Application
Western blot
Secondary antibody (ab 6789) was used at a dilution of 1:5000 in 5% BSA. Placental lysate was used. primary antibody was purchased for beta actin from pierce antibody.
gel: 10%, 200 V for 35 min.
transfer 17 V for 35 min. using Biorad TURBO
Blocking 5% BSA.
gel: 10%, 200 V for 35 min.
transfer 17 V for 35 min. using Biorad TURBO
Blocking 5% BSA.
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提交于 Nov 07 2016