4°C. Antibody binding was detected using the Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 at a 1/10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    Image shows merged signal (red and green). Green - ab6276 observed at 42 kDa. Red - GAPDH loading control, ab181602, observed at 37 kDa using as secondary Goat Anti-Rabbit IgG H&L (IRDye® 680RD)- ab216777

  • Image taken from In-cell ELISA PhosphoPDH Kit (ab110218), which uses Goat anti-mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 for detection among its components.
  • Excitation and emission spectrum of IRDye® 800CW Ex: 776nm, Em: 792nm.

文献

This product has been referenced in:

  • Ding M  et al. Schisandrin B protects human keratinocyte-derived HaCaT cells from tert-butyl hydroperoxide-induced oxidative damage through activating the Nrf2 signaling pathway. Int J Mol Med 42:3571-3581 (2018). Read more (PubMed: 30272282) »
  • Zhong L  et al. Arsenic trioxide induces the apoptosis and decreases NF-?B expression in lymphoma cell lines. Oncol Lett 16:6267-6274 (2018). Read more (PubMed: 30333888) »
See all 2 Publications for this product

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